Effect of dimethyl sulfoxide on dentin collagen

P. Mehtälä, D. H. Pashley, L. Tjäderhane

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objectives Infiltration of adhesive on dentin matrix depends on interaction of surface and adhesive. Interaction depends on dentin wettability, which can be enhanced either by increasing dentin surface energy or lowering the surface energy of adhesive. The objective was to examine the effect of dimethyl sulfoxide (DMSO) on demineralized dentin wettability and dentin organic matrix expansion. Methods Acid-etched human dentin was used for sessile drop contact angle measurement to test surface wetting on 1–5% DMSO-treated demineralized dentin surface, and linear variable differential transformer (LVDT) to measure expansion/shrinkage of dentinal matrix. DMSO-water binary liquids were examined for surface tension changes through concentrations from 0 to 100% DMSO. Kruskal–Wallis and Mann–Whitney tests were used to test the differences in dentin wettability, expansion and shrinkage, and Spearman test to test the correlation between DMSO concentration and water surface tension. The level of significance was p < 0.05. Results Pretreatment with 1–5% DMSO caused statistically significant concentration-dependent increase in wetting: the immediate contact angles decreased by 11.8% and 46.6% and 60 s contact angles by 9.5% and 47.4% with 1% and 5% DMSO, respectively. DMSO-water mixtures concentration-dependently expanded demineralized dentin samples less than pure water, except with high (≥80%) DMSO concentrations which expanded demineralized dentin more than water. Drying times of LVDT samples increased significantly with the use of DMSO. Significance Increased dentin wettability may explain the previously demonstrated increase in adhesive penetration with DMSO-treated dentin, and together with the expansion of collagen matrix after drying may also explain previously observed increase in dentin adhesive bonding.

Original languageEnglish (US)
Pages (from-to)915-922
Number of pages8
JournalDental Materials
Volume33
Issue number8
DOIs
StatePublished - Aug 2017

Fingerprint

Dimethyl sulfoxide
Dentin
Dimethyl Sulfoxide
Collagen
Wetting
Wettability
Adhesives
Water
Contact angle
Surface Tension
Interfacial energy
Surface tension
Drying
Angle measurement
Infiltration

Keywords

  • Adhesion
  • Collagen expansion
  • Collagen shrinkage
  • Contact angle
  • DMSO
  • Wettability

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Mehtälä, P., Pashley, D. H., & Tjäderhane, L. (2017). Effect of dimethyl sulfoxide on dentin collagen. Dental Materials, 33(8), 915-922. https://doi.org/10.1016/j.dental.2017.04.018

Effect of dimethyl sulfoxide on dentin collagen. / Mehtälä, P.; Pashley, D. H.; Tjäderhane, L.

In: Dental Materials, Vol. 33, No. 8, 08.2017, p. 915-922.

Research output: Contribution to journalArticle

Mehtälä, P, Pashley, DH & Tjäderhane, L 2017, 'Effect of dimethyl sulfoxide on dentin collagen', Dental Materials, vol. 33, no. 8, pp. 915-922. https://doi.org/10.1016/j.dental.2017.04.018
Mehtälä, P. ; Pashley, D. H. ; Tjäderhane, L. / Effect of dimethyl sulfoxide on dentin collagen. In: Dental Materials. 2017 ; Vol. 33, No. 8. pp. 915-922.
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abstract = "Objectives Infiltration of adhesive on dentin matrix depends on interaction of surface and adhesive. Interaction depends on dentin wettability, which can be enhanced either by increasing dentin surface energy or lowering the surface energy of adhesive. The objective was to examine the effect of dimethyl sulfoxide (DMSO) on demineralized dentin wettability and dentin organic matrix expansion. Methods Acid-etched human dentin was used for sessile drop contact angle measurement to test surface wetting on 1–5{\%} DMSO-treated demineralized dentin surface, and linear variable differential transformer (LVDT) to measure expansion/shrinkage of dentinal matrix. DMSO-water binary liquids were examined for surface tension changes through concentrations from 0 to 100{\%} DMSO. Kruskal–Wallis and Mann–Whitney tests were used to test the differences in dentin wettability, expansion and shrinkage, and Spearman test to test the correlation between DMSO concentration and water surface tension. The level of significance was p < 0.05. Results Pretreatment with 1–5{\%} DMSO caused statistically significant concentration-dependent increase in wetting: the immediate contact angles decreased by 11.8{\%} and 46.6{\%} and 60 s contact angles by 9.5{\%} and 47.4{\%} with 1{\%} and 5{\%} DMSO, respectively. DMSO-water mixtures concentration-dependently expanded demineralized dentin samples less than pure water, except with high (≥80{\%}) DMSO concentrations which expanded demineralized dentin more than water. Drying times of LVDT samples increased significantly with the use of DMSO. Significance Increased dentin wettability may explain the previously demonstrated increase in adhesive penetration with DMSO-treated dentin, and together with the expansion of collagen matrix after drying may also explain previously observed increase in dentin adhesive bonding.",
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AB - Objectives Infiltration of adhesive on dentin matrix depends on interaction of surface and adhesive. Interaction depends on dentin wettability, which can be enhanced either by increasing dentin surface energy or lowering the surface energy of adhesive. The objective was to examine the effect of dimethyl sulfoxide (DMSO) on demineralized dentin wettability and dentin organic matrix expansion. Methods Acid-etched human dentin was used for sessile drop contact angle measurement to test surface wetting on 1–5% DMSO-treated demineralized dentin surface, and linear variable differential transformer (LVDT) to measure expansion/shrinkage of dentinal matrix. DMSO-water binary liquids were examined for surface tension changes through concentrations from 0 to 100% DMSO. Kruskal–Wallis and Mann–Whitney tests were used to test the differences in dentin wettability, expansion and shrinkage, and Spearman test to test the correlation between DMSO concentration and water surface tension. The level of significance was p < 0.05. Results Pretreatment with 1–5% DMSO caused statistically significant concentration-dependent increase in wetting: the immediate contact angles decreased by 11.8% and 46.6% and 60 s contact angles by 9.5% and 47.4% with 1% and 5% DMSO, respectively. DMSO-water mixtures concentration-dependently expanded demineralized dentin samples less than pure water, except with high (≥80%) DMSO concentrations which expanded demineralized dentin more than water. Drying times of LVDT samples increased significantly with the use of DMSO. Significance Increased dentin wettability may explain the previously demonstrated increase in adhesive penetration with DMSO-treated dentin, and together with the expansion of collagen matrix after drying may also explain previously observed increase in dentin adhesive bonding.

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KW - DMSO

KW - Wettability

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