Effect of hypoxia on angiotensin-converting enzyme activity and gene expression of intrapulmonary artery smooth muscle cells

Tian Zheng Yu, Chuan Tao Ma

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In order to observe the effect of hypoxia on pulmonary artery smooth muscle cells (PASMC), angiotensin-converting enzyme (ACE) activity was measured by colometry and the expression of ACE mRNA was detected by Northern blot, using rabbit isolated and cultured PASMC. The main results are as follows: (1) under normoxia both ACE activity and gene expression could be detected in PASMC; and (2) the expression level of ACE mRNA, after an initial latent period of 6 h hypoxia, began to show a marked increase with time in the next 12, 24 and 48 h. Exposure to hypoxia for 24 h resulted in an increase in either culture medium or cell ACE activity, as compared with that under normoxia. The results suggest that PASMC ACE increased by hypoxia might play an important role in the development of hypoxic pulmonary hypertension.

Original languageEnglish (US)
Pages (from-to)623-628
Number of pages6
JournalActa Physiologica Sinica
Volume50
Issue number6
StatePublished - Dec 1 1998

Fingerprint

Peptidyl-Dipeptidase A
Smooth Muscle Myocytes
Arteries
Gene Expression
Pulmonary Artery
Messenger RNA
Pulmonary Hypertension
Northern Blotting
Culture Media
Hypoxia
Rabbits

Keywords

  • Angiotension converting enzyme (ACE)
  • Hypoxia
  • Pulmonary artery
  • Rabbit
  • Vascular smooth muscle cell

ASJC Scopus subject areas

  • Physiology

Cite this

Effect of hypoxia on angiotensin-converting enzyme activity and gene expression of intrapulmonary artery smooth muscle cells. / Yu, Tian Zheng; Ma, Chuan Tao.

In: Acta Physiologica Sinica, Vol. 50, No. 6, 01.12.1998, p. 623-628.

Research output: Contribution to journalArticle

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