DNA polymorphism of leptin gene in Bos indicus and Bos taurus cattle

Vivek Choudhary, Pushpendra Kumar, Tarun K. Bhattacharya, Bharat Bhushan, Arjava Sharma

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Leptin plays an important role in the regulation of feed intake, energy metabolism, growth and reproduction of cattle. We used the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique to screen for DNA polymorphisms of the leptin gene in 403 cattle belonging to various breeds of Bos indicus (Hariana, Sahiwal, Gir and Nimari cattle), Bos taurus (Holstein Friesian (HF) and Jersey cattle) as well as Bos taurus x Bos indicus crossbreds (1/2 HF × 1 /2 Hariana). In all the cattle we amplified two regions of the leptin gene, a 522 bp fragment comprising the partial intron 2 and exon 3 and another 94 bp fragment consisting of part of exon 2. Digestion of 522 bp PCR products with the BsaAl restriction enzyme revealed three genotypes in all the breeds of cattle studied. This is the first report of the presence of leptin gene polymorphism in purebred Bos indicus cattle of Indian origin (indicine cattle). Almost similar gene and genotype frequencies were observed in all the breed groups, while the frequency of mutant homozygotes (AA) was very low (0.03 to 0.07). On digestion of the 94 bp fragment with the Kpn2I restriction enzyme, three genotypes were observed in HF, Jersey and crossbred cattle. The CC genotype had the highest frequency (0.68) in crossbreds whereas the frequency of CT heterozygotes was highest (0.69) in HF cattle. This mutation was absent in all the breeds of indicine cattle. The results suggest that the BsaAl-RFLP mutation has occurred far back in evolution before the divergence of taurine and indicine cattle while the Kpn2I mutation has occurred recently as indicated by the fact that this mutation was only detected in taurine cattle. Copyright by the Brazilian Society of Genetics.

Original languageEnglish (US)
Pages (from-to)740-742
Number of pages3
JournalGenetics and Molecular Biology
Volume28
Issue number4
DOIs
StatePublished - Jan 1 2005
Externally publishedYes

Fingerprint

Leptin
DNA
Genes
Genotype
Mutation
Taurine
Restriction Fragment Length Polymorphisms
Digestion
Exons
Polymerase Chain Reaction
Homozygote
Enzymes
Heterozygote
Gene Frequency
Introns
Energy Metabolism

Keywords

  • Bos indicus
  • Bos taurus
  • Leptin gene
  • PCR-RFLP
  • Polymorphism

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

DNA polymorphism of leptin gene in Bos indicus and Bos taurus cattle. / Choudhary, Vivek; Kumar, Pushpendra; Bhattacharya, Tarun K.; Bhushan, Bharat; Sharma, Arjava.

In: Genetics and Molecular Biology, Vol. 28, No. 4, 01.01.2005, p. 740-742.

Research output: Contribution to journalArticle

Choudhary, Vivek ; Kumar, Pushpendra ; Bhattacharya, Tarun K. ; Bhushan, Bharat ; Sharma, Arjava. / DNA polymorphism of leptin gene in Bos indicus and Bos taurus cattle. In: Genetics and Molecular Biology. 2005 ; Vol. 28, No. 4. pp. 740-742.
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