Dynein associates with oskar mRNPs and is required for their efficient net plus-end localization in Drosophila oocytes

Paulomi Sanghavi, Shobha Laxani, Xuan Li, Simon L. Bullock, Graydon Bennett Gonsalvez

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

In order for eukaryotic cells to function properly, they must establish polarity. The Drosophila oocyte uses mRNA localization to establish polarity and hence provides a genetically tractable model in which to study this process. The spatial restriction of oskar mRNA and its subsequent protein product is necessary for embryonic patterning. The localization of oskar mRNA requires microtubules and microtubule-based motor proteins. Null mutants in Kinesin heavy chain (Khc), the motor subunit of the plus end-directed Kinesin-1, result in oskar mRNA delocalization. Although the majority of oskar particles are non-motile in khc nulls, a small fraction of particles display active motility. Thus, a motor other than Kinesin-1 could conceivably also participate in oskar mRNA localization. Here we show that Dynein heavy chain (Dhc), the motor subunit of the minus end-directed Dynein complex, extensively co-localizes with Khc and oskar mRNA. In addition, immunoprecipitation of the Dynein complex specifically co-precipitated oskar mRNA and Khc. Lastly, germline-specific depletion of Dhc resulted in oskar mRNA and Khc delocalization. Our results therefore suggest that efficient posterior localization of oskar mRNA requires the concerted activities of both Dynein and Kinesin-1.

Original languageEnglish (US)
Article numbere80605
JournalPloS one
Volume8
Issue number11
DOIs
StatePublished - Nov 11 2013

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Dyneins
kinesin
Kinesin
Drosophila
Oocytes
oocytes
Messenger RNA
microtubules
Microtubules
protein products
messenger ribonucleoprotein
eukaryotic cells
germ cells
Eukaryotic Cells
Immunoprecipitation
Proteins
mutants

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Dynein associates with oskar mRNPs and is required for their efficient net plus-end localization in Drosophila oocytes. / Sanghavi, Paulomi; Laxani, Shobha; Li, Xuan; Bullock, Simon L.; Gonsalvez, Graydon Bennett.

In: PloS one, Vol. 8, No. 11, e80605, 11.11.2013.

Research output: Contribution to journalArticle

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