Effect of bone morphogenetic protein-2, demineralized bone matrix and systemic parathyroid hormone (1-34) on local bone formation in a rat calvaria critical-size defect model

B. W. Stancoven, J. Lee, D. R. Dixon, J. C. Mcpherson, F. C. Bisch, U. M E Wikesjö, C. Susin

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Abstract

Aim: To determine the potential of recombinant human bone morphogenetic protein-2 (rhBMP-2) soak-loaded on to an absorbable collagen sponge (ACS) to induce local bone formation compared with the clinical reference demineralized bone matrix (DBM) and to investigate potential additive/synergistic effects of exogenous parathyroid hormone (PTH). Methods: Critical-size (8mm), through-through calvaria osteotomy defects in 160 adult male Sprague-Dawley rats were randomized to receive one of eight interventions: rhBMP-2/ACS, DBM, ACS, or serve as controls (empty defects) combined or not with systemic PTH. Ten animals from each group were followed for 4 and 8wks for radiographic and histometric analysis. Multivariable analysis was used to assess the effect of experimental intervention and healing time on local bone formation. Results: In the multivariable analysis, rhBMP-2/ACS exhibited significantly greater histologic bone formation than control (β±SE: 54.76±5.85, p<0.001) and ACS (β±SE: 9.14±3.31, p=0.007) whereas DBM showed significantly less bone formation than control (β±SE: -32.32±8.23, p<0.001). Overall, PTH did not show a significant effect on bone formation (β±SE: 2.72±6.91, p=0.70). No significant differences in histological defect closure were observed between 4 and 8wks for all but the control group without PTH. Conclusion: rhBMP-2/ACS significantly stimulates local bone formation whereas bone formation appears significantly limited by DBM. Systemic application of PTH provided no discernible additive/synergistic effects on local bone formation.

Original languageEnglish (US)
Pages (from-to)243-251
Number of pages9
JournalJournal of Periodontal Research
Volume48
Issue number2
DOIs
StatePublished - Apr 1 2013

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Keywords

  • Bone formation
  • Demineralized bone matrix
  • PTH
  • RhBMP-2
  • Tissue engineering

ASJC Scopus subject areas

  • Periodontics

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