Distribution of fluoride and calcium in plaque biofilms after the use of conventional and low-fluoride dentifrices

Juliano Pelim Pessan, Karina Mirela Ribeiro Pinto Alves, Flávia de Moraes Italiani, Irene Ramires, José Roberto Pereira Lauris, Gary M. Whitford, Kyriacos Jack Toumba, Colin Robinson, Marilia Afonso Rabelo Buzalaf

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: The distribution of fluoride and calcium in plaque after the use of fluoride dentifrices has not yet been determined. Aim: To evaluate fluoride and calcium distribution in sections of biofilms generated in situ after the use of conventional and low-fluoride dentifrices. Design: Children (n = 11, 8-10 years old) brushed with placebo (fluoride-free), low-fluoride (513 mgF/kg), and conventional (1072 mgF/kg) dentifrices twice daily for 1 week, following a double-blind, cross-over protocol. Biofilms were generated using Leeds in situ devices, which were collected 1 and 12 h after brushing, and sectioned through their depth. Sections were grouped (10 × 5 μm) for fluoride and calcium analysis. Sections 4 μm thick were used for image analysis and determination of biomass fraction. Results were analysed by anova, Tukey's test, and linear regression analysis (P < 0.05). Results: Fluoride and calcium were mostly located at the outer sections of biofilms for all dentifrices tested, and these ions were directly correlated throughout most of biofilm's sections. Results for conventional dentifrice were significantly higher than for the placebo, but did not differ from those for the low-fluoride dentifrice. Conclusions: The use of a low-fluoride dentifrice did not promote a higher fluoride uptake in inner biofilms' sections, as hypothesized. As plaque fluoride was significantly elevated only after the use of the conventional dentifrice, the recommendation of low-fluoride formulations should be done with caution, considering both risks and benefits.

Original languageEnglish (US)
Pages (from-to)293-302
Number of pages10
JournalInternational Journal of Paediatric Dentistry
Volume24
Issue number4
DOIs
StatePublished - Jan 1 2014

Fingerprint

Calcium Fluoride
Dentifrices
Biofilms
Fluorides
Placebos
Biomass
Linear Models
Regression Analysis
Ions

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Distribution of fluoride and calcium in plaque biofilms after the use of conventional and low-fluoride dentifrices. / Pessan, Juliano Pelim; Alves, Karina Mirela Ribeiro Pinto; Italiani, Flávia de Moraes; Ramires, Irene; Lauris, José Roberto Pereira; Whitford, Gary M.; Toumba, Kyriacos Jack; Robinson, Colin; Buzalaf, Marilia Afonso Rabelo.

In: International Journal of Paediatric Dentistry, Vol. 24, No. 4, 01.01.2014, p. 293-302.

Research output: Contribution to journalArticle

Pessan, JP, Alves, KMRP, Italiani, FDM, Ramires, I, Lauris, JRP, Whitford, GM, Toumba, KJ, Robinson, C & Buzalaf, MAR 2014, 'Distribution of fluoride and calcium in plaque biofilms after the use of conventional and low-fluoride dentifrices', International Journal of Paediatric Dentistry, vol. 24, no. 4, pp. 293-302. https://doi.org/10.1111/ipd.12073
Pessan, Juliano Pelim ; Alves, Karina Mirela Ribeiro Pinto ; Italiani, Flávia de Moraes ; Ramires, Irene ; Lauris, José Roberto Pereira ; Whitford, Gary M. ; Toumba, Kyriacos Jack ; Robinson, Colin ; Buzalaf, Marilia Afonso Rabelo. / Distribution of fluoride and calcium in plaque biofilms after the use of conventional and low-fluoride dentifrices. In: International Journal of Paediatric Dentistry. 2014 ; Vol. 24, No. 4. pp. 293-302.
@article{07bc437660cd4903b97e889e452bcb1a,
title = "Distribution of fluoride and calcium in plaque biofilms after the use of conventional and low-fluoride dentifrices",
abstract = "Background: The distribution of fluoride and calcium in plaque after the use of fluoride dentifrices has not yet been determined. Aim: To evaluate fluoride and calcium distribution in sections of biofilms generated in situ after the use of conventional and low-fluoride dentifrices. Design: Children (n = 11, 8-10 years old) brushed with placebo (fluoride-free), low-fluoride (513 mgF/kg), and conventional (1072 mgF/kg) dentifrices twice daily for 1 week, following a double-blind, cross-over protocol. Biofilms were generated using Leeds in situ devices, which were collected 1 and 12 h after brushing, and sectioned through their depth. Sections were grouped (10 × 5 μm) for fluoride and calcium analysis. Sections 4 μm thick were used for image analysis and determination of biomass fraction. Results were analysed by anova, Tukey's test, and linear regression analysis (P < 0.05). Results: Fluoride and calcium were mostly located at the outer sections of biofilms for all dentifrices tested, and these ions were directly correlated throughout most of biofilm's sections. Results for conventional dentifrice were significantly higher than for the placebo, but did not differ from those for the low-fluoride dentifrice. Conclusions: The use of a low-fluoride dentifrice did not promote a higher fluoride uptake in inner biofilms' sections, as hypothesized. As plaque fluoride was significantly elevated only after the use of the conventional dentifrice, the recommendation of low-fluoride formulations should be done with caution, considering both risks and benefits.",
author = "Pessan, {Juliano Pelim} and Alves, {Karina Mirela Ribeiro Pinto} and Italiani, {Fl{\'a}via de Moraes} and Irene Ramires and Lauris, {Jos{\'e} Roberto Pereira} and Whitford, {Gary M.} and Toumba, {Kyriacos Jack} and Colin Robinson and Buzalaf, {Marilia Afonso Rabelo}",
year = "2014",
month = "1",
day = "1",
doi = "10.1111/ipd.12073",
language = "English (US)",
volume = "24",
pages = "293--302",
journal = "International Journal of Paediatric Dentistry",
issn = "0960-7439",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Distribution of fluoride and calcium in plaque biofilms after the use of conventional and low-fluoride dentifrices

AU - Pessan, Juliano Pelim

AU - Alves, Karina Mirela Ribeiro Pinto

AU - Italiani, Flávia de Moraes

AU - Ramires, Irene

AU - Lauris, José Roberto Pereira

AU - Whitford, Gary M.

AU - Toumba, Kyriacos Jack

AU - Robinson, Colin

AU - Buzalaf, Marilia Afonso Rabelo

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Background: The distribution of fluoride and calcium in plaque after the use of fluoride dentifrices has not yet been determined. Aim: To evaluate fluoride and calcium distribution in sections of biofilms generated in situ after the use of conventional and low-fluoride dentifrices. Design: Children (n = 11, 8-10 years old) brushed with placebo (fluoride-free), low-fluoride (513 mgF/kg), and conventional (1072 mgF/kg) dentifrices twice daily for 1 week, following a double-blind, cross-over protocol. Biofilms were generated using Leeds in situ devices, which were collected 1 and 12 h after brushing, and sectioned through their depth. Sections were grouped (10 × 5 μm) for fluoride and calcium analysis. Sections 4 μm thick were used for image analysis and determination of biomass fraction. Results were analysed by anova, Tukey's test, and linear regression analysis (P < 0.05). Results: Fluoride and calcium were mostly located at the outer sections of biofilms for all dentifrices tested, and these ions were directly correlated throughout most of biofilm's sections. Results for conventional dentifrice were significantly higher than for the placebo, but did not differ from those for the low-fluoride dentifrice. Conclusions: The use of a low-fluoride dentifrice did not promote a higher fluoride uptake in inner biofilms' sections, as hypothesized. As plaque fluoride was significantly elevated only after the use of the conventional dentifrice, the recommendation of low-fluoride formulations should be done with caution, considering both risks and benefits.

AB - Background: The distribution of fluoride and calcium in plaque after the use of fluoride dentifrices has not yet been determined. Aim: To evaluate fluoride and calcium distribution in sections of biofilms generated in situ after the use of conventional and low-fluoride dentifrices. Design: Children (n = 11, 8-10 years old) brushed with placebo (fluoride-free), low-fluoride (513 mgF/kg), and conventional (1072 mgF/kg) dentifrices twice daily for 1 week, following a double-blind, cross-over protocol. Biofilms were generated using Leeds in situ devices, which were collected 1 and 12 h after brushing, and sectioned through their depth. Sections were grouped (10 × 5 μm) for fluoride and calcium analysis. Sections 4 μm thick were used for image analysis and determination of biomass fraction. Results were analysed by anova, Tukey's test, and linear regression analysis (P < 0.05). Results: Fluoride and calcium were mostly located at the outer sections of biofilms for all dentifrices tested, and these ions were directly correlated throughout most of biofilm's sections. Results for conventional dentifrice were significantly higher than for the placebo, but did not differ from those for the low-fluoride dentifrice. Conclusions: The use of a low-fluoride dentifrice did not promote a higher fluoride uptake in inner biofilms' sections, as hypothesized. As plaque fluoride was significantly elevated only after the use of the conventional dentifrice, the recommendation of low-fluoride formulations should be done with caution, considering both risks and benefits.

UR - http://www.scopus.com/inward/record.url?scp=84902115255&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84902115255&partnerID=8YFLogxK

U2 - 10.1111/ipd.12073

DO - 10.1111/ipd.12073

M3 - Article

C2 - 25045768

AN - SCOPUS:84902115255

VL - 24

SP - 293

EP - 302

JO - International Journal of Paediatric Dentistry

JF - International Journal of Paediatric Dentistry

SN - 0960-7439

IS - 4

ER -