Abstract
Objective Recent studies supported the use of protein cross-linking agents during bonding procedures to inactivate endogenous dentin proteases, preventing dentin collagen degradation thus improving bond durability. The aim of this study was to evaluate the effect of a 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-containing conditioner on the stability of the adhesive interface created by two etch-and-rinse adhesives. Methods Human dentin was etched with 35% phosphoric acid, treated with 0.3 M EDC-containing conditioner followed by a three-step or a two-step etch-and-rinse adhesive. Adhesives were applied to control specimens without EDC pre-treatment. Specimens were subjected to microtensile bond strength test and pulled to failure after 24 h or 1 year of storage and interfacial nanoleakage expression was evaluated and quantified by light microscopy. Additionally, to investigate endogenous dentin matrix metalloproteinase activity a zymographic assay was performed on protein extracts obtained from phosphoric-acid-etched dentin powder with or without EDC treatment. Results The use of the EDC-containing conditioner did not affect immediate bond strength to dentin but contributed to preserve the bond strength after 1 year (p < 0.05) for both tested adhesives. No difference was found in the interfacial nanoleakage expression that increased after aging irrespective from the treatment. EDC pre-treatment inhibited dentin endogenous MMPs as assayed with the zymography. Significance In conclusion, the results of the study provide proof that EDC can produce long-term inactivation of MMPs in acid-etched dentin matrices contributing to bond strength preservation over time. Future studies are needed to support the use of EDC in vivo.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1040-1047 |
| Number of pages | 8 |
| Journal | Dental Materials |
| Volume | 29 |
| Issue number | 10 |
| DOIs | |
| State | Published - Oct 1 2013 |
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Keywords
- Dentin bonding systems
- EDC
- Enzymatic degradation
- MMPs
- Protein cross-linkers
ASJC Scopus subject areas
- Materials Science(all)
- Dentistry(all)
- Mechanics of Materials
Cite this
Effect of carbodiimide (EDC) on the bond stability of etch-and-rinse adhesive systems. / Mazzoni, Annalisa; Angeloni, Valeria; Apolonio, Fabianni M.; Scotti, Nicola; Tjäderhane, Leo; Tezvergil-Mutluay, Arzu; Di Lenarda, Roberto; Tay, Franklin Chi Meng; Pashley, David Henry; Breschi, Lorenzo.
In: Dental Materials, Vol. 29, No. 10, 01.10.2013, p. 1040-1047.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of carbodiimide (EDC) on the bond stability of etch-and-rinse adhesive systems
AU - Mazzoni, Annalisa
AU - Angeloni, Valeria
AU - Apolonio, Fabianni M.
AU - Scotti, Nicola
AU - Tjäderhane, Leo
AU - Tezvergil-Mutluay, Arzu
AU - Di Lenarda, Roberto
AU - Tay, Franklin Chi Meng
AU - Pashley, David Henry
AU - Breschi, Lorenzo
PY - 2013/10/1
Y1 - 2013/10/1
N2 - Objective Recent studies supported the use of protein cross-linking agents during bonding procedures to inactivate endogenous dentin proteases, preventing dentin collagen degradation thus improving bond durability. The aim of this study was to evaluate the effect of a 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-containing conditioner on the stability of the adhesive interface created by two etch-and-rinse adhesives. Methods Human dentin was etched with 35% phosphoric acid, treated with 0.3 M EDC-containing conditioner followed by a three-step or a two-step etch-and-rinse adhesive. Adhesives were applied to control specimens without EDC pre-treatment. Specimens were subjected to microtensile bond strength test and pulled to failure after 24 h or 1 year of storage and interfacial nanoleakage expression was evaluated and quantified by light microscopy. Additionally, to investigate endogenous dentin matrix metalloproteinase activity a zymographic assay was performed on protein extracts obtained from phosphoric-acid-etched dentin powder with or without EDC treatment. Results The use of the EDC-containing conditioner did not affect immediate bond strength to dentin but contributed to preserve the bond strength after 1 year (p < 0.05) for both tested adhesives. No difference was found in the interfacial nanoleakage expression that increased after aging irrespective from the treatment. EDC pre-treatment inhibited dentin endogenous MMPs as assayed with the zymography. Significance In conclusion, the results of the study provide proof that EDC can produce long-term inactivation of MMPs in acid-etched dentin matrices contributing to bond strength preservation over time. Future studies are needed to support the use of EDC in vivo.
AB - Objective Recent studies supported the use of protein cross-linking agents during bonding procedures to inactivate endogenous dentin proteases, preventing dentin collagen degradation thus improving bond durability. The aim of this study was to evaluate the effect of a 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-containing conditioner on the stability of the adhesive interface created by two etch-and-rinse adhesives. Methods Human dentin was etched with 35% phosphoric acid, treated with 0.3 M EDC-containing conditioner followed by a three-step or a two-step etch-and-rinse adhesive. Adhesives were applied to control specimens without EDC pre-treatment. Specimens were subjected to microtensile bond strength test and pulled to failure after 24 h or 1 year of storage and interfacial nanoleakage expression was evaluated and quantified by light microscopy. Additionally, to investigate endogenous dentin matrix metalloproteinase activity a zymographic assay was performed on protein extracts obtained from phosphoric-acid-etched dentin powder with or without EDC treatment. Results The use of the EDC-containing conditioner did not affect immediate bond strength to dentin but contributed to preserve the bond strength after 1 year (p < 0.05) for both tested adhesives. No difference was found in the interfacial nanoleakage expression that increased after aging irrespective from the treatment. EDC pre-treatment inhibited dentin endogenous MMPs as assayed with the zymography. Significance In conclusion, the results of the study provide proof that EDC can produce long-term inactivation of MMPs in acid-etched dentin matrices contributing to bond strength preservation over time. Future studies are needed to support the use of EDC in vivo.
KW - Dentin bonding systems
KW - EDC
KW - Enzymatic degradation
KW - MMPs
KW - Protein cross-linkers
UR - http://www.scopus.com/inward/record.url?scp=84884135275&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84884135275&partnerID=8YFLogxK
U2 - 10.1016/j.dental.2013.07.010
DO - 10.1016/j.dental.2013.07.010
M3 - Article
C2 - 23916318
AN - SCOPUS:84884135275
VL - 29
SP - 1040
EP - 1047
JO - Dental Materials
JF - Dental Materials
SN - 0109-5641
IS - 10
ER -