Effect of contrast material on transitional cell carcinoma viability

Kelly A. Lindert, Martha Kennedy Terris

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objectives. To assess the effects of contrast material on the viability of transitional cell carcinoma (TCC) cells and the ability of such cells to attach to a recipient bed, because seeding of TCC into the upper urinary tract is a possibility during retrograde pyelography or percutaneous procedures.Methods. Primary cultures of TCC cells were established and placed in either quarter, half, or full-strength contrast for 10 minutes or one-quarter strength contrast for 10, 30, and 60 minutes. Cells were then removed from the contrast agent, resuspended in urothelium-specific media, and incubated for 5 days, after which the cells were counted.Results. A pronounced decrease in cell viability was observed with increasing exposure time and contrast material concentration. Cells incubated for 10, 30, and 60 minutes with contrast yielded an average of 79%, 60%, and 12% of the control group growth, respectively (P <0.001). Likewise, plates incubated with quarter, half, and full-strength contrast yielded 79%, 27%, and 10% of the control group growth, respectively (P <0.001). The difference in the response of low-grade superficial and high-grade invasive bladder tumors was not statistically significant.Conclusions. TCC cells that have been exposed to dilute contrast material for a short period are able to attach and grow on an adequate recipient bed. However, increasing the contrast concentration and/or the exposure time appears to decrease the viability and adherence of the TCC cells. Copyright (C) 2000 Elsevier Science Inc.

Original languageEnglish (US)
Pages (from-to)876-879
Number of pages4
JournalUrology
Volume56
Issue number5
DOIs
StatePublished - Nov 8 2000
Externally publishedYes

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Transitional Cell Carcinoma
Contrast Media
Cell Survival
Urothelium
Control Groups
Urography
Growth
Urinary Tract
Urinary Bladder Neoplasms

ASJC Scopus subject areas

  • Urology

Cite this

Effect of contrast material on transitional cell carcinoma viability. / Lindert, Kelly A.; Terris, Martha Kennedy.

In: Urology, Vol. 56, No. 5, 08.11.2000, p. 876-879.

Research output: Contribution to journalArticle

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abstract = "Objectives. To assess the effects of contrast material on the viability of transitional cell carcinoma (TCC) cells and the ability of such cells to attach to a recipient bed, because seeding of TCC into the upper urinary tract is a possibility during retrograde pyelography or percutaneous procedures.Methods. Primary cultures of TCC cells were established and placed in either quarter, half, or full-strength contrast for 10 minutes or one-quarter strength contrast for 10, 30, and 60 minutes. Cells were then removed from the contrast agent, resuspended in urothelium-specific media, and incubated for 5 days, after which the cells were counted.Results. A pronounced decrease in cell viability was observed with increasing exposure time and contrast material concentration. Cells incubated for 10, 30, and 60 minutes with contrast yielded an average of 79{\%}, 60{\%}, and 12{\%} of the control group growth, respectively (P <0.001). Likewise, plates incubated with quarter, half, and full-strength contrast yielded 79{\%}, 27{\%}, and 10{\%} of the control group growth, respectively (P <0.001). The difference in the response of low-grade superficial and high-grade invasive bladder tumors was not statistically significant.Conclusions. TCC cells that have been exposed to dilute contrast material for a short period are able to attach and grow on an adequate recipient bed. However, increasing the contrast concentration and/or the exposure time appears to decrease the viability and adherence of the TCC cells. Copyright (C) 2000 Elsevier Science Inc.",
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