Abstract
The effects of ouabain and extracellular calcium removal on gap junctional coupling of isolated rabbit corneal endothelium was examined using a modified dye-spread technique. This technique is a modification of a microelectrode procedure that now utilizes patch electrodes connected to a current-clamp circuit for dye iontophoresis and a shuttering system in the excitation light path to reduce phototoxic effects in the monolayer. It was found that a significant degree of junctional uncoupling occurred after 45 min of exposure to ouabain, quantified as a reduction in the effective diffusion coefficient of injected Lucifer yellow CH: 1.74×10-7 cm2/s (control) versus 0.43×10-7 cm2/s (ouabain-treated). It was also determined that no gap junctional uncoupling occurs after extended exposure (up to 3.5 h) to a calcium-free extracellular environment.
Original language | English (US) |
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Pages (from-to) | 57-63 |
Number of pages | 7 |
Journal | Cell and Tissue Research |
Volume | 269 |
Issue number | 1 |
DOIs | |
State | Published - Jul 1992 |
Externally published | Yes |
Keywords
- Calcium-free solution
- Cornea
- Endothelium
- Gap junction
- Lucifer yellow
- Ouabain
- Phototoxicity
- Rabbit
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Histology
- Cell Biology