Effect of ANP on sustained aldosterone secretion stimulated by angiotensin II

Carlos M Isales, Wendy B Bollag, L. C. Kiernan, P. Q. Barrett

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The sustained aldosterone secretory response to angiotensin II (ANG II) depends on receptor-mediated increases in membrane diglyceride (DG) and an increase in calcium influx rate. These signals serve to activate membrane-associated protein kinase C (PKC) and result in enhanced phosphorylation of a unique set of proteins. These events can be mimicked by the addition of a phorbol ester, 12-O-tetra decanoyl phorbol 13-acetate (TPA), and a calcium ionophore, A23187, that bypass the initial receptor-associated events. We studied the inhibitory action of atrial natriuretic peptide (4-28 hANP) on the sustained secretory response to ANG II in isolated bovine adrenal glomerulosa cells. Although 10 nM ANP inhibited aldosterone secretion, it did not significantly alter the ANG II-elicited rise in 45Ca2+ influx rate [control (CON): 0.44 ± 0.06; ANG II: 1.11 ± 0.12 (P<0.001); ANG II + ANP: 1.18 ± 0.14], the steady-state level of aequorin luminescence [intracellular [Ca2+] ([Ca2+](i))], or the rise in cellular DG content [CON: 0.132 ± 0.01; ANG II: 0.194 ± 0.01 (P<0.005); ANG II + ANP: 0.202 ± 0.01 nmol/106 cells]. In addition, ANP was able to inhibit aldosterone secretion stimulated by the combined addition of A23187 + TPA. When protein phosphorylation in the ANP-inhibited cells was evaluated, ANG II-induced protein phosphorylation events were preserved. In contrast to the effect of ANP, the calcium channel blocker nitrendipine abolished the ANG II-induced rise in 45Ca2+ influx rate, reduced the steady-state level of [Ca2+](i), and returned the phosphoproteins to their control states. Taken together, these data suggest that the mechanism of ANP inhibition is not via an effect on ANG II-elicited second messenger generation and probably lies beyond the activation of PKC.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume256
Issue number1
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

Atrial Natriuretic Factor
Aldosterone
Angiotensin II
Phosphorylation
Diglycerides
Calcimycin
Protein Kinase C
Aequorin
Zona Glomerulosa
Nitrendipine
Proteins
Calcium Ionophores
Phosphoproteins
Calcium Channel Blockers
Second Messenger Systems
Phorbol Esters
Luminescence
Membrane Proteins
Acetates
Chemical activation

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Effect of ANP on sustained aldosterone secretion stimulated by angiotensin II. / Isales, Carlos M; Bollag, Wendy B; Kiernan, L. C.; Barrett, P. Q.

In: American Journal of Physiology - Cell Physiology, Vol. 256, No. 1, 01.01.1989.

Research output: Contribution to journalArticle

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