Effect of lead and cadmium co-exposure on testicular steroid metabolism and antioxidant system of adult male rats

Chirayukumar D Pandya, P. Pillai, L. P. Nampoothiri, N. Bhatt, S. Gupta, S. Gupta

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The mechanism of testicular toxicity of lead (Pb) and cadmium (Cd) is poorly understood. Previous studies focused on single metal-related changes in testicular toxicity. This study points towards the possible involvement of Pb- and Cd-induced oxidative stress in the suppression of steroidogenesis. The oxidative status of testis of adult male rats exposed to Pb acetate and cadmium acetate either alone or in combination at a dose of 0.025mgkg -1 body weight of metal intraperitoneally for 15days was studied. Pb and Cd caused an increase in reactive oxygen species (ROS) by elevating testicular malondialdehydes (MDA) and decrease in activities of testicular antioxidant enzymes superoxide dismutase (SOD), catalase, glucose 6 phosphate dehydrogenase (G6PDH) and glutathione-S-transferase (GST) in mitochondrial and/or post-mitochondrial fraction. Activities of steroidogenic enzymes 3β and 17β-hydroxysteroid dehydrogenase also decreased significantly leading to altered testosterone production. Metal-exposed groups showed significantly decreased testicular and epididymal sperm count. Epididymal sperm motility and viability was also decreased on Pb and Cd exposure. Cd exposure showed more toxic effect than lead exposure, while combined exposure demonstrated least toxicity. In vitro experiments showed that vitamin C restores steroidogenic enzyme activities, suggesting that Pb- and Cd-induced ROS inhibits the testicular steroidogenesis.

Original languageEnglish (US)
Pages (from-to)813-822
Number of pages10
JournalAndrologia
Volume44
Issue numberSUPPL.1
DOIs
StatePublished - May 1 2012

Fingerprint

Cadmium
Antioxidants
Steroids
Metals
Reactive Oxygen Species
Enzymes
Sperm Count
Sperm Motility
Glucosephosphate Dehydrogenase
Poisons
Glutathione Transferase
Malondialdehyde
Catalase
Ascorbic Acid
Superoxide Dismutase
Testosterone
Lead
Testis
Acetates
Oxidative Stress

Keywords

  • Antioxidant enzyme
  • Cadmium
  • Lead
  • Reactive oxygen species
  • Testis

ASJC Scopus subject areas

  • Endocrinology
  • Urology

Cite this

Pandya, C. D., Pillai, P., Nampoothiri, L. P., Bhatt, N., Gupta, S., & Gupta, S. (2012). Effect of lead and cadmium co-exposure on testicular steroid metabolism and antioxidant system of adult male rats. Andrologia, 44(SUPPL.1), 813-822. https://doi.org/10.1111/j.1439-0272.2010.01137.x

Effect of lead and cadmium co-exposure on testicular steroid metabolism and antioxidant system of adult male rats. / Pandya, Chirayukumar D; Pillai, P.; Nampoothiri, L. P.; Bhatt, N.; Gupta, S.; Gupta, S.

In: Andrologia, Vol. 44, No. SUPPL.1, 01.05.2012, p. 813-822.

Research output: Contribution to journalArticle

Pandya, CD, Pillai, P, Nampoothiri, LP, Bhatt, N, Gupta, S & Gupta, S 2012, 'Effect of lead and cadmium co-exposure on testicular steroid metabolism and antioxidant system of adult male rats', Andrologia, vol. 44, no. SUPPL.1, pp. 813-822. https://doi.org/10.1111/j.1439-0272.2010.01137.x
Pandya, Chirayukumar D ; Pillai, P. ; Nampoothiri, L. P. ; Bhatt, N. ; Gupta, S. ; Gupta, S. / Effect of lead and cadmium co-exposure on testicular steroid metabolism and antioxidant system of adult male rats. In: Andrologia. 2012 ; Vol. 44, No. SUPPL.1. pp. 813-822.
@article{71301bb6e7214ddeaa2da1a6a81e9100,
title = "Effect of lead and cadmium co-exposure on testicular steroid metabolism and antioxidant system of adult male rats",
abstract = "The mechanism of testicular toxicity of lead (Pb) and cadmium (Cd) is poorly understood. Previous studies focused on single metal-related changes in testicular toxicity. This study points towards the possible involvement of Pb- and Cd-induced oxidative stress in the suppression of steroidogenesis. The oxidative status of testis of adult male rats exposed to Pb acetate and cadmium acetate either alone or in combination at a dose of 0.025mgkg -1 body weight of metal intraperitoneally for 15days was studied. Pb and Cd caused an increase in reactive oxygen species (ROS) by elevating testicular malondialdehydes (MDA) and decrease in activities of testicular antioxidant enzymes superoxide dismutase (SOD), catalase, glucose 6 phosphate dehydrogenase (G6PDH) and glutathione-S-transferase (GST) in mitochondrial and/or post-mitochondrial fraction. Activities of steroidogenic enzymes 3β and 17β-hydroxysteroid dehydrogenase also decreased significantly leading to altered testosterone production. Metal-exposed groups showed significantly decreased testicular and epididymal sperm count. Epididymal sperm motility and viability was also decreased on Pb and Cd exposure. Cd exposure showed more toxic effect than lead exposure, while combined exposure demonstrated least toxicity. In vitro experiments showed that vitamin C restores steroidogenic enzyme activities, suggesting that Pb- and Cd-induced ROS inhibits the testicular steroidogenesis.",
keywords = "Antioxidant enzyme, Cadmium, Lead, Reactive oxygen species, Testis",
author = "Pandya, {Chirayukumar D} and P. Pillai and Nampoothiri, {L. P.} and N. Bhatt and S. Gupta and S. Gupta",
year = "2012",
month = "5",
day = "1",
doi = "10.1111/j.1439-0272.2010.01137.x",
language = "English (US)",
volume = "44",
pages = "813--822",
journal = "Andrologia",
issn = "0303-4569",
publisher = "Wiley-Blackwell",
number = "SUPPL.1",

}

TY - JOUR

T1 - Effect of lead and cadmium co-exposure on testicular steroid metabolism and antioxidant system of adult male rats

AU - Pandya, Chirayukumar D

AU - Pillai, P.

AU - Nampoothiri, L. P.

AU - Bhatt, N.

AU - Gupta, S.

AU - Gupta, S.

PY - 2012/5/1

Y1 - 2012/5/1

N2 - The mechanism of testicular toxicity of lead (Pb) and cadmium (Cd) is poorly understood. Previous studies focused on single metal-related changes in testicular toxicity. This study points towards the possible involvement of Pb- and Cd-induced oxidative stress in the suppression of steroidogenesis. The oxidative status of testis of adult male rats exposed to Pb acetate and cadmium acetate either alone or in combination at a dose of 0.025mgkg -1 body weight of metal intraperitoneally for 15days was studied. Pb and Cd caused an increase in reactive oxygen species (ROS) by elevating testicular malondialdehydes (MDA) and decrease in activities of testicular antioxidant enzymes superoxide dismutase (SOD), catalase, glucose 6 phosphate dehydrogenase (G6PDH) and glutathione-S-transferase (GST) in mitochondrial and/or post-mitochondrial fraction. Activities of steroidogenic enzymes 3β and 17β-hydroxysteroid dehydrogenase also decreased significantly leading to altered testosterone production. Metal-exposed groups showed significantly decreased testicular and epididymal sperm count. Epididymal sperm motility and viability was also decreased on Pb and Cd exposure. Cd exposure showed more toxic effect than lead exposure, while combined exposure demonstrated least toxicity. In vitro experiments showed that vitamin C restores steroidogenic enzyme activities, suggesting that Pb- and Cd-induced ROS inhibits the testicular steroidogenesis.

AB - The mechanism of testicular toxicity of lead (Pb) and cadmium (Cd) is poorly understood. Previous studies focused on single metal-related changes in testicular toxicity. This study points towards the possible involvement of Pb- and Cd-induced oxidative stress in the suppression of steroidogenesis. The oxidative status of testis of adult male rats exposed to Pb acetate and cadmium acetate either alone or in combination at a dose of 0.025mgkg -1 body weight of metal intraperitoneally for 15days was studied. Pb and Cd caused an increase in reactive oxygen species (ROS) by elevating testicular malondialdehydes (MDA) and decrease in activities of testicular antioxidant enzymes superoxide dismutase (SOD), catalase, glucose 6 phosphate dehydrogenase (G6PDH) and glutathione-S-transferase (GST) in mitochondrial and/or post-mitochondrial fraction. Activities of steroidogenic enzymes 3β and 17β-hydroxysteroid dehydrogenase also decreased significantly leading to altered testosterone production. Metal-exposed groups showed significantly decreased testicular and epididymal sperm count. Epididymal sperm motility and viability was also decreased on Pb and Cd exposure. Cd exposure showed more toxic effect than lead exposure, while combined exposure demonstrated least toxicity. In vitro experiments showed that vitamin C restores steroidogenic enzyme activities, suggesting that Pb- and Cd-induced ROS inhibits the testicular steroidogenesis.

KW - Antioxidant enzyme

KW - Cadmium

KW - Lead

KW - Reactive oxygen species

KW - Testis

UR - http://www.scopus.com/inward/record.url?scp=84861492297&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84861492297&partnerID=8YFLogxK

U2 - 10.1111/j.1439-0272.2010.01137.x

DO - 10.1111/j.1439-0272.2010.01137.x

M3 - Article

VL - 44

SP - 813

EP - 822

JO - Andrologia

JF - Andrologia

SN - 0303-4569

IS - SUPPL.1

ER -