DNK iz raznykh organov dal'nevostochnykh listvennits i ee prigodnost' dlia RAPD-analiza.

Translated title of the contribution: DNA from various tissues of the Far Eastern larch and its applicability for RAPD assay

Irina Yurievna Sazonova, M. M. Kozyrenko, E. V. Artiukova, G. D. Reunova, I. N. Zhuravlev

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

We studied applicability of RAPD assay for investigating genetic variability of far eastern larches. Techniques to isolate DNA from larch vegetative organs (needles, young shoots and stems) and megagametophytes were selected, and conditions of polymerase chain reaction were optimized. Fifty out of 84 random primers proved efficient, and 22 and 12 primers initiated weak and no synthesis, respectively. The tested primers worked efficiently on the DNA isolated from both diploid and haploid tissues.

Translated title of the contributionDNA from various tissues of the Far Eastern larch and its applicability for RAPD assay
Original languageRussian
Pages (from-to)243-248
Number of pages6
JournalIzvestiia Akademii nauk. Seriia biologicheskaia / Rossiiskaia akademiia nauk
Issue number2
StatePublished - Jan 1 2001

ASJC Scopus subject areas

  • Medicine(all)

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    Sazonova, I. Y., Kozyrenko, M. M., Artiukova, E. V., Reunova, G. D., & Zhuravlev, I. N. (2001). DNK iz raznykh organov dal'nevostochnykh listvennits i ee prigodnost' dlia RAPD-analiza. Izvestiia Akademii nauk. Seriia biologicheskaia / Rossiiskaia akademiia nauk, (2), 243-248.