Effect of high K+ exposure on phosphoinositide metabolism in frog skeletal muscle

Zheng Dong, Ke Ying Chen, Pei Hong Zhu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Using [3H]myo-inositol labeled frog skeletal muscles, we have studied the effect of high K+ exposure on phosphoinositide metabolism. After 12 hours labeling, 80mM K+ exposure induced a time-dependent change. The labeling associated with phosphatidylinositol(PI) and phosphatidylinositol 4-phosphate(PIP) gradually increased and decreased, respectively. The labeled phosphatidylinositol 4,5-bisphosphate(PIP2) first decreased, and then recovered. An accumulation of the labeling in inositol phosphates was shown. In shortening the labeling to 30min, 15min high K+ exposure was found to only increase the labeling in all fractions. Taken together, these results show that high K+ exposure can activate the turnover of phosphoinositides, which is consistent with the hypothesis that the metabolism of phosphoiositides may regulate excitation-contraction(e-c) coupling.

Original languageEnglish (US)
Pages (from-to)767-772
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume182
Issue number2
DOIs
StatePublished - Jan 31 1992

Fingerprint

Phosphatidylinositols
Metabolism
Anura
Labeling
Muscle
Skeletal Muscle
Excitation Contraction Coupling
Inositol Phosphates
Inositol

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Effect of high K+ exposure on phosphoinositide metabolism in frog skeletal muscle. / Dong, Zheng; Chen, Ke Ying; Zhu, Pei Hong.

In: Biochemical and Biophysical Research Communications, Vol. 182, No. 2, 31.01.1992, p. 767-772.

Research output: Contribution to journalArticle

@article{aa54695ae6fb4b3687be73e91c93a391,
title = "Effect of high K+ exposure on phosphoinositide metabolism in frog skeletal muscle",
abstract = "Using [3H]myo-inositol labeled frog skeletal muscles, we have studied the effect of high K+ exposure on phosphoinositide metabolism. After 12 hours labeling, 80mM K+ exposure induced a time-dependent change. The labeling associated with phosphatidylinositol(PI) and phosphatidylinositol 4-phosphate(PIP) gradually increased and decreased, respectively. The labeled phosphatidylinositol 4,5-bisphosphate(PIP2) first decreased, and then recovered. An accumulation of the labeling in inositol phosphates was shown. In shortening the labeling to 30min, 15min high K+ exposure was found to only increase the labeling in all fractions. Taken together, these results show that high K+ exposure can activate the turnover of phosphoinositides, which is consistent with the hypothesis that the metabolism of phosphoiositides may regulate excitation-contraction(e-c) coupling.",
author = "Zheng Dong and Chen, {Ke Ying} and Zhu, {Pei Hong}",
year = "1992",
month = "1",
day = "31",
doi = "10.1016/0006-291X(92)91798-U",
language = "English (US)",
volume = "182",
pages = "767--772",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Effect of high K+ exposure on phosphoinositide metabolism in frog skeletal muscle

AU - Dong, Zheng

AU - Chen, Ke Ying

AU - Zhu, Pei Hong

PY - 1992/1/31

Y1 - 1992/1/31

N2 - Using [3H]myo-inositol labeled frog skeletal muscles, we have studied the effect of high K+ exposure on phosphoinositide metabolism. After 12 hours labeling, 80mM K+ exposure induced a time-dependent change. The labeling associated with phosphatidylinositol(PI) and phosphatidylinositol 4-phosphate(PIP) gradually increased and decreased, respectively. The labeled phosphatidylinositol 4,5-bisphosphate(PIP2) first decreased, and then recovered. An accumulation of the labeling in inositol phosphates was shown. In shortening the labeling to 30min, 15min high K+ exposure was found to only increase the labeling in all fractions. Taken together, these results show that high K+ exposure can activate the turnover of phosphoinositides, which is consistent with the hypothesis that the metabolism of phosphoiositides may regulate excitation-contraction(e-c) coupling.

AB - Using [3H]myo-inositol labeled frog skeletal muscles, we have studied the effect of high K+ exposure on phosphoinositide metabolism. After 12 hours labeling, 80mM K+ exposure induced a time-dependent change. The labeling associated with phosphatidylinositol(PI) and phosphatidylinositol 4-phosphate(PIP) gradually increased and decreased, respectively. The labeled phosphatidylinositol 4,5-bisphosphate(PIP2) first decreased, and then recovered. An accumulation of the labeling in inositol phosphates was shown. In shortening the labeling to 30min, 15min high K+ exposure was found to only increase the labeling in all fractions. Taken together, these results show that high K+ exposure can activate the turnover of phosphoinositides, which is consistent with the hypothesis that the metabolism of phosphoiositides may regulate excitation-contraction(e-c) coupling.

UR - http://www.scopus.com/inward/record.url?scp=0026575145&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026575145&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(92)91798-U

DO - 10.1016/0006-291X(92)91798-U

M3 - Article

C2 - 1310393

AN - SCOPUS:0026575145

VL - 182

SP - 767

EP - 772

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -