Abstract
It is becoming increasingly difficult to obtain sufficient freshly extracted human teeth to utilize in in vitro studies, particularly for bonding investigations. The purpose of this study was to investigate the effect of different storage conditions for human extracted teeth prior to their use in microtensile bond strength (μTBS) testing. Thirty incisors were divided into groups according to their storage time and condition. The first group consisted of freshly extracted teeth. Teeth of the second group were stored in chloramine-T at 4°C for 2 years ±1 month. The last group was stored dry for up to 2 years ±2 months, after which teeth were rehydrated in distilled water for 2 weeks before use. All teeth were disinfected using 0.5 chloramine-T. Dentin surfaces were exposed and then bonded using Scotchbond Multi-Purpose or Clearfil SE Bond to composite resin foundations (Esthet-X). Following 24-hour storage, bonded teeth (n=30) were sectioned into beams with a cross-section of 0.9 ±0.1 mm 2 and subjected to μTBS testing using a microtensile testing machine (Micro Tensile Tester). Data were calculated and statistically analyzed using Kruskal-Wallis and Mann-Whitney tests (α=.05). Failure modes were determined using a stereomicroscope at ×40 magnification. Statistical analysis revealed no significant difference between the different storage conditions nor between the 2 adhesive systems for each storage condition (P>.05). Modes of failure for all tested groups were predominantly adhesive followed by mixed. Within the limitations of this study, it was concluded that aging of teeth and storage conditions had no significant effect on dentin microtensile bond strength to composite resin.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 92-97 |
| Number of pages | 6 |
| Journal | Journal of Prosthetic Dentistry |
| Volume | 104 |
| Issue number | 2 |
| DOIs | |
| State | Published - Aug 20 2010 |
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ASJC Scopus subject areas
- Oral Surgery
Cite this
Effect of pretest storage conditions of extracted teeth on their dentin bond strengths. / Mobarak, Enas H.; El-Badrawy, Wafa; Pashley, David Henry; Jamjoom, Hana.
In: Journal of Prosthetic Dentistry, Vol. 104, No. 2, 20.08.2010, p. 92-97.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of pretest storage conditions of extracted teeth on their dentin bond strengths
AU - Mobarak, Enas H.
AU - El-Badrawy, Wafa
AU - Pashley, David Henry
AU - Jamjoom, Hana
PY - 2010/8/20
Y1 - 2010/8/20
N2 - It is becoming increasingly difficult to obtain sufficient freshly extracted human teeth to utilize in in vitro studies, particularly for bonding investigations. The purpose of this study was to investigate the effect of different storage conditions for human extracted teeth prior to their use in microtensile bond strength (μTBS) testing. Thirty incisors were divided into groups according to their storage time and condition. The first group consisted of freshly extracted teeth. Teeth of the second group were stored in chloramine-T at 4°C for 2 years ±1 month. The last group was stored dry for up to 2 years ±2 months, after which teeth were rehydrated in distilled water for 2 weeks before use. All teeth were disinfected using 0.5 chloramine-T. Dentin surfaces were exposed and then bonded using Scotchbond Multi-Purpose or Clearfil SE Bond to composite resin foundations (Esthet-X). Following 24-hour storage, bonded teeth (n=30) were sectioned into beams with a cross-section of 0.9 ±0.1 mm 2 and subjected to μTBS testing using a microtensile testing machine (Micro Tensile Tester). Data were calculated and statistically analyzed using Kruskal-Wallis and Mann-Whitney tests (α=.05). Failure modes were determined using a stereomicroscope at ×40 magnification. Statistical analysis revealed no significant difference between the different storage conditions nor between the 2 adhesive systems for each storage condition (P>.05). Modes of failure for all tested groups were predominantly adhesive followed by mixed. Within the limitations of this study, it was concluded that aging of teeth and storage conditions had no significant effect on dentin microtensile bond strength to composite resin.
AB - It is becoming increasingly difficult to obtain sufficient freshly extracted human teeth to utilize in in vitro studies, particularly for bonding investigations. The purpose of this study was to investigate the effect of different storage conditions for human extracted teeth prior to their use in microtensile bond strength (μTBS) testing. Thirty incisors were divided into groups according to their storage time and condition. The first group consisted of freshly extracted teeth. Teeth of the second group were stored in chloramine-T at 4°C for 2 years ±1 month. The last group was stored dry for up to 2 years ±2 months, after which teeth were rehydrated in distilled water for 2 weeks before use. All teeth were disinfected using 0.5 chloramine-T. Dentin surfaces were exposed and then bonded using Scotchbond Multi-Purpose or Clearfil SE Bond to composite resin foundations (Esthet-X). Following 24-hour storage, bonded teeth (n=30) were sectioned into beams with a cross-section of 0.9 ±0.1 mm 2 and subjected to μTBS testing using a microtensile testing machine (Micro Tensile Tester). Data were calculated and statistically analyzed using Kruskal-Wallis and Mann-Whitney tests (α=.05). Failure modes were determined using a stereomicroscope at ×40 magnification. Statistical analysis revealed no significant difference between the different storage conditions nor between the 2 adhesive systems for each storage condition (P>.05). Modes of failure for all tested groups were predominantly adhesive followed by mixed. Within the limitations of this study, it was concluded that aging of teeth and storage conditions had no significant effect on dentin microtensile bond strength to composite resin.
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U2 - 10.1016/S0022-3913(10)60098-4
DO - 10.1016/S0022-3913(10)60098-4
M3 - Article
VL - 104
SP - 92
EP - 97
JO - Journal of Prosthetic Dentistry
JF - Journal of Prosthetic Dentistry
SN - 0022-3913
IS - 2
ER -