Effect of a novel quaternary ammonium silane on dentin protease activities

D. Umer, C. K.Y. Yiu, M. F. Burrow, L. N. Niu, Franklin Chi Meng Tay

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Objectives Demineralized dentin collagen release C-terminal cross-linked telopeptide (ICTP) and C-terminal peptide (CTX) during degradation. The present study evaluated the effects of dentin pre-treatment with K21, a quaternary ammonium silane (QAS), on matrix metalloproteinase (MMP) and cathepsin K-mediated collagen degradation. Methods Dentin beams were demineralized with 10% H3PO4 for 24 h. After baseline dry mass measurements, the beams were divided into 5 groups (N = 10) according to protease inhibitors. The beams were pre-treated for 2 min with 2% chlorhexidine (CHX), 2%, 5% or 10% QAS; no pre-treatment was performed for the control group. The beams were subsequently incubated in calcium- and zinc-containing medium for 3, 7 or 14 days, after which changes in dry mass were measured and incubation media were examined for ICTP and CTX release. The MMP-2 and cathepsin K activities in QAS-treated dentin powder were also quantified using ELISA. Results The two factors (disinfectants and time) had a significant effect on dry mass loss, ICTP and CTX release (p < 0.001). The percentage of dry mass loss increased with time and was significantly lower in all experimental groups when compared to the control at 14 days (p < 0.001). Conversely, the rate of ICTP and CTX release was significantly lower in the experimental groups, compared to the uninhibited control at 7 and 14 days (p < 0.001). Dentinal MMP-2 and cathepsin K activities were significantly reduced after demineralized dentin was pre-treated with QAS. Conclusion The experimental QAS is a good inhibitor of MMP and cathepsin K activities in demineralized dentin. Clinical significance The newly developed antibacterial quaternary ammonium silane increases the resistance of dentin collagen to degradation by inhibiting endogenous matrix metalloproteinases and cysteine cathepsins. The quaternary ammonium silane cavity disinfectant is promising for use as a protease inhibitor to improve durability of resin-dentin bonds.

Original languageEnglish (US)
Pages (from-to)19-27
Number of pages9
JournalJournal of Dentistry
Volume58
DOIs
StatePublished - Mar 1 2017

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Silanes
Dentin
Ammonium Compounds
Peptide Hydrolases
Cathepsin K
Collagen
Disinfectants
Matrix Metalloproteinase 2
Protease Inhibitors
Matrix Metalloproteinases
Cathepsins
Chlorhexidine
Matrix Metalloproteinase Inhibitors
Powders
Cysteine
Zinc
Enzyme-Linked Immunosorbent Assay
Calcium
Control Groups
Peptides

Keywords

  • Cysteine cathepsins
  • Dentin
  • Matrix metalloproteinases
  • Quaternary ammonium silane

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Effect of a novel quaternary ammonium silane on dentin protease activities. / Umer, D.; Yiu, C. K.Y.; Burrow, M. F.; Niu, L. N.; Tay, Franklin Chi Meng.

In: Journal of Dentistry, Vol. 58, 01.03.2017, p. 19-27.

Research output: Contribution to journalArticle

Umer, D. ; Yiu, C. K.Y. ; Burrow, M. F. ; Niu, L. N. ; Tay, Franklin Chi Meng. / Effect of a novel quaternary ammonium silane on dentin protease activities. In: Journal of Dentistry. 2017 ; Vol. 58. pp. 19-27.
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abstract = "Objectives Demineralized dentin collagen release C-terminal cross-linked telopeptide (ICTP) and C-terminal peptide (CTX) during degradation. The present study evaluated the effects of dentin pre-treatment with K21, a quaternary ammonium silane (QAS), on matrix metalloproteinase (MMP) and cathepsin K-mediated collagen degradation. Methods Dentin beams were demineralized with 10{\%} H3PO4 for 24 h. After baseline dry mass measurements, the beams were divided into 5 groups (N = 10) according to protease inhibitors. The beams were pre-treated for 2 min with 2{\%} chlorhexidine (CHX), 2{\%}, 5{\%} or 10{\%} QAS; no pre-treatment was performed for the control group. The beams were subsequently incubated in calcium- and zinc-containing medium for 3, 7 or 14 days, after which changes in dry mass were measured and incubation media were examined for ICTP and CTX release. The MMP-2 and cathepsin K activities in QAS-treated dentin powder were also quantified using ELISA. Results The two factors (disinfectants and time) had a significant effect on dry mass loss, ICTP and CTX release (p < 0.001). The percentage of dry mass loss increased with time and was significantly lower in all experimental groups when compared to the control at 14 days (p < 0.001). Conversely, the rate of ICTP and CTX release was significantly lower in the experimental groups, compared to the uninhibited control at 7 and 14 days (p < 0.001). Dentinal MMP-2 and cathepsin K activities were significantly reduced after demineralized dentin was pre-treated with QAS. Conclusion The experimental QAS is a good inhibitor of MMP and cathepsin K activities in demineralized dentin. Clinical significance The newly developed antibacterial quaternary ammonium silane increases the resistance of dentin collagen to degradation by inhibiting endogenous matrix metalloproteinases and cysteine cathepsins. The quaternary ammonium silane cavity disinfectant is promising for use as a protease inhibitor to improve durability of resin-dentin bonds.",
keywords = "Cysteine cathepsins, Dentin, Matrix metalloproteinases, Quaternary ammonium silane",
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T1 - Effect of a novel quaternary ammonium silane on dentin protease activities

AU - Umer, D.

AU - Yiu, C. K.Y.

AU - Burrow, M. F.

AU - Niu, L. N.

AU - Tay, Franklin Chi Meng

PY - 2017/3/1

Y1 - 2017/3/1

N2 - Objectives Demineralized dentin collagen release C-terminal cross-linked telopeptide (ICTP) and C-terminal peptide (CTX) during degradation. The present study evaluated the effects of dentin pre-treatment with K21, a quaternary ammonium silane (QAS), on matrix metalloproteinase (MMP) and cathepsin K-mediated collagen degradation. Methods Dentin beams were demineralized with 10% H3PO4 for 24 h. After baseline dry mass measurements, the beams were divided into 5 groups (N = 10) according to protease inhibitors. The beams were pre-treated for 2 min with 2% chlorhexidine (CHX), 2%, 5% or 10% QAS; no pre-treatment was performed for the control group. The beams were subsequently incubated in calcium- and zinc-containing medium for 3, 7 or 14 days, after which changes in dry mass were measured and incubation media were examined for ICTP and CTX release. The MMP-2 and cathepsin K activities in QAS-treated dentin powder were also quantified using ELISA. Results The two factors (disinfectants and time) had a significant effect on dry mass loss, ICTP and CTX release (p < 0.001). The percentage of dry mass loss increased with time and was significantly lower in all experimental groups when compared to the control at 14 days (p < 0.001). Conversely, the rate of ICTP and CTX release was significantly lower in the experimental groups, compared to the uninhibited control at 7 and 14 days (p < 0.001). Dentinal MMP-2 and cathepsin K activities were significantly reduced after demineralized dentin was pre-treated with QAS. Conclusion The experimental QAS is a good inhibitor of MMP and cathepsin K activities in demineralized dentin. Clinical significance The newly developed antibacterial quaternary ammonium silane increases the resistance of dentin collagen to degradation by inhibiting endogenous matrix metalloproteinases and cysteine cathepsins. The quaternary ammonium silane cavity disinfectant is promising for use as a protease inhibitor to improve durability of resin-dentin bonds.

AB - Objectives Demineralized dentin collagen release C-terminal cross-linked telopeptide (ICTP) and C-terminal peptide (CTX) during degradation. The present study evaluated the effects of dentin pre-treatment with K21, a quaternary ammonium silane (QAS), on matrix metalloproteinase (MMP) and cathepsin K-mediated collagen degradation. Methods Dentin beams were demineralized with 10% H3PO4 for 24 h. After baseline dry mass measurements, the beams were divided into 5 groups (N = 10) according to protease inhibitors. The beams were pre-treated for 2 min with 2% chlorhexidine (CHX), 2%, 5% or 10% QAS; no pre-treatment was performed for the control group. The beams were subsequently incubated in calcium- and zinc-containing medium for 3, 7 or 14 days, after which changes in dry mass were measured and incubation media were examined for ICTP and CTX release. The MMP-2 and cathepsin K activities in QAS-treated dentin powder were also quantified using ELISA. Results The two factors (disinfectants and time) had a significant effect on dry mass loss, ICTP and CTX release (p < 0.001). The percentage of dry mass loss increased with time and was significantly lower in all experimental groups when compared to the control at 14 days (p < 0.001). Conversely, the rate of ICTP and CTX release was significantly lower in the experimental groups, compared to the uninhibited control at 7 and 14 days (p < 0.001). Dentinal MMP-2 and cathepsin K activities were significantly reduced after demineralized dentin was pre-treated with QAS. Conclusion The experimental QAS is a good inhibitor of MMP and cathepsin K activities in demineralized dentin. Clinical significance The newly developed antibacterial quaternary ammonium silane increases the resistance of dentin collagen to degradation by inhibiting endogenous matrix metalloproteinases and cysteine cathepsins. The quaternary ammonium silane cavity disinfectant is promising for use as a protease inhibitor to improve durability of resin-dentin bonds.

KW - Cysteine cathepsins

KW - Dentin

KW - Matrix metalloproteinases

KW - Quaternary ammonium silane

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