It was found that the difference in the partial pressure of oxygen (pO2) resulting from the distinct altitude over the sea level, between Baltimore, Md., USA and Mexico City, about 2,240 meters, is sufficient to cause variance in conjugation frequency in two haploid strains of Kluyveromyces lactis. Further studies confirm that sexual conjugation in K. lactis is highly sensitive to changes in the pO2. Ergosterol which depends on O2 for its biosynthesis, stimulates the mating process, as does the ergosterol precursor, squalene. Ergosterol and O2 effects were found to be additive, suggesting that O2 may influence conjugation in more than one way. Culture, storage and transfer frequency modify both, mating efficiency and the response to O2 and lipid supplement.
|Original language||English (US)|
|Number of pages||8|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Nov 27 1974|
ASJC Scopus subject areas
- Molecular Biology