DNA demethylation in retinal neurocytes contributes to the upregulation of DNA repair protein, Ku80

Jing Zhuang, Yiming Ye, Xuan Liu, Fan Li, Xueke Pan, Zhao Chen, Huihui Luo, Yihong Ge, Jian Ge, Joseph Michael Kaminski, Keming Yu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Ku80 plays a critical role in DNA double strand breaks repair. However, Ku80 is silenced in mature neurocytes. In this study, the mechanism of Ku80 silencing and its role in DNA double strand break repair in retinal neurocytes was investigated. Our data show that Ku80 expression is activated in primary cultured retinal neurocytes after treatment with 5-azacytidine in vitro, whereas methylation of -179 bp in Ku80 promoter induces Ku80 silencing in retinal neurocytes. Ku80 reactivation in retinal neurocytes by 5-azacytidine enhances DNA integrity after treatment with H2O2. Therefore, our data suggest Ku80 might be a target for reactivation to increase retinal neuronal DNA repair.

Original languageEnglish (US)
Pages (from-to)282-286
Number of pages5
JournalNeuroReport
Volume21
Issue number4
DOIs
StatePublished - Mar 1 2010

Fingerprint

Azacitidine
Double-Stranded DNA Breaks
DNA Repair
Up-Regulation
DNA
Methylation
Proteins
In Vitro Techniques

Keywords

  • DNA repair
  • Ku80
  • Methylation
  • Nonhomologues end joining

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

DNA demethylation in retinal neurocytes contributes to the upregulation of DNA repair protein, Ku80. / Zhuang, Jing; Ye, Yiming; Liu, Xuan; Li, Fan; Pan, Xueke; Chen, Zhao; Luo, Huihui; Ge, Yihong; Ge, Jian; Kaminski, Joseph Michael; Yu, Keming.

In: NeuroReport, Vol. 21, No. 4, 01.03.2010, p. 282-286.

Research output: Contribution to journalArticle

Zhuang, J, Ye, Y, Liu, X, Li, F, Pan, X, Chen, Z, Luo, H, Ge, Y, Ge, J, Kaminski, JM & Yu, K 2010, 'DNA demethylation in retinal neurocytes contributes to the upregulation of DNA repair protein, Ku80', NeuroReport, vol. 21, no. 4, pp. 282-286. https://doi.org/10.1097/WNR.0b013e328336ee7e
Zhuang, Jing ; Ye, Yiming ; Liu, Xuan ; Li, Fan ; Pan, Xueke ; Chen, Zhao ; Luo, Huihui ; Ge, Yihong ; Ge, Jian ; Kaminski, Joseph Michael ; Yu, Keming. / DNA demethylation in retinal neurocytes contributes to the upregulation of DNA repair protein, Ku80. In: NeuroReport. 2010 ; Vol. 21, No. 4. pp. 282-286.
@article{ad003ec6eb5f471d8335574274bbcece,
title = "DNA demethylation in retinal neurocytes contributes to the upregulation of DNA repair protein, Ku80",
abstract = "Ku80 plays a critical role in DNA double strand breaks repair. However, Ku80 is silenced in mature neurocytes. In this study, the mechanism of Ku80 silencing and its role in DNA double strand break repair in retinal neurocytes was investigated. Our data show that Ku80 expression is activated in primary cultured retinal neurocytes after treatment with 5-azacytidine in vitro, whereas methylation of -179 bp in Ku80 promoter induces Ku80 silencing in retinal neurocytes. Ku80 reactivation in retinal neurocytes by 5-azacytidine enhances DNA integrity after treatment with H2O2. Therefore, our data suggest Ku80 might be a target for reactivation to increase retinal neuronal DNA repair.",
keywords = "DNA repair, Ku80, Methylation, Nonhomologues end joining",
author = "Jing Zhuang and Yiming Ye and Xuan Liu and Fan Li and Xueke Pan and Zhao Chen and Huihui Luo and Yihong Ge and Jian Ge and Kaminski, {Joseph Michael} and Keming Yu",
year = "2010",
month = "3",
day = "1",
doi = "10.1097/WNR.0b013e328336ee7e",
language = "English (US)",
volume = "21",
pages = "282--286",
journal = "NeuroReport",
issn = "0959-4965",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

TY - JOUR

T1 - DNA demethylation in retinal neurocytes contributes to the upregulation of DNA repair protein, Ku80

AU - Zhuang, Jing

AU - Ye, Yiming

AU - Liu, Xuan

AU - Li, Fan

AU - Pan, Xueke

AU - Chen, Zhao

AU - Luo, Huihui

AU - Ge, Yihong

AU - Ge, Jian

AU - Kaminski, Joseph Michael

AU - Yu, Keming

PY - 2010/3/1

Y1 - 2010/3/1

N2 - Ku80 plays a critical role in DNA double strand breaks repair. However, Ku80 is silenced in mature neurocytes. In this study, the mechanism of Ku80 silencing and its role in DNA double strand break repair in retinal neurocytes was investigated. Our data show that Ku80 expression is activated in primary cultured retinal neurocytes after treatment with 5-azacytidine in vitro, whereas methylation of -179 bp in Ku80 promoter induces Ku80 silencing in retinal neurocytes. Ku80 reactivation in retinal neurocytes by 5-azacytidine enhances DNA integrity after treatment with H2O2. Therefore, our data suggest Ku80 might be a target for reactivation to increase retinal neuronal DNA repair.

AB - Ku80 plays a critical role in DNA double strand breaks repair. However, Ku80 is silenced in mature neurocytes. In this study, the mechanism of Ku80 silencing and its role in DNA double strand break repair in retinal neurocytes was investigated. Our data show that Ku80 expression is activated in primary cultured retinal neurocytes after treatment with 5-azacytidine in vitro, whereas methylation of -179 bp in Ku80 promoter induces Ku80 silencing in retinal neurocytes. Ku80 reactivation in retinal neurocytes by 5-azacytidine enhances DNA integrity after treatment with H2O2. Therefore, our data suggest Ku80 might be a target for reactivation to increase retinal neuronal DNA repair.

KW - DNA repair

KW - Ku80

KW - Methylation

KW - Nonhomologues end joining

UR - http://www.scopus.com/inward/record.url?scp=76849087250&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=76849087250&partnerID=8YFLogxK

U2 - 10.1097/WNR.0b013e328336ee7e

DO - 10.1097/WNR.0b013e328336ee7e

M3 - Article

C2 - 20145501

AN - SCOPUS:76849087250

VL - 21

SP - 282

EP - 286

JO - NeuroReport

JF - NeuroReport

SN - 0959-4965

IS - 4

ER -