Dual role of NF-κB in apoptosis of THP-1 cells during treatment with etoposide and lipopolysaccharide

Keiko Sato, Takahiro Taniguchi, Maiko Suzuki, Fumiaki Shinohara, Haruhiko Takada, Hidemi Rikiishi

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

One of the mechanisms repressing apoptosis in tumor cells can involve the expression of anti-apoptotic NF-kappaB target genes. In this study, we demonstrated that a potent NF-kappaB inhibitor, Nalpha-tosyl-L-lysinyl chloromethyl ketone (TLCK), inhibits apoptosis of THP-1 cells triggered by etoposide (VP16), and actinomycin D (ACT D) or cycloheximide inhibits apoptosis. However, persistent activation of NF-kappaB by lipopolysaccharide (LPS) led to the survival of leukemic cells against VP16-induced apoptosis. Thus, the molecular events (Bax/X-chromosome-linked IAP (XIAP)) occurring downstream of NF-kappaB activation during VP16 and/or LPS stimulation may become important to understand the multiple effects of NF-kappaB.
Original languageEnglish (US)
Pages (from-to)63-69
Number of pages7
JournalLeukemia Research
Volume28
Issue number1
DOIs
StatePublished - Jan 2004
Externally publishedYes

Keywords

  • Apoptosis
  • Bax
  • Etoposide
  • Lipopolysaccharide
  • NF-κB
  • THP-1 cells
  • XIAP

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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