Effect of carbodiimide (EDC) on the bond stability of etch-and-rinse adhesive systems

Annalisa Mazzoni, Valeria Angeloni, Fabianni M. Apolonio, Nicola Scotti, Leo Tjäderhane, Arzu Tezvergil-Mutluay, Roberto Di Lenarda, Franklin Chi Meng Tay, David Henry Pashley, Lorenzo Breschi

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Objective Recent studies supported the use of protein cross-linking agents during bonding procedures to inactivate endogenous dentin proteases, preventing dentin collagen degradation thus improving bond durability. The aim of this study was to evaluate the effect of a 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-containing conditioner on the stability of the adhesive interface created by two etch-and-rinse adhesives. Methods Human dentin was etched with 35% phosphoric acid, treated with 0.3 M EDC-containing conditioner followed by a three-step or a two-step etch-and-rinse adhesive. Adhesives were applied to control specimens without EDC pre-treatment. Specimens were subjected to microtensile bond strength test and pulled to failure after 24 h or 1 year of storage and interfacial nanoleakage expression was evaluated and quantified by light microscopy. Additionally, to investigate endogenous dentin matrix metalloproteinase activity a zymographic assay was performed on protein extracts obtained from phosphoric-acid-etched dentin powder with or without EDC treatment. Results The use of the EDC-containing conditioner did not affect immediate bond strength to dentin but contributed to preserve the bond strength after 1 year (p < 0.05) for both tested adhesives. No difference was found in the interfacial nanoleakage expression that increased after aging irrespective from the treatment. EDC pre-treatment inhibited dentin endogenous MMPs as assayed with the zymography. Significance In conclusion, the results of the study provide proof that EDC can produce long-term inactivation of MMPs in acid-etched dentin matrices contributing to bond strength preservation over time. Future studies are needed to support the use of EDC in vivo.

Original languageEnglish (US)
Pages (from-to)1040-1047
Number of pages8
JournalDental Materials
Volume29
Issue number10
DOIs
StatePublished - Oct 1 2013

Fingerprint

Dentin
Adhesives
Matrix Metalloproteinases
Phosphoric acid
Ethyldimethylaminopropyl Carbodiimide
Proteins
Collagen
Powders
Optical microscopy
Assays
Durability
Peptide Hydrolases
Aging of materials
1-ethyl-3-(3-(diethylamino)propyl)carbodiimide
Degradation
Acids
Microscopy
Light
phosphoric acid

Keywords

  • Dentin bonding systems
  • EDC
  • Enzymatic degradation
  • MMPs
  • Protein cross-linkers

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Mazzoni, A., Angeloni, V., Apolonio, F. M., Scotti, N., Tjäderhane, L., Tezvergil-Mutluay, A., ... Breschi, L. (2013). Effect of carbodiimide (EDC) on the bond stability of etch-and-rinse adhesive systems. Dental Materials, 29(10), 1040-1047. https://doi.org/10.1016/j.dental.2013.07.010

Effect of carbodiimide (EDC) on the bond stability of etch-and-rinse adhesive systems. / Mazzoni, Annalisa; Angeloni, Valeria; Apolonio, Fabianni M.; Scotti, Nicola; Tjäderhane, Leo; Tezvergil-Mutluay, Arzu; Di Lenarda, Roberto; Tay, Franklin Chi Meng; Pashley, David Henry; Breschi, Lorenzo.

In: Dental Materials, Vol. 29, No. 10, 01.10.2013, p. 1040-1047.

Research output: Contribution to journalArticle

Mazzoni, A, Angeloni, V, Apolonio, FM, Scotti, N, Tjäderhane, L, Tezvergil-Mutluay, A, Di Lenarda, R, Tay, FCM, Pashley, DH & Breschi, L 2013, 'Effect of carbodiimide (EDC) on the bond stability of etch-and-rinse adhesive systems', Dental Materials, vol. 29, no. 10, pp. 1040-1047. https://doi.org/10.1016/j.dental.2013.07.010
Mazzoni A, Angeloni V, Apolonio FM, Scotti N, Tjäderhane L, Tezvergil-Mutluay A et al. Effect of carbodiimide (EDC) on the bond stability of etch-and-rinse adhesive systems. Dental Materials. 2013 Oct 1;29(10):1040-1047. https://doi.org/10.1016/j.dental.2013.07.010
Mazzoni, Annalisa ; Angeloni, Valeria ; Apolonio, Fabianni M. ; Scotti, Nicola ; Tjäderhane, Leo ; Tezvergil-Mutluay, Arzu ; Di Lenarda, Roberto ; Tay, Franklin Chi Meng ; Pashley, David Henry ; Breschi, Lorenzo. / Effect of carbodiimide (EDC) on the bond stability of etch-and-rinse adhesive systems. In: Dental Materials. 2013 ; Vol. 29, No. 10. pp. 1040-1047.
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abstract = "Objective Recent studies supported the use of protein cross-linking agents during bonding procedures to inactivate endogenous dentin proteases, preventing dentin collagen degradation thus improving bond durability. The aim of this study was to evaluate the effect of a 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-containing conditioner on the stability of the adhesive interface created by two etch-and-rinse adhesives. Methods Human dentin was etched with 35{\%} phosphoric acid, treated with 0.3 M EDC-containing conditioner followed by a three-step or a two-step etch-and-rinse adhesive. Adhesives were applied to control specimens without EDC pre-treatment. Specimens were subjected to microtensile bond strength test and pulled to failure after 24 h or 1 year of storage and interfacial nanoleakage expression was evaluated and quantified by light microscopy. Additionally, to investigate endogenous dentin matrix metalloproteinase activity a zymographic assay was performed on protein extracts obtained from phosphoric-acid-etched dentin powder with or without EDC treatment. Results The use of the EDC-containing conditioner did not affect immediate bond strength to dentin but contributed to preserve the bond strength after 1 year (p < 0.05) for both tested adhesives. No difference was found in the interfacial nanoleakage expression that increased after aging irrespective from the treatment. EDC pre-treatment inhibited dentin endogenous MMPs as assayed with the zymography. Significance In conclusion, the results of the study provide proof that EDC can produce long-term inactivation of MMPs in acid-etched dentin matrices contributing to bond strength preservation over time. Future studies are needed to support the use of EDC in vivo.",
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AU - Mazzoni, Annalisa

AU - Angeloni, Valeria

AU - Apolonio, Fabianni M.

AU - Scotti, Nicola

AU - Tjäderhane, Leo

AU - Tezvergil-Mutluay, Arzu

AU - Di Lenarda, Roberto

AU - Tay, Franklin Chi Meng

AU - Pashley, David Henry

AU - Breschi, Lorenzo

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N2 - Objective Recent studies supported the use of protein cross-linking agents during bonding procedures to inactivate endogenous dentin proteases, preventing dentin collagen degradation thus improving bond durability. The aim of this study was to evaluate the effect of a 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-containing conditioner on the stability of the adhesive interface created by two etch-and-rinse adhesives. Methods Human dentin was etched with 35% phosphoric acid, treated with 0.3 M EDC-containing conditioner followed by a three-step or a two-step etch-and-rinse adhesive. Adhesives were applied to control specimens without EDC pre-treatment. Specimens were subjected to microtensile bond strength test and pulled to failure after 24 h or 1 year of storage and interfacial nanoleakage expression was evaluated and quantified by light microscopy. Additionally, to investigate endogenous dentin matrix metalloproteinase activity a zymographic assay was performed on protein extracts obtained from phosphoric-acid-etched dentin powder with or without EDC treatment. Results The use of the EDC-containing conditioner did not affect immediate bond strength to dentin but contributed to preserve the bond strength after 1 year (p < 0.05) for both tested adhesives. No difference was found in the interfacial nanoleakage expression that increased after aging irrespective from the treatment. EDC pre-treatment inhibited dentin endogenous MMPs as assayed with the zymography. Significance In conclusion, the results of the study provide proof that EDC can produce long-term inactivation of MMPs in acid-etched dentin matrices contributing to bond strength preservation over time. Future studies are needed to support the use of EDC in vivo.

AB - Objective Recent studies supported the use of protein cross-linking agents during bonding procedures to inactivate endogenous dentin proteases, preventing dentin collagen degradation thus improving bond durability. The aim of this study was to evaluate the effect of a 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-containing conditioner on the stability of the adhesive interface created by two etch-and-rinse adhesives. Methods Human dentin was etched with 35% phosphoric acid, treated with 0.3 M EDC-containing conditioner followed by a three-step or a two-step etch-and-rinse adhesive. Adhesives were applied to control specimens without EDC pre-treatment. Specimens were subjected to microtensile bond strength test and pulled to failure after 24 h or 1 year of storage and interfacial nanoleakage expression was evaluated and quantified by light microscopy. Additionally, to investigate endogenous dentin matrix metalloproteinase activity a zymographic assay was performed on protein extracts obtained from phosphoric-acid-etched dentin powder with or without EDC treatment. Results The use of the EDC-containing conditioner did not affect immediate bond strength to dentin but contributed to preserve the bond strength after 1 year (p < 0.05) for both tested adhesives. No difference was found in the interfacial nanoleakage expression that increased after aging irrespective from the treatment. EDC pre-treatment inhibited dentin endogenous MMPs as assayed with the zymography. Significance In conclusion, the results of the study provide proof that EDC can produce long-term inactivation of MMPs in acid-etched dentin matrices contributing to bond strength preservation over time. Future studies are needed to support the use of EDC in vivo.

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