Effect of a novel quaternary ammonium silane cavity disinfectant on durability of resin–dentine bond

D. Daood, C. K.Y. Yiu, M. F. Burrow, L. N. Niu, Franklin Chi Meng Tay

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objective The present study examined the effect of a quaternary ammonium silane (QAS) cavity disinfectant on the viability of human dental pulp cells, dentine bond durability and nanoleakage of simplified etch-and-rinse adhesives. Methods Etched dentine surface of third molars were randomly divided into two adhesive groups, Adper™ Single Bond 2 and Prime & Bond® NT™. For each adhesive, the teeth were randomly assigned to five cavity disinfectant groups (N = 6): Group 1: deionised water (control); Group 2: 2% chlorhexidine (CHX); Group 3: 2% QAS; Group 4: 5% QAS and Group 5: 10% QAS. The cavity disinfectants were applied on etched dentine surfaces for 20 s, followed by adhesive application. The bonded teeth were sectioned for bond strength testing at 24 h, 6 months and 12 months. Viability of human dental pulpal cells was examined using MTT assay. Bond strength data were analysed using 3-way ANOVA and Tukey test. Interfacial nanoleakage was evaluated after 24 h and 12 months and analysed using Kruskal–Wallis test. Results Significant differences in bond strength were observed for the factors disinfectants (p < 0.001) and time (p < 0.001); while the factor, adhesive, was not significantly different (p = 0.203). The 2% QAS cavity disinfectant preserved bond strength of both adhesives and reduced interfacial nanoleakage after 12 months. Cell viability was the lowest for 2% CHX, followed by 2% QAS and the control. Conclusions The 2% QAS cavity disinfectant demonstrated greater cell viability compared to 2% CHX, with no adverse effect on immediate bond strength and preserved bond stability over time. Clinical significance Incorporation of 2% quaternary ammonium silane cavity disinfectant in the resin–dentine bonding protocol enhances the success rate of bonded restorations.

Original languageEnglish (US)
Pages (from-to)77-86
Number of pages10
JournalJournal of Dentistry
Volume60
DOIs
StatePublished - May 1 2017

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Silanes
Disinfectants
Ammonium Compounds
Adhesives
Chlorhexidine
Dentin
Tooth
Cell Survival
Dental Pulp
Third Molar
Analysis of Variance
Water

Keywords

  • Cysteine cathepsins
  • Dentine
  • Matrix metalloproteinases
  • Quaternary ammonium silane

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Effect of a novel quaternary ammonium silane cavity disinfectant on durability of resin–dentine bond. / Daood, D.; Yiu, C. K.Y.; Burrow, M. F.; Niu, L. N.; Tay, Franklin Chi Meng.

In: Journal of Dentistry, Vol. 60, 01.05.2017, p. 77-86.

Research output: Contribution to journalArticle

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abstract = "Objective The present study examined the effect of a quaternary ammonium silane (QAS) cavity disinfectant on the viability of human dental pulp cells, dentine bond durability and nanoleakage of simplified etch-and-rinse adhesives. Methods Etched dentine surface of third molars were randomly divided into two adhesive groups, Adper™ Single Bond 2 and Prime & Bond{\circledR} NT™. For each adhesive, the teeth were randomly assigned to five cavity disinfectant groups (N = 6): Group 1: deionised water (control); Group 2: 2{\%} chlorhexidine (CHX); Group 3: 2{\%} QAS; Group 4: 5{\%} QAS and Group 5: 10{\%} QAS. The cavity disinfectants were applied on etched dentine surfaces for 20 s, followed by adhesive application. The bonded teeth were sectioned for bond strength testing at 24 h, 6 months and 12 months. Viability of human dental pulpal cells was examined using MTT assay. Bond strength data were analysed using 3-way ANOVA and Tukey test. Interfacial nanoleakage was evaluated after 24 h and 12 months and analysed using Kruskal–Wallis test. Results Significant differences in bond strength were observed for the factors disinfectants (p < 0.001) and time (p < 0.001); while the factor, adhesive, was not significantly different (p = 0.203). The 2{\%} QAS cavity disinfectant preserved bond strength of both adhesives and reduced interfacial nanoleakage after 12 months. Cell viability was the lowest for 2{\%} CHX, followed by 2{\%} QAS and the control. Conclusions The 2{\%} QAS cavity disinfectant demonstrated greater cell viability compared to 2{\%} CHX, with no adverse effect on immediate bond strength and preserved bond stability over time. Clinical significance Incorporation of 2{\%} quaternary ammonium silane cavity disinfectant in the resin–dentine bonding protocol enhances the success rate of bonded restorations.",
keywords = "Cysteine cathepsins, Dentine, Matrix metalloproteinases, Quaternary ammonium silane",
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AU - Yiu, C. K.Y.

AU - Burrow, M. F.

AU - Niu, L. N.

AU - Tay, Franklin Chi Meng

PY - 2017/5/1

Y1 - 2017/5/1

N2 - Objective The present study examined the effect of a quaternary ammonium silane (QAS) cavity disinfectant on the viability of human dental pulp cells, dentine bond durability and nanoleakage of simplified etch-and-rinse adhesives. Methods Etched dentine surface of third molars were randomly divided into two adhesive groups, Adper™ Single Bond 2 and Prime & Bond® NT™. For each adhesive, the teeth were randomly assigned to five cavity disinfectant groups (N = 6): Group 1: deionised water (control); Group 2: 2% chlorhexidine (CHX); Group 3: 2% QAS; Group 4: 5% QAS and Group 5: 10% QAS. The cavity disinfectants were applied on etched dentine surfaces for 20 s, followed by adhesive application. The bonded teeth were sectioned for bond strength testing at 24 h, 6 months and 12 months. Viability of human dental pulpal cells was examined using MTT assay. Bond strength data were analysed using 3-way ANOVA and Tukey test. Interfacial nanoleakage was evaluated after 24 h and 12 months and analysed using Kruskal–Wallis test. Results Significant differences in bond strength were observed for the factors disinfectants (p < 0.001) and time (p < 0.001); while the factor, adhesive, was not significantly different (p = 0.203). The 2% QAS cavity disinfectant preserved bond strength of both adhesives and reduced interfacial nanoleakage after 12 months. Cell viability was the lowest for 2% CHX, followed by 2% QAS and the control. Conclusions The 2% QAS cavity disinfectant demonstrated greater cell viability compared to 2% CHX, with no adverse effect on immediate bond strength and preserved bond stability over time. Clinical significance Incorporation of 2% quaternary ammonium silane cavity disinfectant in the resin–dentine bonding protocol enhances the success rate of bonded restorations.

AB - Objective The present study examined the effect of a quaternary ammonium silane (QAS) cavity disinfectant on the viability of human dental pulp cells, dentine bond durability and nanoleakage of simplified etch-and-rinse adhesives. Methods Etched dentine surface of third molars were randomly divided into two adhesive groups, Adper™ Single Bond 2 and Prime & Bond® NT™. For each adhesive, the teeth were randomly assigned to five cavity disinfectant groups (N = 6): Group 1: deionised water (control); Group 2: 2% chlorhexidine (CHX); Group 3: 2% QAS; Group 4: 5% QAS and Group 5: 10% QAS. The cavity disinfectants were applied on etched dentine surfaces for 20 s, followed by adhesive application. The bonded teeth were sectioned for bond strength testing at 24 h, 6 months and 12 months. Viability of human dental pulpal cells was examined using MTT assay. Bond strength data were analysed using 3-way ANOVA and Tukey test. Interfacial nanoleakage was evaluated after 24 h and 12 months and analysed using Kruskal–Wallis test. Results Significant differences in bond strength were observed for the factors disinfectants (p < 0.001) and time (p < 0.001); while the factor, adhesive, was not significantly different (p = 0.203). The 2% QAS cavity disinfectant preserved bond strength of both adhesives and reduced interfacial nanoleakage after 12 months. Cell viability was the lowest for 2% CHX, followed by 2% QAS and the control. Conclusions The 2% QAS cavity disinfectant demonstrated greater cell viability compared to 2% CHX, with no adverse effect on immediate bond strength and preserved bond stability over time. Clinical significance Incorporation of 2% quaternary ammonium silane cavity disinfectant in the resin–dentine bonding protocol enhances the success rate of bonded restorations.

KW - Cysteine cathepsins

KW - Dentine

KW - Matrix metalloproteinases

KW - Quaternary ammonium silane

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