TY - JOUR
T1 - Effect of O2, ergosterol and sterol precursors on the mating ability of Kluyveromyces lactis
AU - Mas, Jaime
AU - Celis, Esteban
AU - Piña, Enrique
AU - Brunner, Aurora
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1974/11/27
Y1 - 1974/11/27
N2 - It was found that the difference in the partial pressure of oxygen (pO2) resulting from the distinct altitude over the sea level, between Baltimore, Md., USA and Mexico City, about 2,240 meters, is sufficient to cause variance in conjugation frequency in two haploid strains of Kluyveromyces lactis. Further studies confirm that sexual conjugation in K. lactis is highly sensitive to changes in the pO2. Ergosterol which depends on O2 for its biosynthesis, stimulates the mating process, as does the ergosterol precursor, squalene. Ergosterol and O2 effects were found to be additive, suggesting that O2 may influence conjugation in more than one way. Culture, storage and transfer frequency modify both, mating efficiency and the response to O2 and lipid supplement.
AB - It was found that the difference in the partial pressure of oxygen (pO2) resulting from the distinct altitude over the sea level, between Baltimore, Md., USA and Mexico City, about 2,240 meters, is sufficient to cause variance in conjugation frequency in two haploid strains of Kluyveromyces lactis. Further studies confirm that sexual conjugation in K. lactis is highly sensitive to changes in the pO2. Ergosterol which depends on O2 for its biosynthesis, stimulates the mating process, as does the ergosterol precursor, squalene. Ergosterol and O2 effects were found to be additive, suggesting that O2 may influence conjugation in more than one way. Culture, storage and transfer frequency modify both, mating efficiency and the response to O2 and lipid supplement.
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U2 - 10.1016/0006-291X(74)91001-8
DO - 10.1016/0006-291X(74)91001-8
M3 - Article
C2 - 4455239
AN - SCOPUS:0016135833
SN - 0006-291X
VL - 61
SP - 613
EP - 620
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -