Dye coupling in the corneal endothelium: effects of ouabain and extracellular calcium removal

Mitchell A. Watsky, James L. Rae

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The effects of ouabain and extracellular calcium removal on gap junctional coupling of isolated rabbit corneal endothelium was examined using a modified dye-spread technique. This technique is a modification of a microelectrode procedure that now utilizes patch electrodes connected to a current-clamp circuit for dye iontophoresis and a shuttering system in the excitation light path to reduce phototoxic effects in the monolayer. It was found that a significant degree of junctional uncoupling occurred after 45 min of exposure to ouabain, quantified as a reduction in the effective diffusion coefficient of injected Lucifer yellow CH: 1.74×10-7 cm2/s (control) versus 0.43×10-7 cm2/s (ouabain-treated). It was also determined that no gap junctional uncoupling occurs after extended exposure (up to 3.5 h) to a calcium-free extracellular environment.

Original languageEnglish (US)
Pages (from-to)57-63
Number of pages7
JournalCell and Tissue Research
Volume269
Issue number1
DOIs
StatePublished - Jul 1 1992
Externally publishedYes

Keywords

  • Calcium-free solution
  • Cornea
  • Endothelium
  • Gap junction
  • Lucifer yellow
  • Ouabain
  • Phototoxicity
  • Rabbit

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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