Effect of pH on dentin protease inactivation by carbodiimide

Roda Seseogullari-Dirihan, Mustafa M. Mutluay, Leo Tjäderhane, Lorenzo Breschi, David Henry Pashley, Arzu Tezvergil-Mutluay

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A water-soluble crosslinking agent, 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC), has been used as a pretreatment of acid-etched dentin to inactivate matrix-bound endogenous dentin proteases. The aim of this study was to evaluate the effect of pH on the inactivation capacity of EDC. Demineralized dentin beams (1 × 2 × 6 mm) were divided into six groups (n = 8 per group). Then, EDC (0.3 M) was solubilized in distilled water with pH of 2, 4, 6, 7, 9, or 11. Control EDC was solubilized in 0.1 M 2-(N-morpholino) ethanesulfonic acid (MES) buffer and its pH was adjusted to 6. The dentin beams were pretreated for 1 min with EDC at each pH or with EDC in MES buffer at pH 6.0 and then incubated in 1 ml of simulated body fluid (pH 7.2) for 1, 3, 7, or 14 d. Untreated beams served as controls. At each study time-point, the dry mass of dentin beams was assessed and the incubation media were analyzed for carboxyterminal telopeptide of type-I collagen (ICTP) and C-terminal telopeptide of type I collagen (CTX) using specific ELISAs. Data were subjected to repeat-measures anova. The results of the study indicated that specimens pretreated with EDC in MES buffer showed the lowest collagen degradation in terms of mass loss and release of telopeptides, while specimens pretreated in alkaline media showed the highest collagen degradation. This study indicates that the pH of the EDC solution plays an important role in the stability of dentin protease inactivation.

Original languageEnglish (US)
Pages (from-to)288-293
Number of pages6
JournalEuropean Journal of Oral Sciences
Volume125
Issue number4
DOIs
StatePublished - Aug 1 2017

Fingerprint

Carbodiimides
Dentin
Peptide Hydrolases
Buffers
Collagen
Ethyldimethylaminopropyl Carbodiimide
Water
Body Fluids
Enzyme-Linked Immunosorbent Assay
Acids
2-(N-morpholino)ethanesulfonic acid

Keywords

  • CTX
  • ICTP
  • carbodiimide
  • collagen degradation
  • proteases inhibition

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Seseogullari-Dirihan, R., Mutluay, M. M., Tjäderhane, L., Breschi, L., Pashley, D. H., & Tezvergil-Mutluay, A. (2017). Effect of pH on dentin protease inactivation by carbodiimide. European Journal of Oral Sciences, 125(4), 288-293. https://doi.org/10.1111/eos.12354

Effect of pH on dentin protease inactivation by carbodiimide. / Seseogullari-Dirihan, Roda; Mutluay, Mustafa M.; Tjäderhane, Leo; Breschi, Lorenzo; Pashley, David Henry; Tezvergil-Mutluay, Arzu.

In: European Journal of Oral Sciences, Vol. 125, No. 4, 01.08.2017, p. 288-293.

Research output: Contribution to journalArticle

Seseogullari-Dirihan, R, Mutluay, MM, Tjäderhane, L, Breschi, L, Pashley, DH & Tezvergil-Mutluay, A 2017, 'Effect of pH on dentin protease inactivation by carbodiimide', European Journal of Oral Sciences, vol. 125, no. 4, pp. 288-293. https://doi.org/10.1111/eos.12354
Seseogullari-Dirihan R, Mutluay MM, Tjäderhane L, Breschi L, Pashley DH, Tezvergil-Mutluay A. Effect of pH on dentin protease inactivation by carbodiimide. European Journal of Oral Sciences. 2017 Aug 1;125(4):288-293. https://doi.org/10.1111/eos.12354
Seseogullari-Dirihan, Roda ; Mutluay, Mustafa M. ; Tjäderhane, Leo ; Breschi, Lorenzo ; Pashley, David Henry ; Tezvergil-Mutluay, Arzu. / Effect of pH on dentin protease inactivation by carbodiimide. In: European Journal of Oral Sciences. 2017 ; Vol. 125, No. 4. pp. 288-293.
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