Effect of human stem cells labeled with ferumoxides-poly-L-lysine on hematologic and biochemical measurements in rats

Gene T. Yocum, Lindsey B. Wilson, Parwana Ashari, E. Kay Jordan, Joseph A. Frank, Ali S. Arbab

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

PURPOSE: To determine whether ferumoxides-poly-L-lysine (PLL) complex-labeled mesenchymal stem cells (MSCs) or ferumoxides-PLL complex alone alters hematologic, blood chemistry, renal function, and/or liver function measurements after being intravenously infused into rats. MATERIALS AND METHODS: Twenty-five rats (group 1) received intravenous injections of labeled MSCs, and 25 additional rats (group 2) received intravenous injections of ferumoxides-PLL complex only. Complete blood counts, liver and renal function test results, and serum electrolyte and iron concentrations were measured for 42 days after the injections and compared with those measured in five control rats (group 3). To determine the duration of labeled MSCs in the circulation, venous blood was serially drawn from five additional rats (group 4) that were injected with labeled MSCs. Analyses of variance (ANOVA) followed by Fisher protected least significant difference post hoc tests were used to statistically analyze results. P < .05 was considered to indicate significance in all analyses. RESULTS: Administration of neither labeled MSCs nor ferumoxides-PLL complex had a significant effect on hematologic or blood chemistry indicators of organ function. Of the parameters measured, only hemoglobin concentration and mean corpuscular volume (MCV) in the rats injected with labeled MSCs, as well as MCV and hemoglobin, alkaline phosphatase, aspartate aminotransferase, and direct bilirubin concentrations in the rats injected with ferumoxides-PLL complex, varied significantly during the 42-day postinjection period (P < .05, ANOVA). No other measurements, including serum electrolyte and iron concentrations, changed significantly during the test period (P > .05). Furthermore, injected labeled MSCs had cleared from the peripheral circulation by 15 minutes after injection. CONCLUSION: Results indicate that infusing cells that are magnetically labeled with ferumoxides-PLL complex into rats does not alter biochemical or hematologic measures of organ function in a clinically relevant or preclusive manner.

Original languageEnglish (US)
Pages (from-to)547-552
Number of pages6
JournalRadiology
Volume235
Issue number2
DOIs
StatePublished - May 1 2005

Fingerprint

Lysine
Mesenchymal Stromal Cells
Stem Cells
Intravenous Injections
Kidney
Injections
Blood Cell Count
Blood Circulation
Liver Function Tests
Electrolytes
ferumoxides
Analysis of Variance
Iron
Control Groups
Liver
Serum

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Effect of human stem cells labeled with ferumoxides-poly-L-lysine on hematologic and biochemical measurements in rats. / Yocum, Gene T.; Wilson, Lindsey B.; Ashari, Parwana; Jordan, E. Kay; Frank, Joseph A.; Arbab, Ali S.

In: Radiology, Vol. 235, No. 2, 01.05.2005, p. 547-552.

Research output: Contribution to journalArticle

Yocum, Gene T. ; Wilson, Lindsey B. ; Ashari, Parwana ; Jordan, E. Kay ; Frank, Joseph A. ; Arbab, Ali S. / Effect of human stem cells labeled with ferumoxides-poly-L-lysine on hematologic and biochemical measurements in rats. In: Radiology. 2005 ; Vol. 235, No. 2. pp. 547-552.
@article{e67998a01b524804913ca31a1b43e840,
title = "Effect of human stem cells labeled with ferumoxides-poly-L-lysine on hematologic and biochemical measurements in rats",
abstract = "PURPOSE: To determine whether ferumoxides-poly-L-lysine (PLL) complex-labeled mesenchymal stem cells (MSCs) or ferumoxides-PLL complex alone alters hematologic, blood chemistry, renal function, and/or liver function measurements after being intravenously infused into rats. MATERIALS AND METHODS: Twenty-five rats (group 1) received intravenous injections of labeled MSCs, and 25 additional rats (group 2) received intravenous injections of ferumoxides-PLL complex only. Complete blood counts, liver and renal function test results, and serum electrolyte and iron concentrations were measured for 42 days after the injections and compared with those measured in five control rats (group 3). To determine the duration of labeled MSCs in the circulation, venous blood was serially drawn from five additional rats (group 4) that were injected with labeled MSCs. Analyses of variance (ANOVA) followed by Fisher protected least significant difference post hoc tests were used to statistically analyze results. P < .05 was considered to indicate significance in all analyses. RESULTS: Administration of neither labeled MSCs nor ferumoxides-PLL complex had a significant effect on hematologic or blood chemistry indicators of organ function. Of the parameters measured, only hemoglobin concentration and mean corpuscular volume (MCV) in the rats injected with labeled MSCs, as well as MCV and hemoglobin, alkaline phosphatase, aspartate aminotransferase, and direct bilirubin concentrations in the rats injected with ferumoxides-PLL complex, varied significantly during the 42-day postinjection period (P < .05, ANOVA). No other measurements, including serum electrolyte and iron concentrations, changed significantly during the test period (P > .05). Furthermore, injected labeled MSCs had cleared from the peripheral circulation by 15 minutes after injection. CONCLUSION: Results indicate that infusing cells that are magnetically labeled with ferumoxides-PLL complex into rats does not alter biochemical or hematologic measures of organ function in a clinically relevant or preclusive manner.",
author = "Yocum, {Gene T.} and Wilson, {Lindsey B.} and Parwana Ashari and Jordan, {E. Kay} and Frank, {Joseph A.} and Arbab, {Ali S.}",
year = "2005",
month = "5",
day = "1",
doi = "10.1148/radiol.2352040383",
language = "English (US)",
volume = "235",
pages = "547--552",
journal = "Radiology",
issn = "0033-8419",
publisher = "Radiological Society of North America Inc.",
number = "2",

}

TY - JOUR

T1 - Effect of human stem cells labeled with ferumoxides-poly-L-lysine on hematologic and biochemical measurements in rats

AU - Yocum, Gene T.

AU - Wilson, Lindsey B.

AU - Ashari, Parwana

AU - Jordan, E. Kay

AU - Frank, Joseph A.

AU - Arbab, Ali S.

PY - 2005/5/1

Y1 - 2005/5/1

N2 - PURPOSE: To determine whether ferumoxides-poly-L-lysine (PLL) complex-labeled mesenchymal stem cells (MSCs) or ferumoxides-PLL complex alone alters hematologic, blood chemistry, renal function, and/or liver function measurements after being intravenously infused into rats. MATERIALS AND METHODS: Twenty-five rats (group 1) received intravenous injections of labeled MSCs, and 25 additional rats (group 2) received intravenous injections of ferumoxides-PLL complex only. Complete blood counts, liver and renal function test results, and serum electrolyte and iron concentrations were measured for 42 days after the injections and compared with those measured in five control rats (group 3). To determine the duration of labeled MSCs in the circulation, venous blood was serially drawn from five additional rats (group 4) that were injected with labeled MSCs. Analyses of variance (ANOVA) followed by Fisher protected least significant difference post hoc tests were used to statistically analyze results. P < .05 was considered to indicate significance in all analyses. RESULTS: Administration of neither labeled MSCs nor ferumoxides-PLL complex had a significant effect on hematologic or blood chemistry indicators of organ function. Of the parameters measured, only hemoglobin concentration and mean corpuscular volume (MCV) in the rats injected with labeled MSCs, as well as MCV and hemoglobin, alkaline phosphatase, aspartate aminotransferase, and direct bilirubin concentrations in the rats injected with ferumoxides-PLL complex, varied significantly during the 42-day postinjection period (P < .05, ANOVA). No other measurements, including serum electrolyte and iron concentrations, changed significantly during the test period (P > .05). Furthermore, injected labeled MSCs had cleared from the peripheral circulation by 15 minutes after injection. CONCLUSION: Results indicate that infusing cells that are magnetically labeled with ferumoxides-PLL complex into rats does not alter biochemical or hematologic measures of organ function in a clinically relevant or preclusive manner.

AB - PURPOSE: To determine whether ferumoxides-poly-L-lysine (PLL) complex-labeled mesenchymal stem cells (MSCs) or ferumoxides-PLL complex alone alters hematologic, blood chemistry, renal function, and/or liver function measurements after being intravenously infused into rats. MATERIALS AND METHODS: Twenty-five rats (group 1) received intravenous injections of labeled MSCs, and 25 additional rats (group 2) received intravenous injections of ferumoxides-PLL complex only. Complete blood counts, liver and renal function test results, and serum electrolyte and iron concentrations were measured for 42 days after the injections and compared with those measured in five control rats (group 3). To determine the duration of labeled MSCs in the circulation, venous blood was serially drawn from five additional rats (group 4) that were injected with labeled MSCs. Analyses of variance (ANOVA) followed by Fisher protected least significant difference post hoc tests were used to statistically analyze results. P < .05 was considered to indicate significance in all analyses. RESULTS: Administration of neither labeled MSCs nor ferumoxides-PLL complex had a significant effect on hematologic or blood chemistry indicators of organ function. Of the parameters measured, only hemoglobin concentration and mean corpuscular volume (MCV) in the rats injected with labeled MSCs, as well as MCV and hemoglobin, alkaline phosphatase, aspartate aminotransferase, and direct bilirubin concentrations in the rats injected with ferumoxides-PLL complex, varied significantly during the 42-day postinjection period (P < .05, ANOVA). No other measurements, including serum electrolyte and iron concentrations, changed significantly during the test period (P > .05). Furthermore, injected labeled MSCs had cleared from the peripheral circulation by 15 minutes after injection. CONCLUSION: Results indicate that infusing cells that are magnetically labeled with ferumoxides-PLL complex into rats does not alter biochemical or hematologic measures of organ function in a clinically relevant or preclusive manner.

UR - http://www.scopus.com/inward/record.url?scp=17644376566&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=17644376566&partnerID=8YFLogxK

U2 - 10.1148/radiol.2352040383

DO - 10.1148/radiol.2352040383

M3 - Article

C2 - 15858093

AN - SCOPUS:17644376566

VL - 235

SP - 547

EP - 552

JO - Radiology

JF - Radiology

SN - 0033-8419

IS - 2

ER -