Effect of oxidized regenerated cellulose (Interceed®) on the expression of tissue plasminogen activator and plasminogen activator inhibitor-1 in human peritoneal fibroblasts and mesothelial cells

L. April Gago, Ghassan Saed, Eslam Elhammady, Michael Peter Diamond

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Objective: To characterize the molecular changes that occur in normal fibroblasts, adhesion fibroblasts, and mesothelial cells as a result of exposure to oxidized regenerated cellulose (Interceed®; Johnson & Johnson Medical, Inc., New Brunswick, NJ). Design: Control and Interceed®-treated normal peritoneal fibroblasts, adhesion fibroblasts, and mesothelial cells in culture were assessed for messenger RNA levels of molecules known to be associated with adhesion development, using multiplex reverse transcriptase polymerase chain reaction (n = 4). Setting: University research laboratory. Patient(s): Normal and adhesion fibroblasts and mesothelial cells. Intervention(s): Exposure of cells, normal fibroblasts, adhesion fibroblasts, and mesothelial cells to oxidized regenerated cellulose. Main Outcome Measure(s): Real-time reverse transcriptase polymerase chain reaction expression of messenger RNA tissue plasminogen activator (tPA), plasminogen activator inhibitor-1 (PAI-1), and tPA-PAI-1 ratio, an indicator of overall fibrinolytic activity. Result(s): Interceed® treatment of normal peritoneal fibroblasts, adhesion fibroblasts, and mesothelial cells results in an increased expression of tPA in mesothelial cells and an increase in the tPA-PAI-1 ratio, signifying an overall increase in fibrinolytic activity. Conclusion(s): Interceed®, which has been shown in multiple human in vivo studies to decrease postoperative adhesion development, increases the expression of tPA and the tPA-PAI-1 ratio (an indicator of overall fibrinolytic activity), thereby promoting dissolution of fibrin and healing without adhesion development. Thus, the ability of Interceed® to reduce postoperative adhesion development may be derived from both a barrier and biologic effect.

Original languageEnglish (US)
Pages (from-to)1223-1227
Number of pages5
JournalFertility and Sterility
Volume86
Issue number4 SUPPL.
DOIs
StatePublished - Oct 1 2006
Externally publishedYes

Fingerprint

oxidized cellulose
Plasminogen Activator Inhibitor 1
Tissue Plasminogen Activator
Fibroblasts
Reverse Transcriptase Polymerase Chain Reaction
New Brunswick
Messenger RNA
Multiplex Polymerase Chain Reaction
Fibrin
Real-Time Polymerase Chain Reaction
Cell Culture Techniques
Outcome Assessment (Health Care)

Keywords

  • Adhesions
  • Interceed®
  • PAI-1
  • adhesion barriers
  • adhesion fibroblasts
  • fibrinolysis
  • mesothelial cells
  • tPA

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

@article{c6ad403c784b4a90901bed4b565a46cc,
title = "Effect of oxidized regenerated cellulose (Interceed{\circledR}) on the expression of tissue plasminogen activator and plasminogen activator inhibitor-1 in human peritoneal fibroblasts and mesothelial cells",
abstract = "Objective: To characterize the molecular changes that occur in normal fibroblasts, adhesion fibroblasts, and mesothelial cells as a result of exposure to oxidized regenerated cellulose (Interceed{\circledR}; Johnson & Johnson Medical, Inc., New Brunswick, NJ). Design: Control and Interceed{\circledR}-treated normal peritoneal fibroblasts, adhesion fibroblasts, and mesothelial cells in culture were assessed for messenger RNA levels of molecules known to be associated with adhesion development, using multiplex reverse transcriptase polymerase chain reaction (n = 4). Setting: University research laboratory. Patient(s): Normal and adhesion fibroblasts and mesothelial cells. Intervention(s): Exposure of cells, normal fibroblasts, adhesion fibroblasts, and mesothelial cells to oxidized regenerated cellulose. Main Outcome Measure(s): Real-time reverse transcriptase polymerase chain reaction expression of messenger RNA tissue plasminogen activator (tPA), plasminogen activator inhibitor-1 (PAI-1), and tPA-PAI-1 ratio, an indicator of overall fibrinolytic activity. Result(s): Interceed{\circledR} treatment of normal peritoneal fibroblasts, adhesion fibroblasts, and mesothelial cells results in an increased expression of tPA in mesothelial cells and an increase in the tPA-PAI-1 ratio, signifying an overall increase in fibrinolytic activity. Conclusion(s): Interceed{\circledR}, which has been shown in multiple human in vivo studies to decrease postoperative adhesion development, increases the expression of tPA and the tPA-PAI-1 ratio (an indicator of overall fibrinolytic activity), thereby promoting dissolution of fibrin and healing without adhesion development. Thus, the ability of Interceed{\circledR} to reduce postoperative adhesion development may be derived from both a barrier and biologic effect.",
keywords = "Adhesions, Interceed{\circledR}, PAI-1, adhesion barriers, adhesion fibroblasts, fibrinolysis, mesothelial cells, tPA",
author = "Gago, {L. April} and Ghassan Saed and Eslam Elhammady and Diamond, {Michael Peter}",
year = "2006",
month = "10",
day = "1",
doi = "10.1016/j.fertnstert.2006.04.021",
language = "English (US)",
volume = "86",
pages = "1223--1227",
journal = "Fertility and Sterility",
issn = "0015-0282",
publisher = "Elsevier Inc.",
number = "4 SUPPL.",

}

TY - JOUR

T1 - Effect of oxidized regenerated cellulose (Interceed®) on the expression of tissue plasminogen activator and plasminogen activator inhibitor-1 in human peritoneal fibroblasts and mesothelial cells

AU - Gago, L. April

AU - Saed, Ghassan

AU - Elhammady, Eslam

AU - Diamond, Michael Peter

PY - 2006/10/1

Y1 - 2006/10/1

N2 - Objective: To characterize the molecular changes that occur in normal fibroblasts, adhesion fibroblasts, and mesothelial cells as a result of exposure to oxidized regenerated cellulose (Interceed®; Johnson & Johnson Medical, Inc., New Brunswick, NJ). Design: Control and Interceed®-treated normal peritoneal fibroblasts, adhesion fibroblasts, and mesothelial cells in culture were assessed for messenger RNA levels of molecules known to be associated with adhesion development, using multiplex reverse transcriptase polymerase chain reaction (n = 4). Setting: University research laboratory. Patient(s): Normal and adhesion fibroblasts and mesothelial cells. Intervention(s): Exposure of cells, normal fibroblasts, adhesion fibroblasts, and mesothelial cells to oxidized regenerated cellulose. Main Outcome Measure(s): Real-time reverse transcriptase polymerase chain reaction expression of messenger RNA tissue plasminogen activator (tPA), plasminogen activator inhibitor-1 (PAI-1), and tPA-PAI-1 ratio, an indicator of overall fibrinolytic activity. Result(s): Interceed® treatment of normal peritoneal fibroblasts, adhesion fibroblasts, and mesothelial cells results in an increased expression of tPA in mesothelial cells and an increase in the tPA-PAI-1 ratio, signifying an overall increase in fibrinolytic activity. Conclusion(s): Interceed®, which has been shown in multiple human in vivo studies to decrease postoperative adhesion development, increases the expression of tPA and the tPA-PAI-1 ratio (an indicator of overall fibrinolytic activity), thereby promoting dissolution of fibrin and healing without adhesion development. Thus, the ability of Interceed® to reduce postoperative adhesion development may be derived from both a barrier and biologic effect.

AB - Objective: To characterize the molecular changes that occur in normal fibroblasts, adhesion fibroblasts, and mesothelial cells as a result of exposure to oxidized regenerated cellulose (Interceed®; Johnson & Johnson Medical, Inc., New Brunswick, NJ). Design: Control and Interceed®-treated normal peritoneal fibroblasts, adhesion fibroblasts, and mesothelial cells in culture were assessed for messenger RNA levels of molecules known to be associated with adhesion development, using multiplex reverse transcriptase polymerase chain reaction (n = 4). Setting: University research laboratory. Patient(s): Normal and adhesion fibroblasts and mesothelial cells. Intervention(s): Exposure of cells, normal fibroblasts, adhesion fibroblasts, and mesothelial cells to oxidized regenerated cellulose. Main Outcome Measure(s): Real-time reverse transcriptase polymerase chain reaction expression of messenger RNA tissue plasminogen activator (tPA), plasminogen activator inhibitor-1 (PAI-1), and tPA-PAI-1 ratio, an indicator of overall fibrinolytic activity. Result(s): Interceed® treatment of normal peritoneal fibroblasts, adhesion fibroblasts, and mesothelial cells results in an increased expression of tPA in mesothelial cells and an increase in the tPA-PAI-1 ratio, signifying an overall increase in fibrinolytic activity. Conclusion(s): Interceed®, which has been shown in multiple human in vivo studies to decrease postoperative adhesion development, increases the expression of tPA and the tPA-PAI-1 ratio (an indicator of overall fibrinolytic activity), thereby promoting dissolution of fibrin and healing without adhesion development. Thus, the ability of Interceed® to reduce postoperative adhesion development may be derived from both a barrier and biologic effect.

KW - Adhesions

KW - Interceed®

KW - PAI-1

KW - adhesion barriers

KW - adhesion fibroblasts

KW - fibrinolysis

KW - mesothelial cells

KW - tPA

UR - http://www.scopus.com/inward/record.url?scp=33748883853&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33748883853&partnerID=8YFLogxK

U2 - 10.1016/j.fertnstert.2006.04.021

DO - 10.1016/j.fertnstert.2006.04.021

M3 - Article

VL - 86

SP - 1223

EP - 1227

JO - Fertility and Sterility

JF - Fertility and Sterility

SN - 0015-0282

IS - 4 SUPPL.

ER -