DNA damage and expression of checkpoint genes p21WAF1/CIP1 and 14-3-3 σ in taurine-deficient cardiomyocytes

Olga Golubnitschaja, Heike Moenkemann, Kerstin Kim, Mahmood S Mozaffari

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Objective: Taurine depletion is associated with development of cardiomyopathy. Further, oxidative stress is advanced as a critical factor mediating the effect of taurine deficiency on target organs. However, the molecular mechanism(s) linking taurine deficiency with the development of cardiomyopathy remains elusive. Since transition between apoptotic degeneration and cell proliferation in stress conditions is regulated at cell cycle checkpoints, we determined the expression of two such genes, namely p21WAF1/CIP1 and 14-3-3 σ as well as p53 that are responsible for oxidative stress and DNA damage. We also carried out quantitative determination of DNA damage. Methods: Cardiomyocytes from β-alanine-induced taurine-depleted (TD) rats were used for this investigation. Single- and double-stranded DNA damage was quantified using comet assay analysis. Western blot and two-dimensional polyacrylamide gel electrophoresis with immunoblotting analysis were applied for protein analysis. Results: Comet assay analysis indicated that the extent of double-stranded DNA damage was greater in TD than in control cardiomyocytes. Whereas only traces of both p53 and p21WAF1/CIP1 and no detectable expression of 14-3-3 σ were found in cardiomyocytes of control animals, the TD cardiomyocytes expressed all three genes. Conclusions: DNA damage and the consequent up-regulation of checkpoint proteins observed in TD cardiomyocytes indicate the involvement of cell cycle control mechanisms in the effect of taurine deficiency on cardiomyocytes. Single- and double-stranded DNA damage and the consequent arrest of cell proliferation in both G1 and G2 phases of the cell cycle induced by checkpoint proteins may trigger the cardiomyopathy that is associated with taurine deficiency.

Original languageEnglish (US)
Pages (from-to)511-517
Number of pages7
JournalBiochemical Pharmacology
Volume66
Issue number3
DOIs
StatePublished - Aug 1 2003

Fingerprint

Taurine
Cardiac Myocytes
DNA Damage
Genes
Gene Expression
DNA
Cardiomyopathies
Oxidative stress
Comet Assay
Single-Stranded DNA
Cells
Cell proliferation
Cell Cycle Checkpoints
Assays
Oxidative Stress
Cell Proliferation
G2 Phase Cell Cycle Checkpoints
Proteins
Electrophoresis, Gel, Two-Dimensional
G1 Phase

Keywords

  • Cardiomyocytes ex vivo
  • Checkpoint proteins
  • Comet assay
  • DNA damage
  • Gene expression
  • Taurine depletion

ASJC Scopus subject areas

  • Biochemistry
  • Pharmacology

Cite this

DNA damage and expression of checkpoint genes p21WAF1/CIP1 and 14-3-3 σ in taurine-deficient cardiomyocytes. / Golubnitschaja, Olga; Moenkemann, Heike; Kim, Kerstin; Mozaffari, Mahmood S.

In: Biochemical Pharmacology, Vol. 66, No. 3, 01.08.2003, p. 511-517.

Research output: Contribution to journalArticle

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abstract = "Objective: Taurine depletion is associated with development of cardiomyopathy. Further, oxidative stress is advanced as a critical factor mediating the effect of taurine deficiency on target organs. However, the molecular mechanism(s) linking taurine deficiency with the development of cardiomyopathy remains elusive. Since transition between apoptotic degeneration and cell proliferation in stress conditions is regulated at cell cycle checkpoints, we determined the expression of two such genes, namely p21WAF1/CIP1 and 14-3-3 σ as well as p53 that are responsible for oxidative stress and DNA damage. We also carried out quantitative determination of DNA damage. Methods: Cardiomyocytes from β-alanine-induced taurine-depleted (TD) rats were used for this investigation. Single- and double-stranded DNA damage was quantified using comet assay analysis. Western blot and two-dimensional polyacrylamide gel electrophoresis with immunoblotting analysis were applied for protein analysis. Results: Comet assay analysis indicated that the extent of double-stranded DNA damage was greater in TD than in control cardiomyocytes. Whereas only traces of both p53 and p21WAF1/CIP1 and no detectable expression of 14-3-3 σ were found in cardiomyocytes of control animals, the TD cardiomyocytes expressed all three genes. Conclusions: DNA damage and the consequent up-regulation of checkpoint proteins observed in TD cardiomyocytes indicate the involvement of cell cycle control mechanisms in the effect of taurine deficiency on cardiomyocytes. Single- and double-stranded DNA damage and the consequent arrest of cell proliferation in both G1 and G2 phases of the cell cycle induced by checkpoint proteins may trigger the cardiomyopathy that is associated with taurine deficiency.",
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AU - Mozaffari, Mahmood S

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N2 - Objective: Taurine depletion is associated with development of cardiomyopathy. Further, oxidative stress is advanced as a critical factor mediating the effect of taurine deficiency on target organs. However, the molecular mechanism(s) linking taurine deficiency with the development of cardiomyopathy remains elusive. Since transition between apoptotic degeneration and cell proliferation in stress conditions is regulated at cell cycle checkpoints, we determined the expression of two such genes, namely p21WAF1/CIP1 and 14-3-3 σ as well as p53 that are responsible for oxidative stress and DNA damage. We also carried out quantitative determination of DNA damage. Methods: Cardiomyocytes from β-alanine-induced taurine-depleted (TD) rats were used for this investigation. Single- and double-stranded DNA damage was quantified using comet assay analysis. Western blot and two-dimensional polyacrylamide gel electrophoresis with immunoblotting analysis were applied for protein analysis. Results: Comet assay analysis indicated that the extent of double-stranded DNA damage was greater in TD than in control cardiomyocytes. Whereas only traces of both p53 and p21WAF1/CIP1 and no detectable expression of 14-3-3 σ were found in cardiomyocytes of control animals, the TD cardiomyocytes expressed all three genes. Conclusions: DNA damage and the consequent up-regulation of checkpoint proteins observed in TD cardiomyocytes indicate the involvement of cell cycle control mechanisms in the effect of taurine deficiency on cardiomyocytes. Single- and double-stranded DNA damage and the consequent arrest of cell proliferation in both G1 and G2 phases of the cell cycle induced by checkpoint proteins may trigger the cardiomyopathy that is associated with taurine deficiency.

AB - Objective: Taurine depletion is associated with development of cardiomyopathy. Further, oxidative stress is advanced as a critical factor mediating the effect of taurine deficiency on target organs. However, the molecular mechanism(s) linking taurine deficiency with the development of cardiomyopathy remains elusive. Since transition between apoptotic degeneration and cell proliferation in stress conditions is regulated at cell cycle checkpoints, we determined the expression of two such genes, namely p21WAF1/CIP1 and 14-3-3 σ as well as p53 that are responsible for oxidative stress and DNA damage. We also carried out quantitative determination of DNA damage. Methods: Cardiomyocytes from β-alanine-induced taurine-depleted (TD) rats were used for this investigation. Single- and double-stranded DNA damage was quantified using comet assay analysis. Western blot and two-dimensional polyacrylamide gel electrophoresis with immunoblotting analysis were applied for protein analysis. Results: Comet assay analysis indicated that the extent of double-stranded DNA damage was greater in TD than in control cardiomyocytes. Whereas only traces of both p53 and p21WAF1/CIP1 and no detectable expression of 14-3-3 σ were found in cardiomyocytes of control animals, the TD cardiomyocytes expressed all three genes. Conclusions: DNA damage and the consequent up-regulation of checkpoint proteins observed in TD cardiomyocytes indicate the involvement of cell cycle control mechanisms in the effect of taurine deficiency on cardiomyocytes. Single- and double-stranded DNA damage and the consequent arrest of cell proliferation in both G1 and G2 phases of the cell cycle induced by checkpoint proteins may trigger the cardiomyopathy that is associated with taurine deficiency.

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