Dynamics of nitric oxide, altered follicular microenvironment, and oocyte quality in women with endometriosis

Pravin T. Goud, Anuradha P. Goud, Narendra Joshi, Elizabeth Puscheck, Michael Peter Diamond, Husam M. Abu-Soud

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Objective To study follicular microenvironment in terms of free radical dynamics, oocyte quality, and assisted reproductive technology (ART) outcomes among women with (group A) and without (group B) endometriosis. Design Prospective cohort study. Setting University ART center. Patient(s) Women with and without endometriosis undergoing ART (n = 28). Intervention(s) Follicular fluid (FF), granulosa cells (GCs), immature oocytes (IOs), and ART data on sibling cohort oocytes in groups A and B were compared. Main Outcome Measure(s) ART live birth outcomes, maturation, and aging among in vitro matured (IVM) oocytes, nitrate levels in FF, and nitrotyrosine (NT) footprints and apoptosis in the GCs. Result(s) Clinical characteristics and ART live birth outcomes were no different between groups A and B. Women from group A had significantly lower peak serum E2 (2,068.8 ± 244.6 pg/mL vs. 2,756.2 ± 205.0 pg/mL) and higher apoptosis (80.0% vs. 22.2%) and NT staining (70.0% vs. 22.2%) in GCs compared with group B. Fewer IOs underwent IVM to MII (0.6 ± 0.3) in group A compared with group B (1.4 ± 0.2). IVM oocytes had significantly higher incidence of cortical granule loss (83.3% vs. 24.0%) and spindle disruption (66.7% vs. 16.0%) and higher zona pellucida dissolution timing (133.8 ± 9.4 s vs. 90.5 ± 5.8 s) in group A compared with group B. FF nitrate levels were significantly higher in women who failed to conceive in group A (478.2 ± 43.1 nmol/L) compared with those that did conceive (173.3 ± 19.0 nmol/L). Conclusion(s) Increased protein nitration, GC apoptosis, resistance to IVM, and oocyte aging indicate the involvement of oxidative dysregulation of NO in the pathophysiology of altered follicular milieu and poor oocyte quality in women with endometriosis.

Original languageEnglish (US)
JournalFertility and sterility
Volume102
Issue number1
DOIs
StatePublished - Jan 1 2014

Fingerprint

Endometriosis
Assisted Reproductive Techniques
Oocytes
Nitric Oxide
Granulosa Cells
Follicular Fluid
Live Birth
Apoptosis
Nitrates
Zona Pellucida
Free Radicals
Siblings
Cohort Studies
Outcome Assessment (Health Care)
Prospective Studies
Staining and Labeling
In Vitro Techniques
Incidence
Serum

Keywords

  • Endometriosis
  • nitric oxide
  • oocyte aging
  • oocyte quality
  • peroxynitrite
  • superoxide

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

Dynamics of nitric oxide, altered follicular microenvironment, and oocyte quality in women with endometriosis. / Goud, Pravin T.; Goud, Anuradha P.; Joshi, Narendra; Puscheck, Elizabeth; Diamond, Michael Peter; Abu-Soud, Husam M.

In: Fertility and sterility, Vol. 102, No. 1, 01.01.2014.

Research output: Contribution to journalArticle

Goud, Pravin T. ; Goud, Anuradha P. ; Joshi, Narendra ; Puscheck, Elizabeth ; Diamond, Michael Peter ; Abu-Soud, Husam M. / Dynamics of nitric oxide, altered follicular microenvironment, and oocyte quality in women with endometriosis. In: Fertility and sterility. 2014 ; Vol. 102, No. 1.
@article{fefdb222f3724a9490321b4c30912eeb,
title = "Dynamics of nitric oxide, altered follicular microenvironment, and oocyte quality in women with endometriosis",
abstract = "Objective To study follicular microenvironment in terms of free radical dynamics, oocyte quality, and assisted reproductive technology (ART) outcomes among women with (group A) and without (group B) endometriosis. Design Prospective cohort study. Setting University ART center. Patient(s) Women with and without endometriosis undergoing ART (n = 28). Intervention(s) Follicular fluid (FF), granulosa cells (GCs), immature oocytes (IOs), and ART data on sibling cohort oocytes in groups A and B were compared. Main Outcome Measure(s) ART live birth outcomes, maturation, and aging among in vitro matured (IVM) oocytes, nitrate levels in FF, and nitrotyrosine (NT) footprints and apoptosis in the GCs. Result(s) Clinical characteristics and ART live birth outcomes were no different between groups A and B. Women from group A had significantly lower peak serum E2 (2,068.8 ± 244.6 pg/mL vs. 2,756.2 ± 205.0 pg/mL) and higher apoptosis (80.0{\%} vs. 22.2{\%}) and NT staining (70.0{\%} vs. 22.2{\%}) in GCs compared with group B. Fewer IOs underwent IVM to MII (0.6 ± 0.3) in group A compared with group B (1.4 ± 0.2). IVM oocytes had significantly higher incidence of cortical granule loss (83.3{\%} vs. 24.0{\%}) and spindle disruption (66.7{\%} vs. 16.0{\%}) and higher zona pellucida dissolution timing (133.8 ± 9.4 s vs. 90.5 ± 5.8 s) in group A compared with group B. FF nitrate levels were significantly higher in women who failed to conceive in group A (478.2 ± 43.1 nmol/L) compared with those that did conceive (173.3 ± 19.0 nmol/L). Conclusion(s) Increased protein nitration, GC apoptosis, resistance to IVM, and oocyte aging indicate the involvement of oxidative dysregulation of NO in the pathophysiology of altered follicular milieu and poor oocyte quality in women with endometriosis.",
keywords = "Endometriosis, nitric oxide, oocyte aging, oocyte quality, peroxynitrite, superoxide",
author = "Goud, {Pravin T.} and Goud, {Anuradha P.} and Narendra Joshi and Elizabeth Puscheck and Diamond, {Michael Peter} and Abu-Soud, {Husam M.}",
year = "2014",
month = "1",
day = "1",
doi = "10.1016/j.fertnstert.2014.03.053",
language = "English (US)",
volume = "102",
journal = "Fertility and Sterility",
issn = "0015-0282",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - Dynamics of nitric oxide, altered follicular microenvironment, and oocyte quality in women with endometriosis

AU - Goud, Pravin T.

AU - Goud, Anuradha P.

AU - Joshi, Narendra

AU - Puscheck, Elizabeth

AU - Diamond, Michael Peter

AU - Abu-Soud, Husam M.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Objective To study follicular microenvironment in terms of free radical dynamics, oocyte quality, and assisted reproductive technology (ART) outcomes among women with (group A) and without (group B) endometriosis. Design Prospective cohort study. Setting University ART center. Patient(s) Women with and without endometriosis undergoing ART (n = 28). Intervention(s) Follicular fluid (FF), granulosa cells (GCs), immature oocytes (IOs), and ART data on sibling cohort oocytes in groups A and B were compared. Main Outcome Measure(s) ART live birth outcomes, maturation, and aging among in vitro matured (IVM) oocytes, nitrate levels in FF, and nitrotyrosine (NT) footprints and apoptosis in the GCs. Result(s) Clinical characteristics and ART live birth outcomes were no different between groups A and B. Women from group A had significantly lower peak serum E2 (2,068.8 ± 244.6 pg/mL vs. 2,756.2 ± 205.0 pg/mL) and higher apoptosis (80.0% vs. 22.2%) and NT staining (70.0% vs. 22.2%) in GCs compared with group B. Fewer IOs underwent IVM to MII (0.6 ± 0.3) in group A compared with group B (1.4 ± 0.2). IVM oocytes had significantly higher incidence of cortical granule loss (83.3% vs. 24.0%) and spindle disruption (66.7% vs. 16.0%) and higher zona pellucida dissolution timing (133.8 ± 9.4 s vs. 90.5 ± 5.8 s) in group A compared with group B. FF nitrate levels were significantly higher in women who failed to conceive in group A (478.2 ± 43.1 nmol/L) compared with those that did conceive (173.3 ± 19.0 nmol/L). Conclusion(s) Increased protein nitration, GC apoptosis, resistance to IVM, and oocyte aging indicate the involvement of oxidative dysregulation of NO in the pathophysiology of altered follicular milieu and poor oocyte quality in women with endometriosis.

AB - Objective To study follicular microenvironment in terms of free radical dynamics, oocyte quality, and assisted reproductive technology (ART) outcomes among women with (group A) and without (group B) endometriosis. Design Prospective cohort study. Setting University ART center. Patient(s) Women with and without endometriosis undergoing ART (n = 28). Intervention(s) Follicular fluid (FF), granulosa cells (GCs), immature oocytes (IOs), and ART data on sibling cohort oocytes in groups A and B were compared. Main Outcome Measure(s) ART live birth outcomes, maturation, and aging among in vitro matured (IVM) oocytes, nitrate levels in FF, and nitrotyrosine (NT) footprints and apoptosis in the GCs. Result(s) Clinical characteristics and ART live birth outcomes were no different between groups A and B. Women from group A had significantly lower peak serum E2 (2,068.8 ± 244.6 pg/mL vs. 2,756.2 ± 205.0 pg/mL) and higher apoptosis (80.0% vs. 22.2%) and NT staining (70.0% vs. 22.2%) in GCs compared with group B. Fewer IOs underwent IVM to MII (0.6 ± 0.3) in group A compared with group B (1.4 ± 0.2). IVM oocytes had significantly higher incidence of cortical granule loss (83.3% vs. 24.0%) and spindle disruption (66.7% vs. 16.0%) and higher zona pellucida dissolution timing (133.8 ± 9.4 s vs. 90.5 ± 5.8 s) in group A compared with group B. FF nitrate levels were significantly higher in women who failed to conceive in group A (478.2 ± 43.1 nmol/L) compared with those that did conceive (173.3 ± 19.0 nmol/L). Conclusion(s) Increased protein nitration, GC apoptosis, resistance to IVM, and oocyte aging indicate the involvement of oxidative dysregulation of NO in the pathophysiology of altered follicular milieu and poor oocyte quality in women with endometriosis.

KW - Endometriosis

KW - nitric oxide

KW - oocyte aging

KW - oocyte quality

KW - peroxynitrite

KW - superoxide

UR - http://www.scopus.com/inward/record.url?scp=84903387507&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84903387507&partnerID=8YFLogxK

U2 - 10.1016/j.fertnstert.2014.03.053

DO - 10.1016/j.fertnstert.2014.03.053

M3 - Article

C2 - 24825428

AN - SCOPUS:84903387507

VL - 102

JO - Fertility and Sterility

JF - Fertility and Sterility

SN - 0015-0282

IS - 1

ER -