Effect of heat shock on function of frozen/thawed bull spermatozoa

V. H. Monterroso, K. C. Drury, A. D. Ealy, J. L. Edwards, P. J. Hansen

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Deposition of spermatozoa in the reproductive tract of hyperthermic cows could conceivably result in sperm damage. Accordingly, a series of experiments tested the effects of heat shock on functional characteristics and free radical production of bull spermatozoa. Viability was reduced slightly by short-term (1 to 3 h) culture at 42 and 43 °C as compared with culture at 39 °C. There was no effect of culture at 42 °C on the ability of spermatozoa to undergo swim-up or of 42 °C on the percentage of motile spermatozoa. However, exposure to 41 °C for 3 h reduced percentage of motile sperm, 41 and 42 °C reduced sperm velocity and 43 °C decreased the proportion of spermatozoa undergoing swim-up. In other experiments, there was no effect of heat shock (41 or 42 °C for 1 to 3 h) on DNA integrity, presence of intact acrosomes, or fertilizing ability of the spermatozoa. Superoxide production by spermatozoa was higher at 42 °C than at 39 or 41 °C, but there was no detectable hydrogen peroxide production at any temperature. The antioxidant, glutathione, tended to improve the ability of spermatozoa to undergo swim-up at 39 °C but not at 43 °C. Taken together, these results suggest that heat shock of a magnitude similar to that seen in vivo (41 to 42 °C) has little effect on sperm functions that affect fertilizing capability.

Original languageEnglish (US)
Pages (from-to)947-961
Number of pages15
JournalTheriogenology
Volume44
Issue number7
DOIs
StatePublished - Jan 1 1995

Fingerprint

heat stress
Spermatozoa
Shock
bulls
Hot Temperature
spermatozoa
Acrosome
acrosome
Superoxides
superoxide anion
Hydrogen Peroxide
functional properties
Free Radicals
Glutathione
hydrogen peroxide
glutathione
Antioxidants
viability
antioxidants
cows

Keywords

  • bull
  • fertilization
  • free radical
  • heat shock
  • motility
  • spermatozoa

ASJC Scopus subject areas

  • Small Animals
  • Food Animals
  • Animal Science and Zoology
  • Equine

Cite this

Monterroso, V. H., Drury, K. C., Ealy, A. D., Edwards, J. L., & Hansen, P. J. (1995). Effect of heat shock on function of frozen/thawed bull spermatozoa. Theriogenology, 44(7), 947-961. https://doi.org/10.1016/0093-691X(95)00282-D

Effect of heat shock on function of frozen/thawed bull spermatozoa. / Monterroso, V. H.; Drury, K. C.; Ealy, A. D.; Edwards, J. L.; Hansen, P. J.

In: Theriogenology, Vol. 44, No. 7, 01.01.1995, p. 947-961.

Research output: Contribution to journalArticle

Monterroso, VH, Drury, KC, Ealy, AD, Edwards, JL & Hansen, PJ 1995, 'Effect of heat shock on function of frozen/thawed bull spermatozoa', Theriogenology, vol. 44, no. 7, pp. 947-961. https://doi.org/10.1016/0093-691X(95)00282-D
Monterroso VH, Drury KC, Ealy AD, Edwards JL, Hansen PJ. Effect of heat shock on function of frozen/thawed bull spermatozoa. Theriogenology. 1995 Jan 1;44(7):947-961. https://doi.org/10.1016/0093-691X(95)00282-D
Monterroso, V. H. ; Drury, K. C. ; Ealy, A. D. ; Edwards, J. L. ; Hansen, P. J. / Effect of heat shock on function of frozen/thawed bull spermatozoa. In: Theriogenology. 1995 ; Vol. 44, No. 7. pp. 947-961.
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