Effect of nanolayering of calcium salts of phosphoric acid ester monomers on the durability of resin-dentin bonds

Fucong Tian, Xiao Yan Wang, Qi Huang, Li Na Niu, Jan Karen Mitchell, Zheng Yi Zhang, Chandrani Prananik, Lu Zhang, Ji Hua Chen, Lorenzo Breshi, David Henry Pashley, Franklin Chi Meng Tay

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

To investigate the contribution of nanolayering on resin-dentin bond durability, two phosphoric acid ester resin monomers, 10-methacryloyloxy-decyl-dihydrogen-phosphate (10-MDP) or its analog, methacryloyloxy-penta-propyleneglycol-dihydrogen-phosphate (MDA), were examined for their affinity for mineralized dentin powder in a column chromatography setup. Hydroxyapatite (HA) powder was dispersed in experimental primers consisting of 10-MDP or MDA solvated in ethanol/water and examined with FTIR, 31P MAS-NMR and XPS. Light-curable 10-MDP or MDA primers were used for bonding to dentin, and examined after 24 h or one-year of water-aging by TEM for evidence of nanolayering, and for microtensile bond strength evaluation. Primer-bonded dentin was examined by thin-film XRD to identify short-range order peaks characteristic of nanolayering of resin monomer-Ca salts. Although 10-MDP had better affinity for mineralized dentin than MDA, both monomers completely eluted from the mineralized dentin powder column using ethanol-water as mobile phase, indicating that the adsorption processes were reversible. This finding was supported by chemoanalytic data. XRD of 10-MDP-bonded dentin showed three diffraction peaks hat were absent from MDA-bonded dentin. Nanolayering was identified by TEM in 10-MDP-bonded dentin, but not in MDA-bonded dentin. Significant drop in bond strength (in MPa) was observed for both groups after one-year of water-aging compared with 24-h: 10-MDP group from 48.3 ± 6.3 to 37.4 ± 4.6; MDA group from 50.7 ± 5.0 to 35.7 ± 3.8 (P < 0.05), with no significant difference between the two groups at the same time-point. Because both functional monomer-primed, resin-bonded dentin exhibited similar bond strength decline after water-aging, presence of nanolayering is unlikely to contribute to the overall resin-dentin bond durability. Statement of Significance The durability of resin-dentin bonds in 10-MDP containing self-etching adhesives has been anecdotally attributed to the presence of nanolayering of 10-MDP-calcium salts in the resin-dentin interface. Results of the present work indicate that such a claim cannot be justified. Complete elution of the phosphoric acid ester monomer from mineralized dentin powder in the column chromatography experiments using ethanol-water mobile phase to simulate the solvent mixture employed in most 10-MDP-containing dentin adhesives further challenges the previously proposed adhesion-decalcification concept that utilizes chemical bonding of phosphoric acid ester monomers to apatite as a bonding mechanism in 10-MDP containing dentin adhesives.

Original languageEnglish (US)
Pages (from-to)190-200
Number of pages11
JournalActa biomaterialia
Volume38
DOIs
StatePublished - Jul 1 2016

Fingerprint

Organophosphates
Phosphoric acid
Dentin
Calcium
Esters
Phosphates
Durability
Resins
Salts
Monomers
Powders
Water
Adhesives
Column chromatography
Ethanol
Aging of materials
Transmission electron microscopy
Apatites
Chromatography
Apatite

Keywords

  • 10-MDP
  • Analog
  • Bond durability
  • Nanolayering
  • Organic-inorganic hybrid calcium salt
  • Phosphoric acid ester resin monomer

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

Cite this

Effect of nanolayering of calcium salts of phosphoric acid ester monomers on the durability of resin-dentin bonds. / Tian, Fucong; Wang, Xiao Yan; Huang, Qi; Niu, Li Na; Mitchell, Jan Karen; Zhang, Zheng Yi; Prananik, Chandrani; Zhang, Lu; Chen, Ji Hua; Breshi, Lorenzo; Pashley, David Henry; Tay, Franklin Chi Meng.

In: Acta biomaterialia, Vol. 38, 01.07.2016, p. 190-200.

Research output: Contribution to journalArticle

Tian, Fucong ; Wang, Xiao Yan ; Huang, Qi ; Niu, Li Na ; Mitchell, Jan Karen ; Zhang, Zheng Yi ; Prananik, Chandrani ; Zhang, Lu ; Chen, Ji Hua ; Breshi, Lorenzo ; Pashley, David Henry ; Tay, Franklin Chi Meng. / Effect of nanolayering of calcium salts of phosphoric acid ester monomers on the durability of resin-dentin bonds. In: Acta biomaterialia. 2016 ; Vol. 38. pp. 190-200.
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T1 - Effect of nanolayering of calcium salts of phosphoric acid ester monomers on the durability of resin-dentin bonds

AU - Tian, Fucong

AU - Wang, Xiao Yan

AU - Huang, Qi

AU - Niu, Li Na

AU - Mitchell, Jan Karen

AU - Zhang, Zheng Yi

AU - Prananik, Chandrani

AU - Zhang, Lu

AU - Chen, Ji Hua

AU - Breshi, Lorenzo

AU - Pashley, David Henry

AU - Tay, Franklin Chi Meng

PY - 2016/7/1

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N2 - To investigate the contribution of nanolayering on resin-dentin bond durability, two phosphoric acid ester resin monomers, 10-methacryloyloxy-decyl-dihydrogen-phosphate (10-MDP) or its analog, methacryloyloxy-penta-propyleneglycol-dihydrogen-phosphate (MDA), were examined for their affinity for mineralized dentin powder in a column chromatography setup. Hydroxyapatite (HA) powder was dispersed in experimental primers consisting of 10-MDP or MDA solvated in ethanol/water and examined with FTIR, 31P MAS-NMR and XPS. Light-curable 10-MDP or MDA primers were used for bonding to dentin, and examined after 24 h or one-year of water-aging by TEM for evidence of nanolayering, and for microtensile bond strength evaluation. Primer-bonded dentin was examined by thin-film XRD to identify short-range order peaks characteristic of nanolayering of resin monomer-Ca salts. Although 10-MDP had better affinity for mineralized dentin than MDA, both monomers completely eluted from the mineralized dentin powder column using ethanol-water as mobile phase, indicating that the adsorption processes were reversible. This finding was supported by chemoanalytic data. XRD of 10-MDP-bonded dentin showed three diffraction peaks hat were absent from MDA-bonded dentin. Nanolayering was identified by TEM in 10-MDP-bonded dentin, but not in MDA-bonded dentin. Significant drop in bond strength (in MPa) was observed for both groups after one-year of water-aging compared with 24-h: 10-MDP group from 48.3 ± 6.3 to 37.4 ± 4.6; MDA group from 50.7 ± 5.0 to 35.7 ± 3.8 (P < 0.05), with no significant difference between the two groups at the same time-point. Because both functional monomer-primed, resin-bonded dentin exhibited similar bond strength decline after water-aging, presence of nanolayering is unlikely to contribute to the overall resin-dentin bond durability. Statement of Significance The durability of resin-dentin bonds in 10-MDP containing self-etching adhesives has been anecdotally attributed to the presence of nanolayering of 10-MDP-calcium salts in the resin-dentin interface. Results of the present work indicate that such a claim cannot be justified. Complete elution of the phosphoric acid ester monomer from mineralized dentin powder in the column chromatography experiments using ethanol-water mobile phase to simulate the solvent mixture employed in most 10-MDP-containing dentin adhesives further challenges the previously proposed adhesion-decalcification concept that utilizes chemical bonding of phosphoric acid ester monomers to apatite as a bonding mechanism in 10-MDP containing dentin adhesives.

AB - To investigate the contribution of nanolayering on resin-dentin bond durability, two phosphoric acid ester resin monomers, 10-methacryloyloxy-decyl-dihydrogen-phosphate (10-MDP) or its analog, methacryloyloxy-penta-propyleneglycol-dihydrogen-phosphate (MDA), were examined for their affinity for mineralized dentin powder in a column chromatography setup. Hydroxyapatite (HA) powder was dispersed in experimental primers consisting of 10-MDP or MDA solvated in ethanol/water and examined with FTIR, 31P MAS-NMR and XPS. Light-curable 10-MDP or MDA primers were used for bonding to dentin, and examined after 24 h or one-year of water-aging by TEM for evidence of nanolayering, and for microtensile bond strength evaluation. Primer-bonded dentin was examined by thin-film XRD to identify short-range order peaks characteristic of nanolayering of resin monomer-Ca salts. Although 10-MDP had better affinity for mineralized dentin than MDA, both monomers completely eluted from the mineralized dentin powder column using ethanol-water as mobile phase, indicating that the adsorption processes were reversible. This finding was supported by chemoanalytic data. XRD of 10-MDP-bonded dentin showed three diffraction peaks hat were absent from MDA-bonded dentin. Nanolayering was identified by TEM in 10-MDP-bonded dentin, but not in MDA-bonded dentin. Significant drop in bond strength (in MPa) was observed for both groups after one-year of water-aging compared with 24-h: 10-MDP group from 48.3 ± 6.3 to 37.4 ± 4.6; MDA group from 50.7 ± 5.0 to 35.7 ± 3.8 (P < 0.05), with no significant difference between the two groups at the same time-point. Because both functional monomer-primed, resin-bonded dentin exhibited similar bond strength decline after water-aging, presence of nanolayering is unlikely to contribute to the overall resin-dentin bond durability. Statement of Significance The durability of resin-dentin bonds in 10-MDP containing self-etching adhesives has been anecdotally attributed to the presence of nanolayering of 10-MDP-calcium salts in the resin-dentin interface. Results of the present work indicate that such a claim cannot be justified. Complete elution of the phosphoric acid ester monomer from mineralized dentin powder in the column chromatography experiments using ethanol-water mobile phase to simulate the solvent mixture employed in most 10-MDP-containing dentin adhesives further challenges the previously proposed adhesion-decalcification concept that utilizes chemical bonding of phosphoric acid ester monomers to apatite as a bonding mechanism in 10-MDP containing dentin adhesives.

KW - 10-MDP

KW - Analog

KW - Bond durability

KW - Nanolayering

KW - Organic-inorganic hybrid calcium salt

KW - Phosphoric acid ester resin monomer

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