Effect of nonsurgical periodontal therapy on serum and gingival crevicular fluid cytokine levels during pregnancy and postpartum

T. Fiorini, C. Susin, J. M. da Rocha, P. Weidlich, P. Vianna, C. H.C. Moreira, J. A. Bogo Chies, C. K. Rösing, R. V. Oppermann

Research output: Contribution to journalArticle

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Abstract

Background and Objective: A low-grade systemic inflammatory status originating from periodontal infection has been proposed to explain the association between periodontal disease and systemic conditions, including adverse obstetric outcomes. The aim of this study was to evaluate the effect of periodontal therapy during pregnancy on the gingival crevicular fluid and serum levels of six cytokines associated with periodontal disease and preterm birth. Material and Methods: A subsample of 60 women (18-35years of age) up to 20 gestational weeks, previously enrolled in a larger randomized clinical trial, was recruited for the present study. Participants were randomly allocated to receive either comprehensive nonsurgical periodontal therapy before 24 gestational weeks (n=30, test group) or only one appointment for supragingival calculus removal (n=30, control group). Clinical data, and samples of blood and gingival crevicular fluid, were collected at baseline, at 26-28 gestational weeks and 30d after delivery. The levels of interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12p70 and tumor necrosis factor-α were analyzed by flow cytometry. Results: After treatment, a major reduction in periodontal inflammation was observed in the test group, with bleeding on probing decreasing from 49.62% of sites to 11.66% of sites (p<0.001). Periodontal therapy significantly reduced the levels of IL-1β and IL-8 in gingival crevicular fluid (p<0.001). However, no significant effect of therapy was observed on serum cytokine levels. After delivery, the levels of IL-1β in the gingival crevicular fluid of the test group were significantly lower than were those in the control group (p<0.001), but there were no significant differences between test and control groups regarding serum cytokine levels. Conclusion: Although periodontal therapy during pregnancy successfully reduced periodontal inflammation and gingival crevicular fluid cytokine levels, it did not have a significant impact on serum biomarkers.

Original languageEnglish (US)
Pages (from-to)126-133
Number of pages8
JournalJournal of Periodontal Research
Volume48
Issue number1
DOIs
StatePublished - Feb 1 2013

Fingerprint

Gingival Crevicular Fluid
Postpartum Period
Cytokines
Pregnancy
Serum
Interleukin-1
Periodontal Diseases
Interleukin-8
Control Groups
Therapeutics
Inflammation
Interleukins
Premature Birth
Calculi
Interleukin-10
Obstetrics
Interleukin-6
Appointments and Schedules
Flow Cytometry
Randomized Controlled Trials

Keywords

  • Cytokines
  • Inflammation
  • Inflammatory mediator
  • Nonsurgical periodontal therapy
  • Periodontal medicine
  • Systemic host effect

ASJC Scopus subject areas

  • Periodontics

Cite this

Effect of nonsurgical periodontal therapy on serum and gingival crevicular fluid cytokine levels during pregnancy and postpartum. / Fiorini, T.; Susin, C.; da Rocha, J. M.; Weidlich, P.; Vianna, P.; Moreira, C. H.C.; Bogo Chies, J. A.; Rösing, C. K.; Oppermann, R. V.

In: Journal of Periodontal Research, Vol. 48, No. 1, 01.02.2013, p. 126-133.

Research output: Contribution to journalArticle

Fiorini, T, Susin, C, da Rocha, JM, Weidlich, P, Vianna, P, Moreira, CHC, Bogo Chies, JA, Rösing, CK & Oppermann, RV 2013, 'Effect of nonsurgical periodontal therapy on serum and gingival crevicular fluid cytokine levels during pregnancy and postpartum', Journal of Periodontal Research, vol. 48, no. 1, pp. 126-133. https://doi.org/10.1111/j.1600-0765.2012.01513.x
Fiorini, T. ; Susin, C. ; da Rocha, J. M. ; Weidlich, P. ; Vianna, P. ; Moreira, C. H.C. ; Bogo Chies, J. A. ; Rösing, C. K. ; Oppermann, R. V. / Effect of nonsurgical periodontal therapy on serum and gingival crevicular fluid cytokine levels during pregnancy and postpartum. In: Journal of Periodontal Research. 2013 ; Vol. 48, No. 1. pp. 126-133.
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abstract = "Background and Objective: A low-grade systemic inflammatory status originating from periodontal infection has been proposed to explain the association between periodontal disease and systemic conditions, including adverse obstetric outcomes. The aim of this study was to evaluate the effect of periodontal therapy during pregnancy on the gingival crevicular fluid and serum levels of six cytokines associated with periodontal disease and preterm birth. Material and Methods: A subsample of 60 women (18-35years of age) up to 20 gestational weeks, previously enrolled in a larger randomized clinical trial, was recruited for the present study. Participants were randomly allocated to receive either comprehensive nonsurgical periodontal therapy before 24 gestational weeks (n=30, test group) or only one appointment for supragingival calculus removal (n=30, control group). Clinical data, and samples of blood and gingival crevicular fluid, were collected at baseline, at 26-28 gestational weeks and 30d after delivery. The levels of interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12p70 and tumor necrosis factor-α were analyzed by flow cytometry. Results: After treatment, a major reduction in periodontal inflammation was observed in the test group, with bleeding on probing decreasing from 49.62{\%} of sites to 11.66{\%} of sites (p<0.001). Periodontal therapy significantly reduced the levels of IL-1β and IL-8 in gingival crevicular fluid (p<0.001). However, no significant effect of therapy was observed on serum cytokine levels. After delivery, the levels of IL-1β in the gingival crevicular fluid of the test group were significantly lower than were those in the control group (p<0.001), but there were no significant differences between test and control groups regarding serum cytokine levels. Conclusion: Although periodontal therapy during pregnancy successfully reduced periodontal inflammation and gingival crevicular fluid cytokine levels, it did not have a significant impact on serum biomarkers.",
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AU - Susin, C.

AU - da Rocha, J. M.

AU - Weidlich, P.

AU - Vianna, P.

AU - Moreira, C. H.C.

AU - Bogo Chies, J. A.

AU - Rösing, C. K.

AU - Oppermann, R. V.

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AB - Background and Objective: A low-grade systemic inflammatory status originating from periodontal infection has been proposed to explain the association between periodontal disease and systemic conditions, including adverse obstetric outcomes. The aim of this study was to evaluate the effect of periodontal therapy during pregnancy on the gingival crevicular fluid and serum levels of six cytokines associated with periodontal disease and preterm birth. Material and Methods: A subsample of 60 women (18-35years of age) up to 20 gestational weeks, previously enrolled in a larger randomized clinical trial, was recruited for the present study. Participants were randomly allocated to receive either comprehensive nonsurgical periodontal therapy before 24 gestational weeks (n=30, test group) or only one appointment for supragingival calculus removal (n=30, control group). Clinical data, and samples of blood and gingival crevicular fluid, were collected at baseline, at 26-28 gestational weeks and 30d after delivery. The levels of interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12p70 and tumor necrosis factor-α were analyzed by flow cytometry. Results: After treatment, a major reduction in periodontal inflammation was observed in the test group, with bleeding on probing decreasing from 49.62% of sites to 11.66% of sites (p<0.001). Periodontal therapy significantly reduced the levels of IL-1β and IL-8 in gingival crevicular fluid (p<0.001). However, no significant effect of therapy was observed on serum cytokine levels. After delivery, the levels of IL-1β in the gingival crevicular fluid of the test group were significantly lower than were those in the control group (p<0.001), but there were no significant differences between test and control groups regarding serum cytokine levels. Conclusion: Although periodontal therapy during pregnancy successfully reduced periodontal inflammation and gingival crevicular fluid cytokine levels, it did not have a significant impact on serum biomarkers.

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