Effect of cyclic loading under enzymatic activity on resin-dentin interfaces of two self-etching adhesives

Tissiana Bortolotto, Ioana Onisor, Ivo Krejci, Marco Ferrari, Franklin R. Tay, Serge Bouillaguet

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objectives: To evaluate microtensile bond strength and micro-morphology at the resin-dentin interfaces of two self-etching adhesive systems subjected to simultaneous mechanical and enzymatic stress. Methods: Sixteen enamel/dentin discs were bonded with a two-step self-etching adhesive (AdheSE, n = 8) and a one-step self-etching adhesive (Xeno III, n = 8) to a 2 mm thick resin composite layer. One resin-dentin bar was obtained per tooth. In half of the specimens of each group μTBS and micro-morphological evaluations (TEM) was performed without loading. The other half was mechanically loaded in a cholinesterase-containing solution. μTBS as well as ultra-morphological evaluations of the directly loaded areas using TEM were performed on the loaded specimens. Results: The μTBS of the specimens (non-loaded/loaded) were of 39.6 ± 14.7/35.4 ± 22.1 and of 21.8 ± 29.8/15.9 ± 25.5 for AdheSE and Xeno III, respectively. Under TEM, both materials presented signs of nanoleakage. However, on loaded specimens the extent of nanoleakage was slightly reduced for AdheSE and no silver staining was observed on the adhesive interface of Xeno III. TEM evaluations of the specimens' loaded area revealed no decrease in the width of the adhesive interface for AdheSE. The contrary was observed in the interface created by Xeno III. Significance: The adhesive interfaces created by the two-step self-etching adhesive (AdheSE) could better withstand both mechanical and enzymatic stresses on the long-term than the one-step self-etching system (Xeno III) tested in the present study.

Original languageEnglish (US)
Pages (from-to)178-184
Number of pages7
JournalDental Materials
Volume24
Issue number2
DOIs
StatePublished - Feb 1 2008

Fingerprint

Dentin
Adhesives
Etching
Resins
Transmission electron microscopy
Mechanical Stress
Silver Staining
Enamels
Composite Resins
Cholinesterases
Dental Enamel
Silver
Tooth
Xeno III

Keywords

  • Aging
  • Biomechanics
  • Cholinesterase
  • Composite
  • Cyclic load
  • Dentin
  • Enzyme
  • Micromorphology
  • Microtensile
  • Self-etch

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Effect of cyclic loading under enzymatic activity on resin-dentin interfaces of two self-etching adhesives. / Bortolotto, Tissiana; Onisor, Ioana; Krejci, Ivo; Ferrari, Marco; Tay, Franklin R.; Bouillaguet, Serge.

In: Dental Materials, Vol. 24, No. 2, 01.02.2008, p. 178-184.

Research output: Contribution to journalArticle

Bortolotto, Tissiana ; Onisor, Ioana ; Krejci, Ivo ; Ferrari, Marco ; Tay, Franklin R. ; Bouillaguet, Serge. / Effect of cyclic loading under enzymatic activity on resin-dentin interfaces of two self-etching adhesives. In: Dental Materials. 2008 ; Vol. 24, No. 2. pp. 178-184.
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AU - Bortolotto, Tissiana

AU - Onisor, Ioana

AU - Krejci, Ivo

AU - Ferrari, Marco

AU - Tay, Franklin R.

AU - Bouillaguet, Serge

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N2 - Objectives: To evaluate microtensile bond strength and micro-morphology at the resin-dentin interfaces of two self-etching adhesive systems subjected to simultaneous mechanical and enzymatic stress. Methods: Sixteen enamel/dentin discs were bonded with a two-step self-etching adhesive (AdheSE, n = 8) and a one-step self-etching adhesive (Xeno III, n = 8) to a 2 mm thick resin composite layer. One resin-dentin bar was obtained per tooth. In half of the specimens of each group μTBS and micro-morphological evaluations (TEM) was performed without loading. The other half was mechanically loaded in a cholinesterase-containing solution. μTBS as well as ultra-morphological evaluations of the directly loaded areas using TEM were performed on the loaded specimens. Results: The μTBS of the specimens (non-loaded/loaded) were of 39.6 ± 14.7/35.4 ± 22.1 and of 21.8 ± 29.8/15.9 ± 25.5 for AdheSE and Xeno III, respectively. Under TEM, both materials presented signs of nanoleakage. However, on loaded specimens the extent of nanoleakage was slightly reduced for AdheSE and no silver staining was observed on the adhesive interface of Xeno III. TEM evaluations of the specimens' loaded area revealed no decrease in the width of the adhesive interface for AdheSE. The contrary was observed in the interface created by Xeno III. Significance: The adhesive interfaces created by the two-step self-etching adhesive (AdheSE) could better withstand both mechanical and enzymatic stresses on the long-term than the one-step self-etching system (Xeno III) tested in the present study.

AB - Objectives: To evaluate microtensile bond strength and micro-morphology at the resin-dentin interfaces of two self-etching adhesive systems subjected to simultaneous mechanical and enzymatic stress. Methods: Sixteen enamel/dentin discs were bonded with a two-step self-etching adhesive (AdheSE, n = 8) and a one-step self-etching adhesive (Xeno III, n = 8) to a 2 mm thick resin composite layer. One resin-dentin bar was obtained per tooth. In half of the specimens of each group μTBS and micro-morphological evaluations (TEM) was performed without loading. The other half was mechanically loaded in a cholinesterase-containing solution. μTBS as well as ultra-morphological evaluations of the directly loaded areas using TEM were performed on the loaded specimens. Results: The μTBS of the specimens (non-loaded/loaded) were of 39.6 ± 14.7/35.4 ± 22.1 and of 21.8 ± 29.8/15.9 ± 25.5 for AdheSE and Xeno III, respectively. Under TEM, both materials presented signs of nanoleakage. However, on loaded specimens the extent of nanoleakage was slightly reduced for AdheSE and no silver staining was observed on the adhesive interface of Xeno III. TEM evaluations of the specimens' loaded area revealed no decrease in the width of the adhesive interface for AdheSE. The contrary was observed in the interface created by Xeno III. Significance: The adhesive interfaces created by the two-step self-etching adhesive (AdheSE) could better withstand both mechanical and enzymatic stresses on the long-term than the one-step self-etching system (Xeno III) tested in the present study.

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KW - Self-etch

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