DNA alterations in human aberrant crypt foci and colon cancers by random primed polymerase chain reaction

Liping Luo, Biaoru Li, Theresa P. Pretlow

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Colon cancers are the result of the accumulation of multiple genetic alterations. To evaluate the role genomic instability plays during tumor development, we compared DNA fingerprints of 44 aberrant crypt foci (ACF; the earliest identified neoplastic lesion in the colon), 23 cancers, and normal crypts generated by random primers with PCR. The PCR products, separated by PAGE and viewed after silver staining, demonstrate altered fingerprints for 23.3% of the ACF and 95.7% of the cancers. In this first study of human ACF with this approach, the finding of altered DNA fingerprints in these microscopic lesions suggests that genomic instability can occur very early in human colon tumorigenesis.

Original languageEnglish (US)
Pages (from-to)6166-6169
Number of pages4
JournalCancer Research
Volume63
Issue number19
StatePublished - Oct 1 2003
Externally publishedYes

Fingerprint

Aberrant Crypt Foci
DNA Fingerprinting
Genomic Instability
Colonic Neoplasms
Polymerase Chain Reaction
Silver Staining
DNA
Dermatoglyphics
Neoplasms
Colon
Carcinogenesis

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

DNA alterations in human aberrant crypt foci and colon cancers by random primed polymerase chain reaction. / Luo, Liping; Li, Biaoru; Pretlow, Theresa P.

In: Cancer Research, Vol. 63, No. 19, 01.10.2003, p. 6166-6169.

Research output: Contribution to journalArticle

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