Effect of hypoxia on angiotensin-converting enzyme activity and gene expression of intrapulmonary artery smooth muscle cells

Tian Zheng Yu, Chuan Tao Ma

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

In order to observe the effect of hypoxia on pulmonary artery smooth muscle cells (PASMC), angiotensin-converting enzyme (ACE) activity was measured by colometry and the expression of ACE mRNA was detected by Northern blot, using rabbit isolated and cultured PASMC. The main results are as follows: (1) under normoxia both ACE activity and gene expression could be detected in PASMC; and (2) the expression level of ACE mRNA, after an initial latent period of 6 h hypoxia, began to show a marked increase with time in the next 12, 24 and 48 h. Exposure to hypoxia for 24 h resulted in an increase in either culture medium or cell ACE activity, as compared with that under normoxia. The results suggest that PASMC ACE increased by hypoxia might play an important role in the development of hypoxic pulmonary hypertension.

Original languageEnglish (US)
Pages (from-to)623-628
Number of pages6
JournalActa Physiologica Sinica
Volume50
Issue number6
StatePublished - Dec 1 1998

Keywords

  • Angiotension converting enzyme (ACE)
  • Hypoxia
  • Pulmonary artery
  • Rabbit
  • Vascular smooth muscle cell

ASJC Scopus subject areas

  • Physiology

Fingerprint Dive into the research topics of 'Effect of hypoxia on angiotensin-converting enzyme activity and gene expression of intrapulmonary artery smooth muscle cells'. Together they form a unique fingerprint.

  • Cite this