Diurnal rhythmic expression of the rhythm-related genes, rPeriod1, rPeriod2, and rClock, in the rat brain

Kun Ruey Shieh, Shu Chuan Yang, Xinyun Lu, Huda Akil, Stanley J. Watson

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

High densities of the mRNA of three rhythm-related genes, rPeriod1 (rPer1), rPer2, and rClock, which share high homology in Drosophila and mammals, are found in the rat hypothalamic suprachiasmatic nucleus (SCN). The SCN, however, is not the only brain region that expresses these genes. To understand the possible physiological roles of these rhythm-related genes, we examined expression of these genes in different brain regions at various time points in male Sprague - Dawley rats. Using semi quantitativein situ hybridization with 35S-riboprobes to evaluate mRNA levels, the diurnal rhythmicity of rPer1, and rPer2 mRNA levels was found in the SCN, arcuate nucleus, and median eminence/pars tuberalis. Expression patterns of mRNA for rPer1 and rPer2, however, were not similar in these brain regions. The rhythmicity in these brain regions was specific, because it was not observed in the cerebellum or hippocampus. Moreover, diurnal changes in rClock mRNA expression were not detected in any of the brain regions examined. These findings suggest that the different expression patterns observed for rPer1, rPer2, and rClock mRNAs may be attributed to their different physiological roles in these brain regions, and support previous work indicating that circadian rhythms in the brain are widespread.

Original languageEnglish (US)
Pages (from-to)209-217
Number of pages9
JournalJournal of Biomedical Science
Volume12
Issue number1
DOIs
StatePublished - Jan 1 2005
Externally publishedYes

Fingerprint

Rats
Brain
Genes
Messenger RNA
Suprachiasmatic Nucleus
Periodicity
Median Eminence
Arcuate Nucleus of Hypothalamus
Mammals
Circadian Rhythm
Cerebellum
Drosophila
Sprague Dawley Rats
Hippocampus
Gene Expression

Keywords

  • Arcuate nucleus
  • Cerebellum
  • Circadian rhythm
  • Hippocampus
  • Pars tuberalis/median eminence
  • Suprachiasmatic nucleus

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Diurnal rhythmic expression of the rhythm-related genes, rPeriod1, rPeriod2, and rClock, in the rat brain. / Shieh, Kun Ruey; Yang, Shu Chuan; Lu, Xinyun; Akil, Huda; Watson, Stanley J.

In: Journal of Biomedical Science, Vol. 12, No. 1, 01.01.2005, p. 209-217.

Research output: Contribution to journalArticle

Shieh, Kun Ruey ; Yang, Shu Chuan ; Lu, Xinyun ; Akil, Huda ; Watson, Stanley J. / Diurnal rhythmic expression of the rhythm-related genes, rPeriod1, rPeriod2, and rClock, in the rat brain. In: Journal of Biomedical Science. 2005 ; Vol. 12, No. 1. pp. 209-217.
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