Effect of 2% chlorhexidine on dentin microtensile bond strengths and nanoleakage of luting cements

N. Hiraishi, C. K.Y. Yiu, N. M. King, Franklin Chi Meng Tay

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Objectives: The aim of this study was to investigate the effect of pre-treatment by chlorhexidine on the microtensile bond strength (mTBS) of resin cements and nanoleakage at the resin-dentine interfaces. Methods: Cylindrical composite blocks were luted to human dentine using resin cements (RelyX ARC, 3M ESPE: ARC; Panavia F, Kuraray Medical Inc.: PF; RelyX Unicem, 3M ESPE: UN) with/without pre-treatment by 2% chlorhexidine digluconate (CAVITY CLEANSER, Bisco, Inc., Schaumburg, IL, USA). CAVITY CLEANSER was applied on the acid etched dentine for 60 s in the ARC group, and on smear layer-covered dentine in the PF and UN groups. After storage in water for 24 h, the bonded teeth were sectioned into 1 mm thick slabs and further into 0.9 mm × 0.9 mm beams. After immersion in water or ammoniacal silver nitrate for 24 h, the beams were stressed to failure in tension. The fractured surfaces were examined by field-emission scanning electron microscopy (FE-SEM) using backscattered electron mode. The silver-stained slabs were used to examine nanoleakage within the bonded interface by FE-SEM. Results: The resin cement and chlorhexidine treatment had significant effects (p < 0.0001) on mTBS; while the storage media had no significant effect (p = 0.435). The mTBS of ARC was significantly higher than the other cements. Chlorhexidine reduced mTBS and produced pronounced nanoleakage when PF and UC were luted to dentine. Conclusions: Pre-treatment with chlorhexidine affected the integrity of dentine bonding with PF and UC, while there was no adverse effect on coupling of ARC.

Original languageEnglish (US)
Pages (from-to)440-448
Number of pages9
JournalJournal of Dentistry
Volume37
Issue number6
DOIs
StatePublished - Jun 1 2009

Fingerprint

Chlorhexidine
Dentin
AIDS-Related Complex
Resin Cements
United Nations
Electron Scanning Microscopy
Smear Layer
Silver Nitrate
Water
Immersion
Silver
Tooth
Electrons
Acids

Keywords

  • Chlorhexidine
  • Dentine disinfectant
  • Microtensile bond strength
  • Nanoleakage
  • Resin cement
  • SEM

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Effect of 2% chlorhexidine on dentin microtensile bond strengths and nanoleakage of luting cements. / Hiraishi, N.; Yiu, C. K.Y.; King, N. M.; Tay, Franklin Chi Meng.

In: Journal of Dentistry, Vol. 37, No. 6, 01.06.2009, p. 440-448.

Research output: Contribution to journalArticle

@article{da5fce633e8a4696994554ddf7917eab,
title = "Effect of 2{\%} chlorhexidine on dentin microtensile bond strengths and nanoleakage of luting cements",
abstract = "Objectives: The aim of this study was to investigate the effect of pre-treatment by chlorhexidine on the microtensile bond strength (mTBS) of resin cements and nanoleakage at the resin-dentine interfaces. Methods: Cylindrical composite blocks were luted to human dentine using resin cements (RelyX ARC, 3M ESPE: ARC; Panavia F, Kuraray Medical Inc.: PF; RelyX Unicem, 3M ESPE: UN) with/without pre-treatment by 2{\%} chlorhexidine digluconate (CAVITY CLEANSER, Bisco, Inc., Schaumburg, IL, USA). CAVITY CLEANSER was applied on the acid etched dentine for 60 s in the ARC group, and on smear layer-covered dentine in the PF and UN groups. After storage in water for 24 h, the bonded teeth were sectioned into 1 mm thick slabs and further into 0.9 mm × 0.9 mm beams. After immersion in water or ammoniacal silver nitrate for 24 h, the beams were stressed to failure in tension. The fractured surfaces were examined by field-emission scanning electron microscopy (FE-SEM) using backscattered electron mode. The silver-stained slabs were used to examine nanoleakage within the bonded interface by FE-SEM. Results: The resin cement and chlorhexidine treatment had significant effects (p < 0.0001) on mTBS; while the storage media had no significant effect (p = 0.435). The mTBS of ARC was significantly higher than the other cements. Chlorhexidine reduced mTBS and produced pronounced nanoleakage when PF and UC were luted to dentine. Conclusions: Pre-treatment with chlorhexidine affected the integrity of dentine bonding with PF and UC, while there was no adverse effect on coupling of ARC.",
keywords = "Chlorhexidine, Dentine disinfectant, Microtensile bond strength, Nanoleakage, Resin cement, SEM",
author = "N. Hiraishi and Yiu, {C. K.Y.} and King, {N. M.} and Tay, {Franklin Chi Meng}",
year = "2009",
month = "6",
day = "1",
doi = "10.1016/j.jdent.2009.02.002",
language = "English (US)",
volume = "37",
pages = "440--448",
journal = "Journal of Dentistry",
issn = "0300-5712",
publisher = "Elsevier BV",
number = "6",

}

TY - JOUR

T1 - Effect of 2% chlorhexidine on dentin microtensile bond strengths and nanoleakage of luting cements

AU - Hiraishi, N.

AU - Yiu, C. K.Y.

AU - King, N. M.

AU - Tay, Franklin Chi Meng

PY - 2009/6/1

Y1 - 2009/6/1

N2 - Objectives: The aim of this study was to investigate the effect of pre-treatment by chlorhexidine on the microtensile bond strength (mTBS) of resin cements and nanoleakage at the resin-dentine interfaces. Methods: Cylindrical composite blocks were luted to human dentine using resin cements (RelyX ARC, 3M ESPE: ARC; Panavia F, Kuraray Medical Inc.: PF; RelyX Unicem, 3M ESPE: UN) with/without pre-treatment by 2% chlorhexidine digluconate (CAVITY CLEANSER, Bisco, Inc., Schaumburg, IL, USA). CAVITY CLEANSER was applied on the acid etched dentine for 60 s in the ARC group, and on smear layer-covered dentine in the PF and UN groups. After storage in water for 24 h, the bonded teeth were sectioned into 1 mm thick slabs and further into 0.9 mm × 0.9 mm beams. After immersion in water or ammoniacal silver nitrate for 24 h, the beams were stressed to failure in tension. The fractured surfaces were examined by field-emission scanning electron microscopy (FE-SEM) using backscattered electron mode. The silver-stained slabs were used to examine nanoleakage within the bonded interface by FE-SEM. Results: The resin cement and chlorhexidine treatment had significant effects (p < 0.0001) on mTBS; while the storage media had no significant effect (p = 0.435). The mTBS of ARC was significantly higher than the other cements. Chlorhexidine reduced mTBS and produced pronounced nanoleakage when PF and UC were luted to dentine. Conclusions: Pre-treatment with chlorhexidine affected the integrity of dentine bonding with PF and UC, while there was no adverse effect on coupling of ARC.

AB - Objectives: The aim of this study was to investigate the effect of pre-treatment by chlorhexidine on the microtensile bond strength (mTBS) of resin cements and nanoleakage at the resin-dentine interfaces. Methods: Cylindrical composite blocks were luted to human dentine using resin cements (RelyX ARC, 3M ESPE: ARC; Panavia F, Kuraray Medical Inc.: PF; RelyX Unicem, 3M ESPE: UN) with/without pre-treatment by 2% chlorhexidine digluconate (CAVITY CLEANSER, Bisco, Inc., Schaumburg, IL, USA). CAVITY CLEANSER was applied on the acid etched dentine for 60 s in the ARC group, and on smear layer-covered dentine in the PF and UN groups. After storage in water for 24 h, the bonded teeth were sectioned into 1 mm thick slabs and further into 0.9 mm × 0.9 mm beams. After immersion in water or ammoniacal silver nitrate for 24 h, the beams were stressed to failure in tension. The fractured surfaces were examined by field-emission scanning electron microscopy (FE-SEM) using backscattered electron mode. The silver-stained slabs were used to examine nanoleakage within the bonded interface by FE-SEM. Results: The resin cement and chlorhexidine treatment had significant effects (p < 0.0001) on mTBS; while the storage media had no significant effect (p = 0.435). The mTBS of ARC was significantly higher than the other cements. Chlorhexidine reduced mTBS and produced pronounced nanoleakage when PF and UC were luted to dentine. Conclusions: Pre-treatment with chlorhexidine affected the integrity of dentine bonding with PF and UC, while there was no adverse effect on coupling of ARC.

KW - Chlorhexidine

KW - Dentine disinfectant

KW - Microtensile bond strength

KW - Nanoleakage

KW - Resin cement

KW - SEM

UR - http://www.scopus.com/inward/record.url?scp=64549129100&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=64549129100&partnerID=8YFLogxK

U2 - 10.1016/j.jdent.2009.02.002

DO - 10.1016/j.jdent.2009.02.002

M3 - Article

VL - 37

SP - 440

EP - 448

JO - Journal of Dentistry

JF - Journal of Dentistry

SN - 0300-5712

IS - 6

ER -