Dynamin-like protein 1 is involved in peroxisomal fission

Annett Koch, Meinolf Thiemann, Markus Grabenbauer, Yisang Yoon, Mark A. McNiven, Michael Schrader

Research output: Contribution to journalArticle

233 Citations (Scopus)

Abstract

The mammalian dynamin-like protein 1 (DLP1), a member of the dynamin family of large GTPases, possesses mechanochemical properties known to constrict and tubulate membranes. In this study, we have combined two experimental approaches, induction of peroxisome proliferation by Pex11pβ and expression of dominant-negative mutants, to test whether DLP1 plays a role in peroxisomal growth and division. We were able to localize DLP1 in spots on tubular peroxisomes in HepG2 cells. In addition, immunoblot analysis revealed the presence of DLP1 in highly purified peroxisomal fractions from rat liver and an increase of DLP1 after treatment of rats with the peroxisome proliferator bezafibrate. Expression of a dominant negative DLP1 mutant deficient in GTP hydrolysis (K38A) either alone or in combination with Pex11pβ caused the appearance of tubular peroxisomes but had no influence on their intracellular distribution. In co-expressing cells, the formation of tubulo-reticular networks of peroxisomes was promoted, and peroxisomal division was completely inhibited. These findings were confirmed by silencing of DLP1 using siRNA. We propose a direct role for the dynamin-like protein DLP1 in peroxisomal fission and in the maintenance of peroxisomal morphology in mammalian cells.

Original languageEnglish (US)
Pages (from-to)8597-8605
Number of pages9
JournalJournal of Biological Chemistry
Volume278
Issue number10
DOIs
StatePublished - Mar 7 2003
Externally publishedYes

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Dynamins
Peroxisomes
Proteins
Rats
Bezafibrate
Peroxisome Proliferators
Reticular Formation
GTP Phosphohydrolases
Hep G2 Cells
Mutant Proteins
Guanosine Triphosphate
Liver
Small Interfering RNA
Hydrolysis
Cells
Maintenance

ASJC Scopus subject areas

  • Biochemistry

Cite this

Koch, A., Thiemann, M., Grabenbauer, M., Yoon, Y., McNiven, M. A., & Schrader, M. (2003). Dynamin-like protein 1 is involved in peroxisomal fission. Journal of Biological Chemistry, 278(10), 8597-8605. https://doi.org/10.1074/jbc.M211761200

Dynamin-like protein 1 is involved in peroxisomal fission. / Koch, Annett; Thiemann, Meinolf; Grabenbauer, Markus; Yoon, Yisang; McNiven, Mark A.; Schrader, Michael.

In: Journal of Biological Chemistry, Vol. 278, No. 10, 07.03.2003, p. 8597-8605.

Research output: Contribution to journalArticle

Koch, A, Thiemann, M, Grabenbauer, M, Yoon, Y, McNiven, MA & Schrader, M 2003, 'Dynamin-like protein 1 is involved in peroxisomal fission', Journal of Biological Chemistry, vol. 278, no. 10, pp. 8597-8605. https://doi.org/10.1074/jbc.M211761200
Koch A, Thiemann M, Grabenbauer M, Yoon Y, McNiven MA, Schrader M. Dynamin-like protein 1 is involved in peroxisomal fission. Journal of Biological Chemistry. 2003 Mar 7;278(10):8597-8605. https://doi.org/10.1074/jbc.M211761200
Koch, Annett ; Thiemann, Meinolf ; Grabenbauer, Markus ; Yoon, Yisang ; McNiven, Mark A. ; Schrader, Michael. / Dynamin-like protein 1 is involved in peroxisomal fission. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 10. pp. 8597-8605.
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