Dysplastic hepatocytes develop nuclear inclusions in a mouse model of viral hepatitis

Priyanka Thakur, Folami Lamoke Powell, Joanna M. Chaffin, Manuela Bartoli, Jeffrey R Lee, Michael B Duncan

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Viral hepatitis resulting in chronic liver disease is an important clinical challenge and insight into the cellular processes that drive pathogenesis will be critical in order to develop new diagnostic and therapeutic options. Nuclear inclusions in viral and non-viral hepatitis are well documented and have diagnostic significance in some disease contexts. However, the origins and functional consequences of these nuclear inclusions remain elusive. To date the clinical observation of nuclear inclusions in viral and non-viral hepatitis has not been explored at depth in murine models of liver disease. Herein, we report that in a transgenic model of hepatitis B surface antigen mediated hepatitis, murine hepatocytes exhibit nuclear inclusions. Cells bearing nuclear inclusions were more likely to express markers of cell proliferation. We also established a correlation between these inclusions and oxidative stress. N-acetyl cysteine treatment effectively reduced oxidative stress levels, relieved endoplasmic reticulum (ER) stress, and the number of nuclear inclusions we observed in the transgenic mice. Our results suggest that the presence of nuclear inclusions in hepatocytes correlates with oxidative stress and cellular proliferation in a model of antigen mediated hepatitis.

Original languageEnglish (US)
Article numbere99872
JournalPloS one
Volume9
Issue number6
DOIs
StatePublished - Jun 16 2014

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nuclear inclusions
viral hepatitis
Intranuclear Inclusion Bodies
Hepatitis
hepatocytes
Hepatocytes
animal models
hepatitis
Oxidative stress
Oxidative Stress
oxidative stress
liver diseases
Liver Diseases
cell proliferation
Liver
Hepatitis Antigens
Cell Proliferation
genetically modified organisms
Bearings (structural)
Acetylcysteine

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Dysplastic hepatocytes develop nuclear inclusions in a mouse model of viral hepatitis. / Thakur, Priyanka; Powell, Folami Lamoke; Chaffin, Joanna M.; Bartoli, Manuela; Lee, Jeffrey R; Duncan, Michael B.

In: PloS one, Vol. 9, No. 6, e99872, 16.06.2014.

Research output: Contribution to journalArticle

Thakur, Priyanka ; Powell, Folami Lamoke ; Chaffin, Joanna M. ; Bartoli, Manuela ; Lee, Jeffrey R ; Duncan, Michael B. / Dysplastic hepatocytes develop nuclear inclusions in a mouse model of viral hepatitis. In: PloS one. 2014 ; Vol. 9, No. 6.
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