TY - JOUR
T1 - Effect of antiglycolipid antisera on the lipid composition of cultured mouse spinal cords
AU - Saito, Megumi
AU - Macala, Lawrence J.
AU - Roth, German A.
AU - Bornstein, Murray B.
AU - Yu, Robert K.
N1 - Funding Information:
’ This work was supported by National Multiple Sclerosis Society grant RG 1289-C-4 and National Institutes of Health grants NS-I 1853. NS-23102, and NS-I 1920. Dr. Roth and Dr. Bomstein are at Albert Einstein College of Medicine and Dr. Saito, Dr. Macala, and Dr. Yu are at Yale University School of Medicine, where reprint requests should be addressed to Dr. Yu.
PY - 1986/6
Y1 - 1986/6
N2 - To examine the effect of antiglycolipid antibodies on demyelination, myelinated cultures of embryonic mouse spinal cords were treated by antigalactocerebroside (anti-GC), anti-GM1; and anti-GM4 antisera, and the lipid composition of the cultures were studied. The anti-GC antiserum-treated cultures, which exhibited severe morphologic signs of demyelination, revealed a significant reduction of cerebroside. The anti-GM4 or anti-GM1 antiserum-treated cultures, which exhibited mild degrees of demyelination, also had low contents of cerebroside. These results support our previous data showing that antiglycolipid antibodies cause demyelination in cultured mouse spinal cords, and suggest a possible role of myelin-specific glycolipids in the demyelination process.
AB - To examine the effect of antiglycolipid antibodies on demyelination, myelinated cultures of embryonic mouse spinal cords were treated by antigalactocerebroside (anti-GC), anti-GM1; and anti-GM4 antisera, and the lipid composition of the cultures were studied. The anti-GC antiserum-treated cultures, which exhibited severe morphologic signs of demyelination, revealed a significant reduction of cerebroside. The anti-GM4 or anti-GM1 antiserum-treated cultures, which exhibited mild degrees of demyelination, also had low contents of cerebroside. These results support our previous data showing that antiglycolipid antibodies cause demyelination in cultured mouse spinal cords, and suggest a possible role of myelin-specific glycolipids in the demyelination process.
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U2 - 10.1016/0014-4886(86)90314-6
DO - 10.1016/0014-4886(86)90314-6
M3 - Article
C2 - 3709746
AN - SCOPUS:0022486216
SN - 0014-4886
VL - 92
SP - 752
EP - 756
JO - Experimental Neurology
JF - Experimental Neurology
IS - 3
ER -