DNA alterations in human aberrant crypt foci and colon cancers by random primed polymerase chain reaction

Liping Luo, Biaoru Li, Theresa P. Pretlow

Research output: Contribution to journalArticle

31 Scopus citations


Colon cancers are the result of the accumulation of multiple genetic alterations. To evaluate the role genomic instability plays during tumor development, we compared DNA fingerprints of 44 aberrant crypt foci (ACF; the earliest identified neoplastic lesion in the colon), 23 cancers, and normal crypts generated by random primers with PCR. The PCR products, separated by PAGE and viewed after silver staining, demonstrate altered fingerprints for 23.3% of the ACF and 95.7% of the cancers. In this first study of human ACF with this approach, the finding of altered DNA fingerprints in these microscopic lesions suggests that genomic instability can occur very early in human colon tumorigenesis.

Original languageEnglish (US)
Pages (from-to)6166-6169
Number of pages4
JournalCancer Research
Issue number19
Publication statusPublished - Oct 1 2003
Externally publishedYes


ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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