Effect of lipopolysaccharide contamination on the attachment of osteoblast-like cells to titanium and titanium alloy in vitro.

R. A. Nouneh, J. C. Wataha, Philip Jerry Hanes, P. E. Lockwood

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Failing implants with loss of alveolar bone are associated with gram-negative bacteria that carry lipopolysaccharide (LPS) in the bacterial cell wall. Bony regeneration around these implants is still an unpredictable procedure due to the many clinical factors involved. One important factor is the presence of contaminants such as LPS on the implant surface. The effect of implant-associated LPS on the attachment of bone cells to the implant surface is unknown. This project investigated the effect of LPS on the attachment of osteoblast-like cells (MC3T3-E1) to titanium and titanium alloy surfaces in vitro. We hypothesized that LPS would inhibit bone cell attachment either through loss of cellular attachment sites or alteration of cellular function. Three experimental approaches were used. First, alloy surfaces were exposed to LPS (100 microgram/mL) before the cells were allowed to attach. In the second approach, the cells were exposed to the LPS before they were allowed to attach. Last, the cells were allowed to attach before exposure to LPS. Cellular attachment to implant materials was measured by using a histochemical stain (MTT). The results indicated that LPS presence did not significantly (P > .05) alter osteoblast attachment to titanium or titanium alloy surfaces whether the exposure occurred before or after cellular adherence. It was concluded that LPS did not directly effect the attachment of the MC3T3-E1 osteoblasts to these implant surfaces in vitro. Further research is needed to define the clinical liabilities of LPS during implant placement and maintenance.

Original languageEnglish (US)
Pages (from-to)174-179
Number of pages6
JournalThe Journal of oral implantology
Volume27
Issue number4
DOIs
StatePublished - Jan 1 2001

Fingerprint

Titanium
Osteoblasts
Lipopolysaccharides
In Vitro Techniques
Alveolar Bone Loss
Bone and Bones
Gram-Negative Bacteria
Cell Wall
Regeneration
Coloring Agents
Maintenance

ASJC Scopus subject areas

  • Oral Surgery

Cite this

Effect of lipopolysaccharide contamination on the attachment of osteoblast-like cells to titanium and titanium alloy in vitro. / Nouneh, R. A.; Wataha, J. C.; Hanes, Philip Jerry; Lockwood, P. E.

In: The Journal of oral implantology, Vol. 27, No. 4, 01.01.2001, p. 174-179.

Research output: Contribution to journalArticle

@article{eb53da3ba113413e97f32b13ed7d36b6,
title = "Effect of lipopolysaccharide contamination on the attachment of osteoblast-like cells to titanium and titanium alloy in vitro.",
abstract = "Failing implants with loss of alveolar bone are associated with gram-negative bacteria that carry lipopolysaccharide (LPS) in the bacterial cell wall. Bony regeneration around these implants is still an unpredictable procedure due to the many clinical factors involved. One important factor is the presence of contaminants such as LPS on the implant surface. The effect of implant-associated LPS on the attachment of bone cells to the implant surface is unknown. This project investigated the effect of LPS on the attachment of osteoblast-like cells (MC3T3-E1) to titanium and titanium alloy surfaces in vitro. We hypothesized that LPS would inhibit bone cell attachment either through loss of cellular attachment sites or alteration of cellular function. Three experimental approaches were used. First, alloy surfaces were exposed to LPS (100 microgram/mL) before the cells were allowed to attach. In the second approach, the cells were exposed to the LPS before they were allowed to attach. Last, the cells were allowed to attach before exposure to LPS. Cellular attachment to implant materials was measured by using a histochemical stain (MTT). The results indicated that LPS presence did not significantly (P > .05) alter osteoblast attachment to titanium or titanium alloy surfaces whether the exposure occurred before or after cellular adherence. It was concluded that LPS did not directly effect the attachment of the MC3T3-E1 osteoblasts to these implant surfaces in vitro. Further research is needed to define the clinical liabilities of LPS during implant placement and maintenance.",
author = "Nouneh, {R. A.} and Wataha, {J. C.} and Hanes, {Philip Jerry} and Lockwood, {P. E.}",
year = "2001",
month = "1",
day = "1",
doi = "10.1563/1548-1336(2001)027<0174:EOLCOT>2.3.CO;2",
language = "English (US)",
volume = "27",
pages = "174--179",
journal = "Journal of Oral Implantology",
issn = "0160-6972",
publisher = "Allen Press Inc.",
number = "4",

}

TY - JOUR

T1 - Effect of lipopolysaccharide contamination on the attachment of osteoblast-like cells to titanium and titanium alloy in vitro.

AU - Nouneh, R. A.

AU - Wataha, J. C.

AU - Hanes, Philip Jerry

AU - Lockwood, P. E.

PY - 2001/1/1

Y1 - 2001/1/1

N2 - Failing implants with loss of alveolar bone are associated with gram-negative bacteria that carry lipopolysaccharide (LPS) in the bacterial cell wall. Bony regeneration around these implants is still an unpredictable procedure due to the many clinical factors involved. One important factor is the presence of contaminants such as LPS on the implant surface. The effect of implant-associated LPS on the attachment of bone cells to the implant surface is unknown. This project investigated the effect of LPS on the attachment of osteoblast-like cells (MC3T3-E1) to titanium and titanium alloy surfaces in vitro. We hypothesized that LPS would inhibit bone cell attachment either through loss of cellular attachment sites or alteration of cellular function. Three experimental approaches were used. First, alloy surfaces were exposed to LPS (100 microgram/mL) before the cells were allowed to attach. In the second approach, the cells were exposed to the LPS before they were allowed to attach. Last, the cells were allowed to attach before exposure to LPS. Cellular attachment to implant materials was measured by using a histochemical stain (MTT). The results indicated that LPS presence did not significantly (P > .05) alter osteoblast attachment to titanium or titanium alloy surfaces whether the exposure occurred before or after cellular adherence. It was concluded that LPS did not directly effect the attachment of the MC3T3-E1 osteoblasts to these implant surfaces in vitro. Further research is needed to define the clinical liabilities of LPS during implant placement and maintenance.

AB - Failing implants with loss of alveolar bone are associated with gram-negative bacteria that carry lipopolysaccharide (LPS) in the bacterial cell wall. Bony regeneration around these implants is still an unpredictable procedure due to the many clinical factors involved. One important factor is the presence of contaminants such as LPS on the implant surface. The effect of implant-associated LPS on the attachment of bone cells to the implant surface is unknown. This project investigated the effect of LPS on the attachment of osteoblast-like cells (MC3T3-E1) to titanium and titanium alloy surfaces in vitro. We hypothesized that LPS would inhibit bone cell attachment either through loss of cellular attachment sites or alteration of cellular function. Three experimental approaches were used. First, alloy surfaces were exposed to LPS (100 microgram/mL) before the cells were allowed to attach. In the second approach, the cells were exposed to the LPS before they were allowed to attach. Last, the cells were allowed to attach before exposure to LPS. Cellular attachment to implant materials was measured by using a histochemical stain (MTT). The results indicated that LPS presence did not significantly (P > .05) alter osteoblast attachment to titanium or titanium alloy surfaces whether the exposure occurred before or after cellular adherence. It was concluded that LPS did not directly effect the attachment of the MC3T3-E1 osteoblasts to these implant surfaces in vitro. Further research is needed to define the clinical liabilities of LPS during implant placement and maintenance.

UR - http://www.scopus.com/inward/record.url?scp=0035553567&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035553567&partnerID=8YFLogxK

U2 - 10.1563/1548-1336(2001)027<0174:EOLCOT>2.3.CO;2

DO - 10.1563/1548-1336(2001)027<0174:EOLCOT>2.3.CO;2

M3 - Article

C2 - 12500875

AN - SCOPUS:0035553567

VL - 27

SP - 174

EP - 179

JO - Journal of Oral Implantology

JF - Journal of Oral Implantology

SN - 0160-6972

IS - 4

ER -