Dog introduction alters the home dust microbiota

A. R. Sitarik, S. Havstad, A. M. Levin, S. V. Lynch, K. E. Fujimura, Dennis Randall Ownby, C. C. Johnson, G. Wegienka

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Research has largely reported that dog exposure is associated with reduced allergic disease risk. Responsible mechanism(s) are not understood. The goal was to investigate whether introducing a dog into the home changes the home dust microbiota. Families without dogs or cats planning to adopt a dog and those who were not were recruited. Dust samples were collected from the homes at recruitment and 12 months later. Microbiota composition and taxa (V4 region of the 16S rRNA gene) were compared between homes that did and did not adopt a dog. A total of 91 dust samples from 54 families (27 each, dog and no dog; 17 dog and 20 no dog homes with paired samples) were analyzed. A significant dog effect was seen across time in both unweighted UniFrac and Canberra metrics (both P =.008), indicating dog introduction may result in rapid establishment of rarer and phylogenetically related taxa. A significant dog-time interaction was seen in both weighted UniFrac (P <.001) and Bray-Curtis (P =.002) metrics, suggesting that while there may not initially be large relative abundance shifts following dog introduction, differences can be seen within a year. Therefore, dog introduction into the home has both immediate effects and effects that emerge over time.

Original languageEnglish (US)
Pages (from-to)539-547
Number of pages9
JournalIndoor Air
Volume28
Issue number4
DOIs
StatePublished - Jul 1 2018

Fingerprint

Microbiota
Dust
Dogs
Genes
Planning
Chemical analysis
rRNA Genes
Cats

Keywords

  • allergy
  • asthma
  • dog
  • hygiene hypothesis
  • microbiome
  • microbiota hypothesis

ASJC Scopus subject areas

  • Environmental Engineering
  • Building and Construction
  • Public Health, Environmental and Occupational Health

Cite this

Sitarik, A. R., Havstad, S., Levin, A. M., Lynch, S. V., Fujimura, K. E., Ownby, D. R., ... Wegienka, G. (2018). Dog introduction alters the home dust microbiota. Indoor Air, 28(4), 539-547. https://doi.org/10.1111/ina.12456

Dog introduction alters the home dust microbiota. / Sitarik, A. R.; Havstad, S.; Levin, A. M.; Lynch, S. V.; Fujimura, K. E.; Ownby, Dennis Randall; Johnson, C. C.; Wegienka, G.

In: Indoor Air, Vol. 28, No. 4, 01.07.2018, p. 539-547.

Research output: Contribution to journalArticle

Sitarik, AR, Havstad, S, Levin, AM, Lynch, SV, Fujimura, KE, Ownby, DR, Johnson, CC & Wegienka, G 2018, 'Dog introduction alters the home dust microbiota', Indoor Air, vol. 28, no. 4, pp. 539-547. https://doi.org/10.1111/ina.12456
Sitarik AR, Havstad S, Levin AM, Lynch SV, Fujimura KE, Ownby DR et al. Dog introduction alters the home dust microbiota. Indoor Air. 2018 Jul 1;28(4):539-547. https://doi.org/10.1111/ina.12456
Sitarik, A. R. ; Havstad, S. ; Levin, A. M. ; Lynch, S. V. ; Fujimura, K. E. ; Ownby, Dennis Randall ; Johnson, C. C. ; Wegienka, G. / Dog introduction alters the home dust microbiota. In: Indoor Air. 2018 ; Vol. 28, No. 4. pp. 539-547.
@article{4194a109e57645998c748f7c9629cbbc,
title = "Dog introduction alters the home dust microbiota",
abstract = "Research has largely reported that dog exposure is associated with reduced allergic disease risk. Responsible mechanism(s) are not understood. The goal was to investigate whether introducing a dog into the home changes the home dust microbiota. Families without dogs or cats planning to adopt a dog and those who were not were recruited. Dust samples were collected from the homes at recruitment and 12 months later. Microbiota composition and taxa (V4 region of the 16S rRNA gene) were compared between homes that did and did not adopt a dog. A total of 91 dust samples from 54 families (27 each, dog and no dog; 17 dog and 20 no dog homes with paired samples) were analyzed. A significant dog effect was seen across time in both unweighted UniFrac and Canberra metrics (both P =.008), indicating dog introduction may result in rapid establishment of rarer and phylogenetically related taxa. A significant dog-time interaction was seen in both weighted UniFrac (P <.001) and Bray-Curtis (P =.002) metrics, suggesting that while there may not initially be large relative abundance shifts following dog introduction, differences can be seen within a year. Therefore, dog introduction into the home has both immediate effects and effects that emerge over time.",
keywords = "allergy, asthma, dog, hygiene hypothesis, microbiome, microbiota hypothesis",
author = "Sitarik, {A. R.} and S. Havstad and Levin, {A. M.} and Lynch, {S. V.} and Fujimura, {K. E.} and Ownby, {Dennis Randall} and Johnson, {C. C.} and G. Wegienka",
year = "2018",
month = "7",
day = "1",
doi = "10.1111/ina.12456",
language = "English (US)",
volume = "28",
pages = "539--547",
journal = "Indoor Air",
issn = "0905-6947",
publisher = "Blackwell Munksgaard",
number = "4",

}

TY - JOUR

T1 - Dog introduction alters the home dust microbiota

AU - Sitarik, A. R.

AU - Havstad, S.

AU - Levin, A. M.

AU - Lynch, S. V.

AU - Fujimura, K. E.

AU - Ownby, Dennis Randall

AU - Johnson, C. C.

AU - Wegienka, G.

PY - 2018/7/1

Y1 - 2018/7/1

N2 - Research has largely reported that dog exposure is associated with reduced allergic disease risk. Responsible mechanism(s) are not understood. The goal was to investigate whether introducing a dog into the home changes the home dust microbiota. Families without dogs or cats planning to adopt a dog and those who were not were recruited. Dust samples were collected from the homes at recruitment and 12 months later. Microbiota composition and taxa (V4 region of the 16S rRNA gene) were compared between homes that did and did not adopt a dog. A total of 91 dust samples from 54 families (27 each, dog and no dog; 17 dog and 20 no dog homes with paired samples) were analyzed. A significant dog effect was seen across time in both unweighted UniFrac and Canberra metrics (both P =.008), indicating dog introduction may result in rapid establishment of rarer and phylogenetically related taxa. A significant dog-time interaction was seen in both weighted UniFrac (P <.001) and Bray-Curtis (P =.002) metrics, suggesting that while there may not initially be large relative abundance shifts following dog introduction, differences can be seen within a year. Therefore, dog introduction into the home has both immediate effects and effects that emerge over time.

AB - Research has largely reported that dog exposure is associated with reduced allergic disease risk. Responsible mechanism(s) are not understood. The goal was to investigate whether introducing a dog into the home changes the home dust microbiota. Families without dogs or cats planning to adopt a dog and those who were not were recruited. Dust samples were collected from the homes at recruitment and 12 months later. Microbiota composition and taxa (V4 region of the 16S rRNA gene) were compared between homes that did and did not adopt a dog. A total of 91 dust samples from 54 families (27 each, dog and no dog; 17 dog and 20 no dog homes with paired samples) were analyzed. A significant dog effect was seen across time in both unweighted UniFrac and Canberra metrics (both P =.008), indicating dog introduction may result in rapid establishment of rarer and phylogenetically related taxa. A significant dog-time interaction was seen in both weighted UniFrac (P <.001) and Bray-Curtis (P =.002) metrics, suggesting that while there may not initially be large relative abundance shifts following dog introduction, differences can be seen within a year. Therefore, dog introduction into the home has both immediate effects and effects that emerge over time.

KW - allergy

KW - asthma

KW - dog

KW - hygiene hypothesis

KW - microbiome

KW - microbiota hypothesis

UR - http://www.scopus.com/inward/record.url?scp=85043586344&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85043586344&partnerID=8YFLogxK

U2 - 10.1111/ina.12456

DO - 10.1111/ina.12456

M3 - Article

C2 - 29468742

AN - SCOPUS:85043586344

VL - 28

SP - 539

EP - 547

JO - Indoor Air

JF - Indoor Air

SN - 0905-6947

IS - 4

ER -