Effect of curcumin on pro-angiogenic factors in the xenograft model of breast cancer

Lívia Carvalho Ferreira, Ali Syed Arbab, Bruna Victorasso Jardim-Perassi, Thaiz Ferraz Borin, Nadimpalli R.S. Varma, A. S.M. Iskander, Adarsh Shankar, Meser M. Ali, Debora Aparecida Pires de Campos Zuccari

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The formation of a new blood vessel is stimulated by angiogenic factors. Curcumin, which is the active ingredient of the spice plant Curcuma longa L and is used as food and traditional medicine, has shown anticancer effects against different types of cancers. We evaluated the effects of curcumin on angiogenesis/pro-angiogenic factors in a mouse model of human breast cancer. Cell viability was measured by the MTT assay after curcumin treatment in triple-negative breast cancer cells (MDA-MB-231). For the in vivo study, human breast cancer was induced in athymic mice and treated with 300 mg/kg/day of curcumin administered intraperitoneally. Tumor size was measured weekly, and the animals underwent single photon emission computed tomography (SPECT) scanning with Tc-99m tagged VEGF-c to detect the in vivo expression of VEGFR2/3. In addition, the expression of proangiogenic/ growth factors in the tumor extracts was evaluated by a membrane antibody array. Histological analysis was performed to confirm the effect of curcumin on neovascularization. The MTT assay showed that curcumin significantly reduced the cell viability of MDA-MB-231 cells. In breast cancer xenografts, curcumin treatment led to a decrease in tumor volume and cell proliferation (Ki-67) compared with the vehicle treated group. Tc-99m-HYNIC-VEGF-c-SPECT imaging showed decreased uptake to the tumor, which may indicate a lower expression of VEGFR2/3 in curcumin treated tumors; however, a statistically significant difference was not achieved (p>0.05). Additionally, curcumin treatment showed a significantly low level of expression of pro-angiogenic factors (p<0.05) and a decrease in micro-vessel density (vWF) in animals compared with that of vehicle treated tumors. In conclusion, curcumin treatment showed effectiveness in reducing tumor growth and cell proliferation, as well as in the inhibition of angiogenesis

Original languageEnglish (US)
Pages (from-to)1285-1296
Number of pages12
JournalAnti-Cancer Agents in Medicinal Chemistry
Volume15
Issue number10
DOIs
StatePublished - Dec 1 2015

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Curcumin
Angiogenesis Inducing Agents
Heterografts
Breast Neoplasms
Neoplasms
Single-Photon Emission-Computed Tomography
Cell Survival
Cell Proliferation
Triple Negative Breast Neoplasms
Curcuma
Spices
Traditional Medicine
Tumor Burden
Nude Mice
Vascular Endothelial Growth Factor A
Blood Vessels
Intercellular Signaling Peptides and Proteins
Therapeutics
Food

Keywords

  • Angiogenesis
  • Breast neoplasm
  • Curcumin
  • SPECT
  • Xenograft model

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology
  • Cancer Research

Cite this

Ferreira, L. C., Arbab, A. S., Jardim-Perassi, B. V., Borin, T. F., Varma, N. R. S., Iskander, A. S. M., ... de Campos Zuccari, D. A. P. (2015). Effect of curcumin on pro-angiogenic factors in the xenograft model of breast cancer. Anti-Cancer Agents in Medicinal Chemistry, 15(10), 1285-1296. https://doi.org/10.2174/1871520615666150520093644

Effect of curcumin on pro-angiogenic factors in the xenograft model of breast cancer. / Ferreira, Lívia Carvalho; Arbab, Ali Syed; Jardim-Perassi, Bruna Victorasso; Borin, Thaiz Ferraz; Varma, Nadimpalli R.S.; Iskander, A. S.M.; Shankar, Adarsh; Ali, Meser M.; de Campos Zuccari, Debora Aparecida Pires.

In: Anti-Cancer Agents in Medicinal Chemistry, Vol. 15, No. 10, 01.12.2015, p. 1285-1296.

Research output: Contribution to journalArticle

Ferreira, LC, Arbab, AS, Jardim-Perassi, BV, Borin, TF, Varma, NRS, Iskander, ASM, Shankar, A, Ali, MM & de Campos Zuccari, DAP 2015, 'Effect of curcumin on pro-angiogenic factors in the xenograft model of breast cancer', Anti-Cancer Agents in Medicinal Chemistry, vol. 15, no. 10, pp. 1285-1296. https://doi.org/10.2174/1871520615666150520093644
Ferreira, Lívia Carvalho ; Arbab, Ali Syed ; Jardim-Perassi, Bruna Victorasso ; Borin, Thaiz Ferraz ; Varma, Nadimpalli R.S. ; Iskander, A. S.M. ; Shankar, Adarsh ; Ali, Meser M. ; de Campos Zuccari, Debora Aparecida Pires. / Effect of curcumin on pro-angiogenic factors in the xenograft model of breast cancer. In: Anti-Cancer Agents in Medicinal Chemistry. 2015 ; Vol. 15, No. 10. pp. 1285-1296.
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