Effect of Matrix Metalloproteinase-inhibiting Solutions and Aging Methods on Dentin Bond Strength

Letícia C C Costa Perote, Maria B eatriz Beber Kamozaki, Natália C. Gutierrez, Franklin Chi Meng Tay, Cesar R. Pucci

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

PURPOSE: This study examined the effects of matrix metalloproteinase-inhibiting solutions and aging methods on the bond strength between resin composite and human dentin.

MATERIALS AND METHODS: Crown segments of 105 human non-carious molars were bonded using simulated pulpal pressure at 20 cm water pressure. The teeth were randomly split into 5 groups according to the solution applied: CG (control, no solution), CHX (0.2% chlorhexidine), EPE (10% ethanolic propolis extract), APE (aqueous propolis extract), and E (70% ethanol). Each solution was left on the acid-etched dentin for 1 min. Adper Single Bond 2 and resin composite (Filtek Z350 XT) were applied to all specimens. The 5 groups were subdivided according to the aging method: SI (sectioned immediately); S (storage in artificial saliva for 6 months); and T (thermomechanical aging with 240,000 mechanical cycles and 1000 thermal cycles). Specimens were sectioned into sticks and subjected to microtensile testing. Bond strength data were analyzed by two-factor ANOVA followed by a post-hoc Tukey's test (α=0.05).

RESULTS: For the factor "solution", there was no significant difference among the groups (p=0.32). For the factor "aging method", significant differences were found (p

CONCLUSIONS: The use of matrix metalloproteinase-inhibiting solutions on dentin as an adjunct to the application of an etch-and-rinse adhesive does not prevent the loss of bond strength after aging. Nevertheless, these solutions have no adverse effect on adhesion to tooth structure.

Original languageEnglish (US)
Pages (from-to)347-352
Number of pages6
JournalThe journal of adhesive dentistry
Volume17
Issue number4
DOIs
StatePublished - Aug 1 2015

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Dentin
Matrix Metalloproteinases
Propolis
Composite Resins
Tooth
Artificial Saliva
Pressure
Chlorhexidine
Crowns
Adhesives
Analysis of Variance
Ethanol
Hot Temperature
Acids
Water

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Effect of Matrix Metalloproteinase-inhibiting Solutions and Aging Methods on Dentin Bond Strength. / Perote, Letícia C C Costa; Kamozaki, Maria B eatriz Beber; Gutierrez, Natália C.; Tay, Franklin Chi Meng; Pucci, Cesar R.

In: The journal of adhesive dentistry, Vol. 17, No. 4, 01.08.2015, p. 347-352.

Research output: Contribution to journalArticle

Perote, Letícia C C Costa ; Kamozaki, Maria B eatriz Beber ; Gutierrez, Natália C. ; Tay, Franklin Chi Meng ; Pucci, Cesar R. / Effect of Matrix Metalloproteinase-inhibiting Solutions and Aging Methods on Dentin Bond Strength. In: The journal of adhesive dentistry. 2015 ; Vol. 17, No. 4. pp. 347-352.
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