Effect of dentin bonding agents on the secretion of inflammatory mediators from macrophages

Douglas R. Rakich, John C. Wataha, Carol A Lefebvre, R. Norman Weller

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Dentin bonding agents (DBAs) have been proposed as substitutes for amalgam as root-end filling materials. The current study tested the hypothesis that certain components of DBAs could alter the secretion of cytokines from macrophages. Such alteration would likely be undesirable for healing of the periapical tissues. Human THP-1 macrophages were exposed to 2-hydroxyethyl methacrylate, 4-methacryloxyethyl trimelliate anhydride, bisphenol-gycidylmethacrylate, and urethane dimethacrylate. The secretion of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were measured with or without challenge by lipopolysaccharide (LPS). Results showed that all DBA components completely suppressed LPS-induced IL-1β and TNF-α secretion at concentrations that suppressed mitochondrial activity by 50%. In addition, 4-methacryloxyethyl trimelliate anhydride induced secretion of IL-1β, but not TNF-α, without the LPS challenge. These results indicate that DBA components may alter normal macrophage-directed inflammatory responses if the macrophages are exposed to sufficiently high concentrations of these components.

Original languageEnglish (US)
Pages (from-to)114-117
Number of pages4
JournalJournal of Endodontics
Volume25
Issue number2
DOIs
StatePublished - Jan 1 1999

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Dentin-Bonding Agents
Macrophages
Interleukin-1
Lipopolysaccharides
Tumor Necrosis Factor-alpha
Anhydrides
Periapical Tissue
Cytokines

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Effect of dentin bonding agents on the secretion of inflammatory mediators from macrophages. / Rakich, Douglas R.; Wataha, John C.; Lefebvre, Carol A; Weller, R. Norman.

In: Journal of Endodontics, Vol. 25, No. 2, 01.01.1999, p. 114-117.

Research output: Contribution to journalArticle

Rakich, Douglas R. ; Wataha, John C. ; Lefebvre, Carol A ; Weller, R. Norman. / Effect of dentin bonding agents on the secretion of inflammatory mediators from macrophages. In: Journal of Endodontics. 1999 ; Vol. 25, No. 2. pp. 114-117.
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