Dynamin GTPase regulated by protein kinase C phosphorylation in nerve terminals

Phillip J. Robinson, Jean Marie Sontag, Jun Ping Liu, Else Marie Fykse, Clive Slaughter, Harvey McMahontt, Thomas C. Südhof

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Abstract

DYNAMIN is a microtubule-binding protein with a microtubule-activated GTPase activity1,3. The gene encoding dynamin is mut-ated in shibire4,5, a Drosophila mutant defective in endocytosis in nerve terminals and other cells6-9. These observations place dyna-min into two distinct functional contexts, suggesting roles in microtubule-based motility or in endocytosis. We report here that dynamin is identical to the neuronal phosphoprotein dephosphin (P96), originally identified by its stimulus-dependent dephosphorylation in nerve terminals10-13. Dynamin is a protein doublet of Mr 94 and 96K arising by alternative splicing of its primary transcript. In the nerve terminal, both forms of dynamin are phosphorylated by protein kinase C (PKC) and are quantitatively dephosphoryla-ted on excitation. In vitro, dynamin is also phosphorylated by casein kinase II which inhibits PKC phosphorylation. Phosphory-lation by PKC but not by casein kinase II enhances the GTPase activity of dynamin 12-fold. The dynamins are therefore a group of nerve terminal phosphoproteins whose GTPase is regulated by phosphorylation in parallel with synaptic vesicle recycling. The regulation of dynamin GTPase could serve as the trigger for the rapid endocytosis of synaptic vesicles after exocytosis.

Original languageEnglish (US)
Pages (from-to)163-166
Number of pages4
JournalNature
Volume365
Issue number6442
DOIs
StatePublished - Jan 1 1993

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Dynamins
GTP Phosphohydrolases
Protein Kinase C
Phosphorylation
Endocytosis
Casein Kinase II
Synaptic Vesicles
Phosphoproteins
Microtubules
Microtubule Proteins
Exocytosis
Alternative Splicing
Drosophila
Carrier Proteins

ASJC Scopus subject areas

  • General

Cite this

Robinson, P. J., Sontag, J. M., Liu, J. P., Fykse, E. M., Slaughter, C., McMahontt, H., & Südhof, T. C. (1993). Dynamin GTPase regulated by protein kinase C phosphorylation in nerve terminals. Nature, 365(6442), 163-166. https://doi.org/10.1038/365163a0

Dynamin GTPase regulated by protein kinase C phosphorylation in nerve terminals. / Robinson, Phillip J.; Sontag, Jean Marie; Liu, Jun Ping; Fykse, Else Marie; Slaughter, Clive; McMahontt, Harvey; Südhof, Thomas C.

In: Nature, Vol. 365, No. 6442, 01.01.1993, p. 163-166.

Research output: Contribution to journalArticle

Robinson, PJ, Sontag, JM, Liu, JP, Fykse, EM, Slaughter, C, McMahontt, H & Südhof, TC 1993, 'Dynamin GTPase regulated by protein kinase C phosphorylation in nerve terminals', Nature, vol. 365, no. 6442, pp. 163-166. https://doi.org/10.1038/365163a0
Robinson PJ, Sontag JM, Liu JP, Fykse EM, Slaughter C, McMahontt H et al. Dynamin GTPase regulated by protein kinase C phosphorylation in nerve terminals. Nature. 1993 Jan 1;365(6442):163-166. https://doi.org/10.1038/365163a0
Robinson, Phillip J. ; Sontag, Jean Marie ; Liu, Jun Ping ; Fykse, Else Marie ; Slaughter, Clive ; McMahontt, Harvey ; Südhof, Thomas C. / Dynamin GTPase regulated by protein kinase C phosphorylation in nerve terminals. In: Nature. 1993 ; Vol. 365, No. 6442. pp. 163-166.
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