Effect of dentin etching and chlorhexidine application on metalloproteinase-mediated collagen degradation

Raquel Osorio, Mónica Yamauti, Estrella Osorio, María E. Ruiz-Requena, David Pashley, Franklin Tay, Manuel Toledano

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

Dentin matrix metalloproteinases (MMPs) are involved in the degradation of collagen in resin-dentin interfaces. This study evaluated whether collagen degradation can be prevented by chlorhexidine digluconate (CHX) after different dentin demineralization procedures. The demineralization of human dentin was performed with phosphoric acid (PA), EDTA or acidic monomers (Clearfil SE Bond and Xeno V). Specimens were stored (for 24h, or for 1 or 3wk) in the presence or absence of CHX. In half of the groups, active MMP-2 was incorporated into the storage solution. At the end of each storage period, the C-terminal telopeptide (ICTP) concentration (which indicates the amount of collagen degradation) was measured in the storage solution. Collagen degradation was higher in PA- and EDTA-demineralized dentin. Chlorhexidine digluconate reduced collagen degradation in these groups only for 24h. When dentin was demineralized with Clearfil SE Bond or Xeno V, collagen degradation was reduced by up to 30%, but the addition of exogenous MMP-2 significantly increased collagen degradation. In self-etchant-treated dentin, the inhibitory effect of CHX on MMPs lasted for up to 3wk. Treating dentin with EDTA, PA or self-etching agents produces enough demineralization to permit cleavage of the exposed collagen. Monomer infiltration may exert protection on demineralized collagen, probably through immobilization of MMPs. The partial inhibitory action of CHX on MMP activity produced by self-etching adhesives was prolonged compared with the short-acting PA- or EDTA-treated dentin.

Original languageEnglish (US)
Pages (from-to)79-85
Number of pages7
JournalEuropean Journal of Oral Sciences
Volume119
Issue number1
DOIs
StatePublished - Feb 1 2011

Fingerprint

Chlorhexidine
Metalloproteases
Dentin
Collagen
Matrix Metalloproteinases
Edetic Acid
Matrix Metalloproteinase 2
Immobilization
Adhesives
chlorhexidine gluconate
phosphoric acid

Keywords

  • Chlorhexidine
  • Collagen degradation
  • Dentin
  • Matrix metalloproteinases
  • Self-etching adhesives

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Effect of dentin etching and chlorhexidine application on metalloproteinase-mediated collagen degradation. / Osorio, Raquel; Yamauti, Mónica; Osorio, Estrella; Ruiz-Requena, María E.; Pashley, David; Tay, Franklin; Toledano, Manuel.

In: European Journal of Oral Sciences, Vol. 119, No. 1, 01.02.2011, p. 79-85.

Research output: Contribution to journalArticle

Osorio, Raquel ; Yamauti, Mónica ; Osorio, Estrella ; Ruiz-Requena, María E. ; Pashley, David ; Tay, Franklin ; Toledano, Manuel. / Effect of dentin etching and chlorhexidine application on metalloproteinase-mediated collagen degradation. In: European Journal of Oral Sciences. 2011 ; Vol. 119, No. 1. pp. 79-85.
@article{f0e8e1ebf7824b80a56cc78773dd1994,
title = "Effect of dentin etching and chlorhexidine application on metalloproteinase-mediated collagen degradation",
abstract = "Dentin matrix metalloproteinases (MMPs) are involved in the degradation of collagen in resin-dentin interfaces. This study evaluated whether collagen degradation can be prevented by chlorhexidine digluconate (CHX) after different dentin demineralization procedures. The demineralization of human dentin was performed with phosphoric acid (PA), EDTA or acidic monomers (Clearfil SE Bond and Xeno V). Specimens were stored (for 24h, or for 1 or 3wk) in the presence or absence of CHX. In half of the groups, active MMP-2 was incorporated into the storage solution. At the end of each storage period, the C-terminal telopeptide (ICTP) concentration (which indicates the amount of collagen degradation) was measured in the storage solution. Collagen degradation was higher in PA- and EDTA-demineralized dentin. Chlorhexidine digluconate reduced collagen degradation in these groups only for 24h. When dentin was demineralized with Clearfil SE Bond or Xeno V, collagen degradation was reduced by up to 30{\%}, but the addition of exogenous MMP-2 significantly increased collagen degradation. In self-etchant-treated dentin, the inhibitory effect of CHX on MMPs lasted for up to 3wk. Treating dentin with EDTA, PA or self-etching agents produces enough demineralization to permit cleavage of the exposed collagen. Monomer infiltration may exert protection on demineralized collagen, probably through immobilization of MMPs. The partial inhibitory action of CHX on MMP activity produced by self-etching adhesives was prolonged compared with the short-acting PA- or EDTA-treated dentin.",
keywords = "Chlorhexidine, Collagen degradation, Dentin, Matrix metalloproteinases, Self-etching adhesives",
author = "Raquel Osorio and M{\'o}nica Yamauti and Estrella Osorio and Ruiz-Requena, {Mar{\'i}a E.} and David Pashley and Franklin Tay and Manuel Toledano",
year = "2011",
month = "2",
day = "1",
doi = "10.1111/j.1600-0722.2010.00789.x",
language = "English (US)",
volume = "119",
pages = "79--85",
journal = "European Journal of Oral Sciences",
issn = "0909-8836",
publisher = "Blackwell Munksgaard",
number = "1",

}

TY - JOUR

T1 - Effect of dentin etching and chlorhexidine application on metalloproteinase-mediated collagen degradation

AU - Osorio, Raquel

AU - Yamauti, Mónica

AU - Osorio, Estrella

AU - Ruiz-Requena, María E.

AU - Pashley, David

AU - Tay, Franklin

AU - Toledano, Manuel

PY - 2011/2/1

Y1 - 2011/2/1

N2 - Dentin matrix metalloproteinases (MMPs) are involved in the degradation of collagen in resin-dentin interfaces. This study evaluated whether collagen degradation can be prevented by chlorhexidine digluconate (CHX) after different dentin demineralization procedures. The demineralization of human dentin was performed with phosphoric acid (PA), EDTA or acidic monomers (Clearfil SE Bond and Xeno V). Specimens were stored (for 24h, or for 1 or 3wk) in the presence or absence of CHX. In half of the groups, active MMP-2 was incorporated into the storage solution. At the end of each storage period, the C-terminal telopeptide (ICTP) concentration (which indicates the amount of collagen degradation) was measured in the storage solution. Collagen degradation was higher in PA- and EDTA-demineralized dentin. Chlorhexidine digluconate reduced collagen degradation in these groups only for 24h. When dentin was demineralized with Clearfil SE Bond or Xeno V, collagen degradation was reduced by up to 30%, but the addition of exogenous MMP-2 significantly increased collagen degradation. In self-etchant-treated dentin, the inhibitory effect of CHX on MMPs lasted for up to 3wk. Treating dentin with EDTA, PA or self-etching agents produces enough demineralization to permit cleavage of the exposed collagen. Monomer infiltration may exert protection on demineralized collagen, probably through immobilization of MMPs. The partial inhibitory action of CHX on MMP activity produced by self-etching adhesives was prolonged compared with the short-acting PA- or EDTA-treated dentin.

AB - Dentin matrix metalloproteinases (MMPs) are involved in the degradation of collagen in resin-dentin interfaces. This study evaluated whether collagen degradation can be prevented by chlorhexidine digluconate (CHX) after different dentin demineralization procedures. The demineralization of human dentin was performed with phosphoric acid (PA), EDTA or acidic monomers (Clearfil SE Bond and Xeno V). Specimens were stored (for 24h, or for 1 or 3wk) in the presence or absence of CHX. In half of the groups, active MMP-2 was incorporated into the storage solution. At the end of each storage period, the C-terminal telopeptide (ICTP) concentration (which indicates the amount of collagen degradation) was measured in the storage solution. Collagen degradation was higher in PA- and EDTA-demineralized dentin. Chlorhexidine digluconate reduced collagen degradation in these groups only for 24h. When dentin was demineralized with Clearfil SE Bond or Xeno V, collagen degradation was reduced by up to 30%, but the addition of exogenous MMP-2 significantly increased collagen degradation. In self-etchant-treated dentin, the inhibitory effect of CHX on MMPs lasted for up to 3wk. Treating dentin with EDTA, PA or self-etching agents produces enough demineralization to permit cleavage of the exposed collagen. Monomer infiltration may exert protection on demineralized collagen, probably through immobilization of MMPs. The partial inhibitory action of CHX on MMP activity produced by self-etching adhesives was prolonged compared with the short-acting PA- or EDTA-treated dentin.

KW - Chlorhexidine

KW - Collagen degradation

KW - Dentin

KW - Matrix metalloproteinases

KW - Self-etching adhesives

UR - http://www.scopus.com/inward/record.url?scp=78751543211&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78751543211&partnerID=8YFLogxK

U2 - 10.1111/j.1600-0722.2010.00789.x

DO - 10.1111/j.1600-0722.2010.00789.x

M3 - Article

C2 - 21244516

AN - SCOPUS:78751543211

VL - 119

SP - 79

EP - 85

JO - European Journal of Oral Sciences

JF - European Journal of Oral Sciences

SN - 0909-8836

IS - 1

ER -