DNA Photoreactivating Enzyme from Placental Mammals. Origin and Characteristics

Betsy M. Sutherland, Paul Runge, John C. Sutherland

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

DNA photoreactivating enzyme in human leukocytes is concentrated in the phagocytotic monocytes and polymorphonuclear cells. Lymphocytes, erythrocytes, spleen, and serum contain little if any enzyme activity. Since bone marrow, which contains immature, nonphagocytotic monocytes and polymorphonuclear cells, also contains high levels of enzyme, it is unlikely that the enzyme in the mature cells resulted from bacteria engulfed by these cells. Photoreactivating enzyme is also found in murine, and in human cells in culture; confluent murine cells have higher specific activity than do rapidly growing cells. The leukocyte enzyme, which requires ultraviolet-irradiated DNA as substrate and visible light for catalysis, converts pyrimidine dimers in DNA to the corresponding monomers in the lightdependent reaction. The action spectrum for photoreactivation extends from 300 to 600 nm, with a peak at about 400 nm.

Original languageEnglish (US)
Pages (from-to)4710-4715
Number of pages6
JournalBiochemistry
Volume13
Issue number23
DOIs
StatePublished - Nov 1 1974
Externally publishedYes

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Deoxyribodipyrimidine Photo-Lyase
Mammals
Enzymes
Pyrimidine Dimers
Lymphocytes
DNA
Enzyme activity
Cell culture
Catalysis
Monocytes
Bacteria
Bone
Leukocytes
Monomers
Cells
Substrates
Spleen
Cell Culture Techniques
Erythrocytes
Bone Marrow

ASJC Scopus subject areas

  • Biochemistry

Cite this

DNA Photoreactivating Enzyme from Placental Mammals. Origin and Characteristics. / Sutherland, Betsy M.; Runge, Paul; Sutherland, John C.

In: Biochemistry, Vol. 13, No. 23, 01.11.1974, p. 4710-4715.

Research output: Contribution to journalArticle

Sutherland, Betsy M. ; Runge, Paul ; Sutherland, John C. / DNA Photoreactivating Enzyme from Placental Mammals. Origin and Characteristics. In: Biochemistry. 1974 ; Vol. 13, No. 23. pp. 4710-4715.
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