Effect of moderate caloric restriction and/or weight cycling on mammary tumor incidence and latency in MMTV-Neu female mice

Kristin A. Pape-Ansorge, Joseph P. Grande, Trace A. Christensen, Nita Jane Maihle, Margot P. Cleary

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Recently, we reported that intermittent caloric restriction-refeeding reduces mammary tumor (MT) incidence and extends latency in murine mammary tumor virus (MMTV)-transforming growth factor (TGF)-α mice to a greater extent than does chronic caloric restriction. Here, this same weight-cycling protocol was applied to MMTV-Neu female mice, which develop MTs at a much younger age than do TGF-α mice. This study consisted of three experimental groups: mice fed an AIN-93M diet ad libitum, mice intermittently fed an AIN-93 modified diet (2-fold increase in protein, fat, vitamins, and minerals) at 50% of the amount fed to the ad libitum-fed mice for 3-wk intervals and then fed an AIN-93M diet ad libitum for 3-wk intervals, and mice chronically, restricted, pair fed to the intermittently restricted mice by feeding 2:1 mixtures of AIN-93M-AIN-93 modified diets for each 6-wk feeding interval. Mice were euthanized when MTs reached a length of 20 mm or at 80 wk of age. Cumulative caloric intake was 10% lower (not significant) for intermittently restricted mice and 16% lower (P < 0.05) for chronically restricted mice than for ad libitum-fed mice. Final body weights were significantly different as follows: ad libitum-fed > intermittently restricted > chronically restricted. Fat pad weights were greater in ad libitum-fed than in intermittently restricted and chronically restricted mice. MT incidence of ad libitum-fed mice was 37% compared with 22% for intermittently restricted mice and 33% for chronically restricted mice (not significant). There were no differences in MT weight or number among the groups. These results indicate that intermittent caloric restriction-refeeding provides a moderate protective effect, whereas chronic caloric restriction provides no significant protection against MT development in transgenic Neu mice.

Original languageEnglish (US)
Pages (from-to)161-168
Number of pages8
JournalNutrition and Cancer
Volume44
Issue number2
StatePublished - Dec 1 2002
Externally publishedYes

Fingerprint

Gammaretrovirus
Caloric Restriction
Breast Neoplasms
Weights and Measures
Incidence
Diet
Transforming Growth Factors

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Oncology
  • Nutrition and Dietetics
  • Cancer Research

Cite this

Pape-Ansorge, K. A., Grande, J. P., Christensen, T. A., Maihle, N. J., & Cleary, M. P. (2002). Effect of moderate caloric restriction and/or weight cycling on mammary tumor incidence and latency in MMTV-Neu female mice. Nutrition and Cancer, 44(2), 161-168.

Effect of moderate caloric restriction and/or weight cycling on mammary tumor incidence and latency in MMTV-Neu female mice. / Pape-Ansorge, Kristin A.; Grande, Joseph P.; Christensen, Trace A.; Maihle, Nita Jane; Cleary, Margot P.

In: Nutrition and Cancer, Vol. 44, No. 2, 01.12.2002, p. 161-168.

Research output: Contribution to journalArticle

Pape-Ansorge, KA, Grande, JP, Christensen, TA, Maihle, NJ & Cleary, MP 2002, 'Effect of moderate caloric restriction and/or weight cycling on mammary tumor incidence and latency in MMTV-Neu female mice', Nutrition and Cancer, vol. 44, no. 2, pp. 161-168.
Pape-Ansorge, Kristin A. ; Grande, Joseph P. ; Christensen, Trace A. ; Maihle, Nita Jane ; Cleary, Margot P. / Effect of moderate caloric restriction and/or weight cycling on mammary tumor incidence and latency in MMTV-Neu female mice. In: Nutrition and Cancer. 2002 ; Vol. 44, No. 2. pp. 161-168.
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