Effect of Parathyroid Hormone and Calcitonin on Cholinergic Markers in Rat Parathyroid Gland

Javier E. Stern, Daniel P. Cardinali

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

The effect of parathyroid hormone (PTH) and calcitonin on 3H‐choline uptake and on 3H‐acetylcholine synthesis by rat parathyroid glands were examined. Incubation of tissue for 120 min with PTH or calcitonin resulted in an inverted bell‐shaped, dose‐dependent inhibition of 3H‐choline uptake, with a maximal effect at 10−9 M Concentration. The effect of PTH on parathyroid choline uptake was blunted by preincubation with the PTH antagonist NLe8–18 ‐PTH (3–34) amide. PTH brought about a dose‐dependent inhibition of 3H‐choline conversion to 3H‐acetylcholine, with significant effects at 10−8 M concentration or higher. This inhibitory effect of PTH on 3H‐acetylcholine synthesis was blocked by co‐incubation with the PTH antagonist NLe8–18‐PTH (3–34) amide. Only at a 10−7 M concentration calcitonin was effective to impair the in vitro conversion of radioactive choline into 3H‐acetylcholine by parathyroid fragments. The results indicate that, in vitro, PTH, and to a less extent, calcitonin, inhibit cholinergic activity in rat parathyroid glands.

Original languageEnglish (US)
Pages (from-to)689-693
Number of pages5
JournalJournal of Neuroendocrinology
Volume7
Issue number9
DOIs
StatePublished - Sep 1995

Keywords

  • acetylcholine
  • calcitonin
  • parasympathetic nervous system
  • parathyroid glands
  • parathyroid hormone

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Endocrine and Autonomic Systems
  • Cellular and Molecular Neuroscience

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