Effect of mercury(II) on Nrf2, thioredoxin reductase-1 and thioredoxin-1 in human monocytes

John C. Wataha, Jill B. Lewis, Veronica V. McCloud, Melissa Shaw, Yo Omata, Petra E. Lockwood, Regina L W Messer, Jason M. Hansen

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Objectives: Human blood levels of mercury are commonly 10 nM, but may transiently reach 50-75 nM after dental amalgam placement or removal. Controversy persists about the use of mercury because the effects of these 'trace' levels of mercury are not clear. Concentrations of mercury ≥5000 nM unequivocally alter redox balance in blood cells including monocytes. In the current study, we tested a hypothesis that concentrations of mercury <100 nM altered levels and activities of key proteins that maintain monocytic redox balance. Methods: Human THP1 monocytes were exposed to 10-75 nM of Hg(II) for 6-72 h, with or without activation by lipopolysaccharide (LPS). The redox management proteins Nrf2 and thioredoxin-1 (Trx1) were separated by electrophoresis, then quantified by immunoblotting. The activity of the seleno-enzyme thioredoxin reductase (TrxR1), important in maintaining Trx1 redox balance, was measured by cell-free and cell-dependent assays. Results: Concentrations of Hg(II) between 10-75 nM increased Nrf2 levels (3.5-4.5 fold) and decreased Trx1 levels (2-3 fold), but these changes persisted <24 h. Hg(II) potently inhibited (at concentrations of 5-50 nM) TrxR1 activity in both cell-free and intracellular assays. Furthermore, Hg(II) transiently amplified LPS-induced Nrf2 levels by 2-3 fold and limited LPS-induced decreases in Trx1. All effects of Hg(II) were mitigated by pre-adding N-acetyl-cysteine (NAC) or sodium selenide (Na2SeO3), supplements of cellular thiols and selenols, respectively. Significance: Our results suggest that nanomolar concentrations of Hg(II) transiently alter cellular redox balance in monocytes that trigger changes in Nrf2 and Trx1 levels. These changes indicate that monocytes have a capacity to adapt to trace concentrations of Hg(II) that are introduced into the bloodstream after dental amalgam procedures or fish consumption. The ability of monocytes to adapt suggests that low levels of mercury exposure from dental amalgam may not overtly compromise monocyte function.

Original languageEnglish (US)
Pages (from-to)765-772
Number of pages8
JournalDental Materials
Volume24
Issue number6
DOIs
StatePublished - Jun 1 2008

Fingerprint

Thioredoxin Reductase 1
Thioredoxins
Mercury
Dental amalgams
Monocytes
Oxidation-Reduction
Dental Amalgam
Lipopolysaccharides
Assays
Blood
Acetylcysteine
Proteins
Mercury (metal)
Thioredoxin-Disulfide Reductase
Electrophoresis
Fish
Immunoblotting
Sulfhydryl Compounds
Enzymes
Chemical activation

Keywords

  • Biocompatibility
  • Cytotoxicity
  • Dental amalgam
  • Hg(II)
  • Mercury
  • Nrf2
  • Redox balance
  • Thioredoxin reductase-1
  • Thioredoxin-1
  • Trx1
  • TrxR1

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Wataha, J. C., Lewis, J. B., McCloud, V. V., Shaw, M., Omata, Y., Lockwood, P. E., ... Hansen, J. M. (2008). Effect of mercury(II) on Nrf2, thioredoxin reductase-1 and thioredoxin-1 in human monocytes. Dental Materials, 24(6), 765-772. https://doi.org/10.1016/j.dental.2007.09.002

Effect of mercury(II) on Nrf2, thioredoxin reductase-1 and thioredoxin-1 in human monocytes. / Wataha, John C.; Lewis, Jill B.; McCloud, Veronica V.; Shaw, Melissa; Omata, Yo; Lockwood, Petra E.; Messer, Regina L W; Hansen, Jason M.

In: Dental Materials, Vol. 24, No. 6, 01.06.2008, p. 765-772.

Research output: Contribution to journalArticle

Wataha, JC, Lewis, JB, McCloud, VV, Shaw, M, Omata, Y, Lockwood, PE, Messer, RLW & Hansen, JM 2008, 'Effect of mercury(II) on Nrf2, thioredoxin reductase-1 and thioredoxin-1 in human monocytes', Dental Materials, vol. 24, no. 6, pp. 765-772. https://doi.org/10.1016/j.dental.2007.09.002
Wataha, John C. ; Lewis, Jill B. ; McCloud, Veronica V. ; Shaw, Melissa ; Omata, Yo ; Lockwood, Petra E. ; Messer, Regina L W ; Hansen, Jason M. / Effect of mercury(II) on Nrf2, thioredoxin reductase-1 and thioredoxin-1 in human monocytes. In: Dental Materials. 2008 ; Vol. 24, No. 6. pp. 765-772.
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AU - Shaw, Melissa

AU - Omata, Yo

AU - Lockwood, Petra E.

AU - Messer, Regina L W

AU - Hansen, Jason M.

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N2 - Objectives: Human blood levels of mercury are commonly 10 nM, but may transiently reach 50-75 nM after dental amalgam placement or removal. Controversy persists about the use of mercury because the effects of these 'trace' levels of mercury are not clear. Concentrations of mercury ≥5000 nM unequivocally alter redox balance in blood cells including monocytes. In the current study, we tested a hypothesis that concentrations of mercury <100 nM altered levels and activities of key proteins that maintain monocytic redox balance. Methods: Human THP1 monocytes were exposed to 10-75 nM of Hg(II) for 6-72 h, with or without activation by lipopolysaccharide (LPS). The redox management proteins Nrf2 and thioredoxin-1 (Trx1) were separated by electrophoresis, then quantified by immunoblotting. The activity of the seleno-enzyme thioredoxin reductase (TrxR1), important in maintaining Trx1 redox balance, was measured by cell-free and cell-dependent assays. Results: Concentrations of Hg(II) between 10-75 nM increased Nrf2 levels (3.5-4.5 fold) and decreased Trx1 levels (2-3 fold), but these changes persisted <24 h. Hg(II) potently inhibited (at concentrations of 5-50 nM) TrxR1 activity in both cell-free and intracellular assays. Furthermore, Hg(II) transiently amplified LPS-induced Nrf2 levels by 2-3 fold and limited LPS-induced decreases in Trx1. All effects of Hg(II) were mitigated by pre-adding N-acetyl-cysteine (NAC) or sodium selenide (Na2SeO3), supplements of cellular thiols and selenols, respectively. Significance: Our results suggest that nanomolar concentrations of Hg(II) transiently alter cellular redox balance in monocytes that trigger changes in Nrf2 and Trx1 levels. These changes indicate that monocytes have a capacity to adapt to trace concentrations of Hg(II) that are introduced into the bloodstream after dental amalgam procedures or fish consumption. The ability of monocytes to adapt suggests that low levels of mercury exposure from dental amalgam may not overtly compromise monocyte function.

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KW - Thioredoxin-1

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KW - TrxR1

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