Dual role of NF-κB in apoptosis of THP-1 cells during treatment with etoposide and lipopolysaccharide

Keiko Sato; Takahiro Taniguchi; Maiko Suzuki; Fumiaki Shinohara; Haruhiko Takada; Hidemi Rikiishi

doi:10.1016/S0145-2126(03)00127-9

Dual role of NF-κB in apoptosis of THP-1 cells during treatment with etoposide and lipopolysaccharide

Keiko Sato, Takahiro Taniguchi, Maiko Suzuki, Fumiaki Shinohara, Haruhiko Takada, Hidemi Rikiishi

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

One of the mechanisms repressing apoptosis in tumor cells can involve the expression of anti-apoptotic NF-kappaB target genes. In this study, we demonstrated that a potent NF-kappaB inhibitor, Nalpha-tosyl-L-lysinyl chloromethyl ketone (TLCK), inhibits apoptosis of THP-1 cells triggered by etoposide (VP16), and actinomycin D (ACT D) or cycloheximide inhibits apoptosis. However, persistent activation of NF-kappaB by lipopolysaccharide (LPS) led to the survival of leukemic cells against VP16-induced apoptosis. Thus, the molecular events (Bax/X-chromosome-linked IAP (XIAP)) occurring downstream of NF-kappaB activation during VP16 and/or LPS stimulation may become important to understand the multiple effects of NF-kappaB.

Original language	English (US)
Pages (from-to)	63-69
Number of pages	7
Journal	Leukemia Research
Volume	28
Issue number	1
DOIs	https://doi.org/10.1016/S0145-2126(03)00127-9 https://doi.org/10.1016/s0145-2126(03)00127-9
State	Published - Jan 2004
Externally published	Yes

Keywords

Apoptosis
Bax
Etoposide
Lipopolysaccharide
NF-κB
THP-1 cells
XIAP

ASJC Scopus subject areas

Hematology
Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Dual role of NF-κB in apoptosis of THP-1 cells during treatment with etoposide and lipopolysaccharide",

abstract = "One of the mechanisms repressing apoptosis in tumor cells can involve the expression of anti-apoptotic NF-kappaB target genes. In this study, we demonstrated that a potent NF-kappaB inhibitor, Nalpha-tosyl-L-lysinyl chloromethyl ketone (TLCK), inhibits apoptosis of THP-1 cells triggered by etoposide (VP16), and actinomycin D (ACT D) or cycloheximide inhibits apoptosis. However, persistent activation of NF-kappaB by lipopolysaccharide (LPS) led to the survival of leukemic cells against VP16-induced apoptosis. Thus, the molecular events (Bax/X-chromosome-linked IAP (XIAP)) occurring downstream of NF-kappaB activation during VP16 and/or LPS stimulation may become important to understand the multiple effects of NF-kappaB.",

keywords = "Apoptosis, Bax, Etoposide, Lipopolysaccharide, NF-κB, THP-1 cells, XIAP",

author = "Keiko Sato and Takahiro Taniguchi and Maiko Suzuki and Fumiaki Shinohara and Haruhiko Takada and Hidemi Rikiishi",

note = "Funding Information: This work was supported in part by a Grant-in-Aid for Scientific Research 14571726 from the Japan Society for the Promotion of Science. We thank Mr. D. Mrozek for English editing of the manuscript, and Ms. Y. Togashi for editorial assistance. ",

year = "2004",

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doi = "10.1016/S0145-2126(03)00127-9",

language = "English (US)",

volume = "28",

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journal = "Leukemia Research",

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T1 - Dual role of NF-κB in apoptosis of THP-1 cells during treatment with etoposide and lipopolysaccharide

AU - Sato, Keiko

AU - Taniguchi, Takahiro

AU - Suzuki, Maiko

AU - Shinohara, Fumiaki

AU - Takada, Haruhiko

AU - Rikiishi, Hidemi

N1 - Funding Information: This work was supported in part by a Grant-in-Aid for Scientific Research 14571726 from the Japan Society for the Promotion of Science. We thank Mr. D. Mrozek for English editing of the manuscript, and Ms. Y. Togashi for editorial assistance.

PY - 2004/1

Y1 - 2004/1

N2 - One of the mechanisms repressing apoptosis in tumor cells can involve the expression of anti-apoptotic NF-kappaB target genes. In this study, we demonstrated that a potent NF-kappaB inhibitor, Nalpha-tosyl-L-lysinyl chloromethyl ketone (TLCK), inhibits apoptosis of THP-1 cells triggered by etoposide (VP16), and actinomycin D (ACT D) or cycloheximide inhibits apoptosis. However, persistent activation of NF-kappaB by lipopolysaccharide (LPS) led to the survival of leukemic cells against VP16-induced apoptosis. Thus, the molecular events (Bax/X-chromosome-linked IAP (XIAP)) occurring downstream of NF-kappaB activation during VP16 and/or LPS stimulation may become important to understand the multiple effects of NF-kappaB.

AB - One of the mechanisms repressing apoptosis in tumor cells can involve the expression of anti-apoptotic NF-kappaB target genes. In this study, we demonstrated that a potent NF-kappaB inhibitor, Nalpha-tosyl-L-lysinyl chloromethyl ketone (TLCK), inhibits apoptosis of THP-1 cells triggered by etoposide (VP16), and actinomycin D (ACT D) or cycloheximide inhibits apoptosis. However, persistent activation of NF-kappaB by lipopolysaccharide (LPS) led to the survival of leukemic cells against VP16-induced apoptosis. Thus, the molecular events (Bax/X-chromosome-linked IAP (XIAP)) occurring downstream of NF-kappaB activation during VP16 and/or LPS stimulation may become important to understand the multiple effects of NF-kappaB.

KW - Apoptosis

KW - Bax

KW - Etoposide

KW - Lipopolysaccharide

KW - NF-κB

KW - THP-1 cells

KW - XIAP

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DO - 10.1016/S0145-2126(03)00127-9

M3 - Article

C2 - 14630082

AN - SCOPUS:0242468282

SN - 0145-2126

VL - 28

SP - 63

EP - 69

JO - Leukemia Research

JF - Leukemia Research

IS - 1

ER -

Dual role of NF-κB in apoptosis of THP-1 cells during treatment with etoposide and lipopolysaccharide

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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