DNA epitope vaccine containing complement component C3d enhances anti-amyloid-β antibody production and polarizes the immune response towards a Th2 phenotype

Nina Movsesyan, Mikayel Mkrtichyan, Irina Petrushina, Ted M. Ross, David H. Cribbs, Michael G. Agadjanyan, Anahit Ghochikyan

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We have engineered a DNA epitope vaccine that expresses 3 self-B cell epitopes of Aβ42 (3Aβ1-11), a non-self T helper (Th) cell epitope (PADRE), and 3 copies of C3d (3C3d), a component of complement as a molecular adjuvant, designed to safely reduce CNS Aβ. Immunization of mice with 3Aβ1-11-PADRE epitope vaccine alone generated only moderate levels of anti-Aβ antibodies and a pro-inflammatory T helper (Th1 phenotype) cellular immune response. However, the addition of 3C3d to the vaccine construct significantly augmented the anti-Aβ humoral immune response and, importantly, shifted the cellular immune response towards the potentially safer anti-inflammatory Th2 phenotype.

Original languageEnglish (US)
Pages (from-to)57-63
Number of pages7
JournalJournal of Neuroimmunology
Volume205
Issue number1-2
DOIs
StatePublished - Dec 15 2008

Fingerprint

Complement C3d
DNA Vaccines
Amyloid
Cellular Immunity
Antibody Formation
Epitopes
Anti-Idiotypic Antibodies
Vaccines
B-Lymphocyte Epitopes
Phenotype
T-Lymphocyte Epitopes
Humoral Immunity
Immunization
Anti-Inflammatory Agents

Keywords

  • AN-1792
  • Alzheimer's disease
  • Amyloid-beta
  • DNA vaccine
  • Immunotherapy
  • Molecular adjuvant

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Medicine(all)
  • Neurology
  • Clinical Neurology

Cite this

DNA epitope vaccine containing complement component C3d enhances anti-amyloid-β antibody production and polarizes the immune response towards a Th2 phenotype. / Movsesyan, Nina; Mkrtichyan, Mikayel; Petrushina, Irina; Ross, Ted M.; Cribbs, David H.; Agadjanyan, Michael G.; Ghochikyan, Anahit.

In: Journal of Neuroimmunology, Vol. 205, No. 1-2, 15.12.2008, p. 57-63.

Research output: Contribution to journalArticle

Movsesyan, Nina ; Mkrtichyan, Mikayel ; Petrushina, Irina ; Ross, Ted M. ; Cribbs, David H. ; Agadjanyan, Michael G. ; Ghochikyan, Anahit. / DNA epitope vaccine containing complement component C3d enhances anti-amyloid-β antibody production and polarizes the immune response towards a Th2 phenotype. In: Journal of Neuroimmunology. 2008 ; Vol. 205, No. 1-2. pp. 57-63.
@article{2dae6a37d6f5477eb6fb3e2d72a8b6bf,
title = "DNA epitope vaccine containing complement component C3d enhances anti-amyloid-β antibody production and polarizes the immune response towards a Th2 phenotype",
abstract = "We have engineered a DNA epitope vaccine that expresses 3 self-B cell epitopes of Aβ42 (3Aβ1-11), a non-self T helper (Th) cell epitope (PADRE), and 3 copies of C3d (3C3d), a component of complement as a molecular adjuvant, designed to safely reduce CNS Aβ. Immunization of mice with 3Aβ1-11-PADRE epitope vaccine alone generated only moderate levels of anti-Aβ antibodies and a pro-inflammatory T helper (Th1 phenotype) cellular immune response. However, the addition of 3C3d to the vaccine construct significantly augmented the anti-Aβ humoral immune response and, importantly, shifted the cellular immune response towards the potentially safer anti-inflammatory Th2 phenotype.",
keywords = "AN-1792, Alzheimer's disease, Amyloid-beta, DNA vaccine, Immunotherapy, Molecular adjuvant",
author = "Nina Movsesyan and Mikayel Mkrtichyan and Irina Petrushina and Ross, {Ted M.} and Cribbs, {David H.} and Agadjanyan, {Michael G.} and Anahit Ghochikyan",
year = "2008",
month = "12",
day = "15",
doi = "10.1016/j.jneuroim.2008.08.016",
language = "English (US)",
volume = "205",
pages = "57--63",
journal = "Journal of Neuroimmunology",
issn = "0165-5728",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - DNA epitope vaccine containing complement component C3d enhances anti-amyloid-β antibody production and polarizes the immune response towards a Th2 phenotype

AU - Movsesyan, Nina

AU - Mkrtichyan, Mikayel

AU - Petrushina, Irina

AU - Ross, Ted M.

AU - Cribbs, David H.

AU - Agadjanyan, Michael G.

AU - Ghochikyan, Anahit

PY - 2008/12/15

Y1 - 2008/12/15

N2 - We have engineered a DNA epitope vaccine that expresses 3 self-B cell epitopes of Aβ42 (3Aβ1-11), a non-self T helper (Th) cell epitope (PADRE), and 3 copies of C3d (3C3d), a component of complement as a molecular adjuvant, designed to safely reduce CNS Aβ. Immunization of mice with 3Aβ1-11-PADRE epitope vaccine alone generated only moderate levels of anti-Aβ antibodies and a pro-inflammatory T helper (Th1 phenotype) cellular immune response. However, the addition of 3C3d to the vaccine construct significantly augmented the anti-Aβ humoral immune response and, importantly, shifted the cellular immune response towards the potentially safer anti-inflammatory Th2 phenotype.

AB - We have engineered a DNA epitope vaccine that expresses 3 self-B cell epitopes of Aβ42 (3Aβ1-11), a non-self T helper (Th) cell epitope (PADRE), and 3 copies of C3d (3C3d), a component of complement as a molecular adjuvant, designed to safely reduce CNS Aβ. Immunization of mice with 3Aβ1-11-PADRE epitope vaccine alone generated only moderate levels of anti-Aβ antibodies and a pro-inflammatory T helper (Th1 phenotype) cellular immune response. However, the addition of 3C3d to the vaccine construct significantly augmented the anti-Aβ humoral immune response and, importantly, shifted the cellular immune response towards the potentially safer anti-inflammatory Th2 phenotype.

KW - AN-1792

KW - Alzheimer's disease

KW - Amyloid-beta

KW - DNA vaccine

KW - Immunotherapy

KW - Molecular adjuvant

UR - http://www.scopus.com/inward/record.url?scp=56349165547&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=56349165547&partnerID=8YFLogxK

U2 - 10.1016/j.jneuroim.2008.08.016

DO - 10.1016/j.jneuroim.2008.08.016

M3 - Article

VL - 205

SP - 57

EP - 63

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

SN - 0165-5728

IS - 1-2

ER -