Effect of irradiation on thermal sensitivity of bone marrow progenitors

Nahid F Mivechi, Gloria C. Li

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The heat sensitivity of murine CFU-GM and CFU-E following 2.5 Gy of total body irradiation (TBI) was studied. C3H f/Sed female mice were treated with 2.5 Gy TBI and femoral bone marrow was heated in vitro at 43°C. CFU-GM show heat radio-sensitization when bone marrow was heated immediately following irradiation. There was a brief decline in heat and radiation interaction when cells were heated 3 hours following 2.5 Gy of TBI, but heat radiosensitization returned to its maximum from 1 to 2 days following irradiation and remained significantly different from the control on days 5 and 7 following irradiation. The heat and radiation interaction disappeared by 30 days. CFU-E shows significant heat radiosensitization only on day 2 following 2.5 Gy of TBI. Total nucleated cells per femur showed a decrease by 70 per cent in days 1 to 2 following TBI, recovered to control values by day 5, and did not correlate with the changes in heat radiosensitization. Cell cycle analysis of CFU-GM using hydroxyurea showed no significant changes in cell cycle parameters on days 1 and 2 following 2.5 Gy, when maximum heat sensitization was observed. It is concluded that bone marrow progenitors may respond in a different way from other normal tissues to heat and irradiation sequencing, and that these differences must be considered when designing clinical trials.

Original languageEnglish (US)
Pages (from-to)537-546
Number of pages10
JournalInternational Journal of Hyperthermia
Volume4
Issue number5
DOIs
StatePublished - Jan 1 1988
Externally publishedYes

Fingerprint

Hot Temperature
Bone Marrow
Whole-Body Irradiation
Granulocyte-Macrophage Progenitor Cells
Erythroid Precursor Cells
Cell Cycle
Radiation
Hydroxyurea
Thigh
Radio
Cell Communication
Femur
Clinical Trials

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Cancer Research

Cite this

Effect of irradiation on thermal sensitivity of bone marrow progenitors. / Mivechi, Nahid F; Li, Gloria C.

In: International Journal of Hyperthermia, Vol. 4, No. 5, 01.01.1988, p. 537-546.

Research output: Contribution to journalArticle

@article{8206cae735b84194a501cf35c25413b7,
title = "Effect of irradiation on thermal sensitivity of bone marrow progenitors",
abstract = "The heat sensitivity of murine CFU-GM and CFU-E following 2.5 Gy of total body irradiation (TBI) was studied. C3H f/Sed female mice were treated with 2.5 Gy TBI and femoral bone marrow was heated in vitro at 43°C. CFU-GM show heat radio-sensitization when bone marrow was heated immediately following irradiation. There was a brief decline in heat and radiation interaction when cells were heated 3 hours following 2.5 Gy of TBI, but heat radiosensitization returned to its maximum from 1 to 2 days following irradiation and remained significantly different from the control on days 5 and 7 following irradiation. The heat and radiation interaction disappeared by 30 days. CFU-E shows significant heat radiosensitization only on day 2 following 2.5 Gy of TBI. Total nucleated cells per femur showed a decrease by 70 per cent in days 1 to 2 following TBI, recovered to control values by day 5, and did not correlate with the changes in heat radiosensitization. Cell cycle analysis of CFU-GM using hydroxyurea showed no significant changes in cell cycle parameters on days 1 and 2 following 2.5 Gy, when maximum heat sensitization was observed. It is concluded that bone marrow progenitors may respond in a different way from other normal tissues to heat and irradiation sequencing, and that these differences must be considered when designing clinical trials.",
author = "Mivechi, {Nahid F} and Li, {Gloria C.}",
year = "1988",
month = "1",
day = "1",
doi = "10.3109/02656738809027697",
language = "English (US)",
volume = "4",
pages = "537--546",
journal = "International Journal of Hyperthermia",
issn = "0265-6736",
publisher = "Informa Healthcare",
number = "5",

}

TY - JOUR

T1 - Effect of irradiation on thermal sensitivity of bone marrow progenitors

AU - Mivechi, Nahid F

AU - Li, Gloria C.

PY - 1988/1/1

Y1 - 1988/1/1

N2 - The heat sensitivity of murine CFU-GM and CFU-E following 2.5 Gy of total body irradiation (TBI) was studied. C3H f/Sed female mice were treated with 2.5 Gy TBI and femoral bone marrow was heated in vitro at 43°C. CFU-GM show heat radio-sensitization when bone marrow was heated immediately following irradiation. There was a brief decline in heat and radiation interaction when cells were heated 3 hours following 2.5 Gy of TBI, but heat radiosensitization returned to its maximum from 1 to 2 days following irradiation and remained significantly different from the control on days 5 and 7 following irradiation. The heat and radiation interaction disappeared by 30 days. CFU-E shows significant heat radiosensitization only on day 2 following 2.5 Gy of TBI. Total nucleated cells per femur showed a decrease by 70 per cent in days 1 to 2 following TBI, recovered to control values by day 5, and did not correlate with the changes in heat radiosensitization. Cell cycle analysis of CFU-GM using hydroxyurea showed no significant changes in cell cycle parameters on days 1 and 2 following 2.5 Gy, when maximum heat sensitization was observed. It is concluded that bone marrow progenitors may respond in a different way from other normal tissues to heat and irradiation sequencing, and that these differences must be considered when designing clinical trials.

AB - The heat sensitivity of murine CFU-GM and CFU-E following 2.5 Gy of total body irradiation (TBI) was studied. C3H f/Sed female mice were treated with 2.5 Gy TBI and femoral bone marrow was heated in vitro at 43°C. CFU-GM show heat radio-sensitization when bone marrow was heated immediately following irradiation. There was a brief decline in heat and radiation interaction when cells were heated 3 hours following 2.5 Gy of TBI, but heat radiosensitization returned to its maximum from 1 to 2 days following irradiation and remained significantly different from the control on days 5 and 7 following irradiation. The heat and radiation interaction disappeared by 30 days. CFU-E shows significant heat radiosensitization only on day 2 following 2.5 Gy of TBI. Total nucleated cells per femur showed a decrease by 70 per cent in days 1 to 2 following TBI, recovered to control values by day 5, and did not correlate with the changes in heat radiosensitization. Cell cycle analysis of CFU-GM using hydroxyurea showed no significant changes in cell cycle parameters on days 1 and 2 following 2.5 Gy, when maximum heat sensitization was observed. It is concluded that bone marrow progenitors may respond in a different way from other normal tissues to heat and irradiation sequencing, and that these differences must be considered when designing clinical trials.

UR - http://www.scopus.com/inward/record.url?scp=0023892015&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023892015&partnerID=8YFLogxK

U2 - 10.3109/02656738809027697

DO - 10.3109/02656738809027697

M3 - Article

C2 - 3292668

AN - SCOPUS:0023892015

VL - 4

SP - 537

EP - 546

JO - International Journal of Hyperthermia

JF - International Journal of Hyperthermia

SN - 0265-6736

IS - 5

ER -