Effect of nifedipine on gingival enlargement and periodontal breakdown in ligature-induced periodontitis in rats

Marilene Issa Fernandes, Eduardo José Gaio, Cristiano Susin, Cassiano Kuchenbecker Rösing, Rui Vicente Oppermann, Pantelis Varvaki Rados

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objective: A recent consensus report could not find specific reports of the effect of nifedipine on the destruction of the periodontal tissues. The aim of the present study was to evaluate the effect of nifedipine on gingival enlargement and periodontal breakdown using a ligature-induced periodontitis in rats. Materials and methods: Fifty, male, 60 days old, Wistar rats, were divided into six groups. Cotton sutures were placed around the upper second molars. Two groups of 10 rats each did not receive ligatures and were treated daily with either saline solution or nifedipine 50 mg/kg/day. Two groups of 10 rats received ligatures and were also treated daily with saline solution or nifedipine 50 mg/kg/day. Two additional groups (nifedipine 10 and 100 mg/kg/day) were included to explore a possible dose-response relationship. Animals were euthanatised at 30 days. Internal and oral epithelium, total and inflamed connective tissue, gingival thickness and height, and bone loss were assessed histologically. Results: Nifedipine alone was not sufficient to promote gingival enlargement or periodontal destruction in the absence of the ligature. Compared to animals with ligatures only, the group that received ligatures and nifedipine 50 mg/kg/day showed significant higher estimates for total and inflamed connective tissue, gingival thickness and height. No significant differences were observed for bone loss between these experimental groups. The other dosages of nifedipine did not provide additional information. Conclusion: Nifedipine itself did not lead to gingival enlargement in rats. In the presence of biofilm accumulation, nifedipine yielded greater gingival enlargement and periodontal inflammation, but it did not increase periodontal destruction.

Original languageEnglish (US)
Pages (from-to)523-529
Number of pages7
JournalArchives of Oral Biology
Volume55
Issue number7
DOIs
StatePublished - Jul 1 2010

Fingerprint

Periodontitis
Nifedipine
Ligation
Sodium Chloride
Connective Tissue
Bone and Bones
Biofilms
Sutures
Wistar Rats
Epithelium
Inflammation

Keywords

  • Alveolar bone loss
  • Gingival overgrowth
  • Nifedipine
  • Periodontitis
  • Rats

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

Cite this

Effect of nifedipine on gingival enlargement and periodontal breakdown in ligature-induced periodontitis in rats. / Fernandes, Marilene Issa; Gaio, Eduardo José; Susin, Cristiano; Rösing, Cassiano Kuchenbecker; Oppermann, Rui Vicente; Rados, Pantelis Varvaki.

In: Archives of Oral Biology, Vol. 55, No. 7, 01.07.2010, p. 523-529.

Research output: Contribution to journalArticle

Fernandes, Marilene Issa ; Gaio, Eduardo José ; Susin, Cristiano ; Rösing, Cassiano Kuchenbecker ; Oppermann, Rui Vicente ; Rados, Pantelis Varvaki. / Effect of nifedipine on gingival enlargement and periodontal breakdown in ligature-induced periodontitis in rats. In: Archives of Oral Biology. 2010 ; Vol. 55, No. 7. pp. 523-529.
@article{a70ee1b5b21342a8b6295dfc67f264b3,
title = "Effect of nifedipine on gingival enlargement and periodontal breakdown in ligature-induced periodontitis in rats",
abstract = "Objective: A recent consensus report could not find specific reports of the effect of nifedipine on the destruction of the periodontal tissues. The aim of the present study was to evaluate the effect of nifedipine on gingival enlargement and periodontal breakdown using a ligature-induced periodontitis in rats. Materials and methods: Fifty, male, 60 days old, Wistar rats, were divided into six groups. Cotton sutures were placed around the upper second molars. Two groups of 10 rats each did not receive ligatures and were treated daily with either saline solution or nifedipine 50 mg/kg/day. Two groups of 10 rats received ligatures and were also treated daily with saline solution or nifedipine 50 mg/kg/day. Two additional groups (nifedipine 10 and 100 mg/kg/day) were included to explore a possible dose-response relationship. Animals were euthanatised at 30 days. Internal and oral epithelium, total and inflamed connective tissue, gingival thickness and height, and bone loss were assessed histologically. Results: Nifedipine alone was not sufficient to promote gingival enlargement or periodontal destruction in the absence of the ligature. Compared to animals with ligatures only, the group that received ligatures and nifedipine 50 mg/kg/day showed significant higher estimates for total and inflamed connective tissue, gingival thickness and height. No significant differences were observed for bone loss between these experimental groups. The other dosages of nifedipine did not provide additional information. Conclusion: Nifedipine itself did not lead to gingival enlargement in rats. In the presence of biofilm accumulation, nifedipine yielded greater gingival enlargement and periodontal inflammation, but it did not increase periodontal destruction.",
keywords = "Alveolar bone loss, Gingival overgrowth, Nifedipine, Periodontitis, Rats",
author = "Fernandes, {Marilene Issa} and Gaio, {Eduardo Jos{\'e}} and Cristiano Susin and R{\"o}sing, {Cassiano Kuchenbecker} and Oppermann, {Rui Vicente} and Rados, {Pantelis Varvaki}",
year = "2010",
month = "7",
day = "1",
doi = "10.1016/j.archoralbio.2010.05.003",
language = "English (US)",
volume = "55",
pages = "523--529",
journal = "Archives of Oral Biology",
issn = "0003-9969",
publisher = "Elsevier Limited",
number = "7",

}

TY - JOUR

T1 - Effect of nifedipine on gingival enlargement and periodontal breakdown in ligature-induced periodontitis in rats

AU - Fernandes, Marilene Issa

AU - Gaio, Eduardo José

AU - Susin, Cristiano

AU - Rösing, Cassiano Kuchenbecker

AU - Oppermann, Rui Vicente

AU - Rados, Pantelis Varvaki

PY - 2010/7/1

Y1 - 2010/7/1

N2 - Objective: A recent consensus report could not find specific reports of the effect of nifedipine on the destruction of the periodontal tissues. The aim of the present study was to evaluate the effect of nifedipine on gingival enlargement and periodontal breakdown using a ligature-induced periodontitis in rats. Materials and methods: Fifty, male, 60 days old, Wistar rats, were divided into six groups. Cotton sutures were placed around the upper second molars. Two groups of 10 rats each did not receive ligatures and were treated daily with either saline solution or nifedipine 50 mg/kg/day. Two groups of 10 rats received ligatures and were also treated daily with saline solution or nifedipine 50 mg/kg/day. Two additional groups (nifedipine 10 and 100 mg/kg/day) were included to explore a possible dose-response relationship. Animals were euthanatised at 30 days. Internal and oral epithelium, total and inflamed connective tissue, gingival thickness and height, and bone loss were assessed histologically. Results: Nifedipine alone was not sufficient to promote gingival enlargement or periodontal destruction in the absence of the ligature. Compared to animals with ligatures only, the group that received ligatures and nifedipine 50 mg/kg/day showed significant higher estimates for total and inflamed connective tissue, gingival thickness and height. No significant differences were observed for bone loss between these experimental groups. The other dosages of nifedipine did not provide additional information. Conclusion: Nifedipine itself did not lead to gingival enlargement in rats. In the presence of biofilm accumulation, nifedipine yielded greater gingival enlargement and periodontal inflammation, but it did not increase periodontal destruction.

AB - Objective: A recent consensus report could not find specific reports of the effect of nifedipine on the destruction of the periodontal tissues. The aim of the present study was to evaluate the effect of nifedipine on gingival enlargement and periodontal breakdown using a ligature-induced periodontitis in rats. Materials and methods: Fifty, male, 60 days old, Wistar rats, were divided into six groups. Cotton sutures were placed around the upper second molars. Two groups of 10 rats each did not receive ligatures and were treated daily with either saline solution or nifedipine 50 mg/kg/day. Two groups of 10 rats received ligatures and were also treated daily with saline solution or nifedipine 50 mg/kg/day. Two additional groups (nifedipine 10 and 100 mg/kg/day) were included to explore a possible dose-response relationship. Animals were euthanatised at 30 days. Internal and oral epithelium, total and inflamed connective tissue, gingival thickness and height, and bone loss were assessed histologically. Results: Nifedipine alone was not sufficient to promote gingival enlargement or periodontal destruction in the absence of the ligature. Compared to animals with ligatures only, the group that received ligatures and nifedipine 50 mg/kg/day showed significant higher estimates for total and inflamed connective tissue, gingival thickness and height. No significant differences were observed for bone loss between these experimental groups. The other dosages of nifedipine did not provide additional information. Conclusion: Nifedipine itself did not lead to gingival enlargement in rats. In the presence of biofilm accumulation, nifedipine yielded greater gingival enlargement and periodontal inflammation, but it did not increase periodontal destruction.

KW - Alveolar bone loss

KW - Gingival overgrowth

KW - Nifedipine

KW - Periodontitis

KW - Rats

UR - http://www.scopus.com/inward/record.url?scp=77955427392&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955427392&partnerID=8YFLogxK

U2 - 10.1016/j.archoralbio.2010.05.003

DO - 10.1016/j.archoralbio.2010.05.003

M3 - Article

C2 - 20538258

AN - SCOPUS:77955427392

VL - 55

SP - 523

EP - 529

JO - Archives of Oral Biology

JF - Archives of Oral Biology

SN - 0003-9969

IS - 7

ER -