Effect of nitric oxide on mitogen-activated protein kinases in neonatal pulmonary vascular smooth muscle

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Mitogen-activated protein kinases (MAPKs) belong to the group of serine/threonine kinases that are rapidly activated in response to growth factor stimulation. In adult mammalian cells, the MAPK family includes extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2 or p44mapk and p42mapk), which translocate to the nucleus and integrate signals from second messengers leading to cellular proliferation or differentiation. However, the specific role of MAPKs in neonatal pulmonary vascular smooth muscle is not well understood. Expression of p44mapk and p42mapk in primary cultured pulmonary vascular smooth muscle cells from neonatal (1-2 day old) rats was identified by Western immunoblot analysis. Treatment with 10 nM endothelin-1 (ET-1), a potent vasoconstrictor with vascular mitogenic properties, induced phosphorylation of both p44mapk and p42 mapk, but treatment with the exogenous nitric oxide (NO) donor sodium nitroprusside inhibited both p44mapk and p42mapk phosphorylation by ET-1. The specific cGMP-dependent protein kinase (PKG) inhibitor KT5823, the nonspecific nitric oxide synthase (NOS) inhibitor L-NAME, and the specific NOS 1 blocker NPLA all significantly enhanced both P44 mapk and p42mapk phosphorylation by ET-1. Collectively, these data demonstrate the expression and phosphorylation of specific MAPKs in rat neonatal pulmonary vascular smooth muscle and suggests that the NO signaling pathway modulates MAPK activation by ET-1.

Original languageEnglish (US)
Pages (from-to)325-335
Number of pages11
JournalLung
Volume183
Issue number5
DOIs
StatePublished - Oct 1 2005

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Mitogen-Activated Protein Kinases
Vascular Smooth Muscle
Endothelin-1
Nitric Oxide
Lung
Phosphorylation
Mitogen-Activated Protein Kinase 1
Nitric Oxide Synthase
Cyclic GMP-Dependent Protein Kinases
Mitogen-Activated Protein Kinase 3
Nitric Oxide Donors
Protein-Serine-Threonine Kinases
NG-Nitroarginine Methyl Ester
Nitroprusside
Second Messenger Systems
Vasoconstrictor Agents
Protein Kinase Inhibitors
Smooth Muscle Myocytes
Blood Vessels
Intercellular Signaling Peptides and Proteins

Keywords

  • Endothelin-1
  • Mitogen-activated protein kinase
  • Neonatal
  • Nos isozymes
  • Pulmonary vascular smooth muscle

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Physiology

Cite this

Effect of nitric oxide on mitogen-activated protein kinases in neonatal pulmonary vascular smooth muscle. / Barman, Scott A.

In: Lung, Vol. 183, No. 5, 01.10.2005, p. 325-335.

Research output: Contribution to journalArticle

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